数字农科院2.0

Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)

文献类型： 外文期刊

作者： Pang, C. Y.;Deng, T. X.;Yang, C. Y.;Yang, B. Z.;Liang, X. W.;Tang, D. S.;Jiang, H.

作者机构：

关键词： Molecular cloning;GC-rich;rDNA;rRNA;Bubalus bubalis

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2012 年 11 卷 3 期

页码：

收录情况： SCI

摘要： The rDNA genes coding for ribosomal RNA in animals are complicated repeat sequences with high GC content. We amplified water buffalo rDNA gene sequences with the long and accurate (LA) PCR method, using LA Taq DNA polymerase and GC buffer, based on bioinformatic analysis of related organisms. The rDNA genes were found to consist of 9016 nucleotides, including three rRNA genes and two internal transcribed spacers (ITS), which we named 18S rRNA, ITS1, 5.8S rRNA, ITS2 and 28S rRNA. We tested and optimized conditions for cloning these complicated rDNA sequences, including specific rules of primer design, improvements in the reaction system, and selection of the DNA polymerase.

分类号：

  • 相关文献

[1]细菌转录调控蛋白CarD的研究进展. 吴港,王金辉,张维,林敏,陈明,张云华. 2014

[2]牛病毒性腹泻病毒、宋内氏痢疾杆菌混合感染致仔鹿腹泻的研究 被引量:1. 乔连江,张萍,程悦宁,刘泽余,张海威,周玉成,周曼丽,徐莉,侯庆丰,杨艳玲. 2019

[3]Characterization Of Circular Rnas In C.hinese Buffalo (Bubalus Bubalis) A dipose Tissue: A Focus On Circular Rnas Involved In Fat Deposition. Wei, Xuefeng,Huang, Jieping,Li, Fen,Ma, Yun,Zheng, Qiuzhi,Huang, Jieping,Lei, Chuzhao,Wang, Shuzhe,Ma, Yun,Shang, Jianghua,Zhao, Jinhui. 2019

[4]Strong and stable geographic differentiation of swamp buffalo maternal and paternal lineages indicates domestication in the China/Indochina border region. Zhang, Yi,Lu, Yongfang,Li, Qiang,Wang, Yachun,Zhang, Yuan,Yindee, Marnoch,Li, Kuan-Yi,Ju, Yu-Ten,Kuo, Hsiao-Yun,Ye, Shaohui,Faruque, Md Omar,Vu Chi Cuong,Lan Doan Pham,Bouahom, Bounthong,Yang, Bingzhuang,Liang, Xianwei,Cai, Zhihua,Vankan, Dianne,Manatchaiworakul, Wallaya,Kowlim, Nonglid,Wajjwalku, Worawidh,Duangchantrasiri, Somphot,Colenbrander, Ben,Lenstra, Johannes A.,Beerli, Peter,Barker, J. Stuart F..

[5]Prevalence Of Bovine Tuberculosis In B.uffaloes In Hyderabad And T ando Allahyar Districts Of Sindh Province, Pakistan. Malhi, KK, Kamboh, AA, Dewani, P, Kumar, C, Abro, SH, Leghari, A, Shoaib, M. 2018

[6]Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size. Liang Jing,Li Na,Zhang Long-chao,Wang Li-gang,Liu Xin,Zhao Ka-bin,Yan Hua,Pu Lei,Zhang Yue-bo,Shi Hui-bi,Wang Li-xian,Li Na,Zhang Qin. 2016

[7]Molecular cloning, characterization and mRNA expression analysis of a novel selenoprotein: avian selenoprotein W from chicken. Li, Jin-Long,Ruan, Hong-Feng,Li, Hui-Xin,Xu, Shi-Wen,Tang, Zhao-Xin,Li, Jin-Long,Tang, Zhao-Xin,Li, Hui-Xin. 2011

[8]Cytogenetic relationships among Citrullus species in comparison with some genera of the tribe Benincaseae (Cucurbitaceae) as inferred from rDNA distribution patterns. Li, Kun-Peng,Wu, Yun-Xiang,Wang, Yan,Lu, Xing-Ming,Li, Zong-Yun,Han, Yong-Hua,Zhao, Hong,Xu, Yong,Wang, Ji-Ming. 2016

[9]Genetic polymorphism of ferula mushroom growing on Ferula sinkiangensis. Zhang, JX,Huang, CY,Ng, TB,Wang, HX.

[10]Molecular cloning and characterization of a novel alpha-galactosidase gene from Penicillium sp F63 CGMCC 1669 and expression in Pichia pastoris. Mi, Shijun,Meng, Kun,Wang, Yaru,Bai, Yingguo,Yuan, Tiezheng,Luo, Huiying,Yao, Bin. 2007

[11]Molecular cloning and characterization of hatching enzyme-like gene in the silkworm, Bombyx mori. Tang, Shun-ming,Shen, Xing-jia,Zhao, Qiao-ling,Zhang, Guo-zheng,Guo, Xi-jie,Lu, Fu-hao,Wang, Na.

[12]Individual chromosome identification, chromosomal collinearity and genetic-physical integrated map in Gossypium darwinii and four D genome cotton species revealed by BAC-FISH. Yimei Gan,Fang Liu,Renhai Peng,Chunying Wang,Shaohui Li,Xiangdi Zhang,Yuhong Wang,Kunbo Wang. 2012

[13]Divergence between C-melo and African Cucumis Species Identified by Chromosome Painting and rDNA Distribution Pattern. Li, Kunpeng,Sun, Jianying,Li, Zongyun,Han, Yonghua,Wang, Huaisong,Wang, Jiming.

[14]Molecular Cloning, Expression, and Identification of Bre Genes Involved in Glycosphingolipids Synthesis in Helicoverpa armigera (Lepidoptera: Noctuidae). Xiao, Yutao,Dhiloo, Khalid Hussain,Wu, Kongming,Soberon, Mario,Bravo, Alejandra.

[15]Bipolaris eleusines, a potential mycoherbicide candidate for control of barnyardgrass ( Echinochloa crus-galli). Zhang, Jianping,Duan, Guifang,Zhou, Yongjun,Yang, Shuang,Yu, Liuqing,Peng, Gary. 2014

[16]A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR. Peng, DL,Moens, M.

[17]Molecular Cloning and Expression of a Novel Protease-resistant GH-36 alpha-Galactosidase from Rhizopus sp F78 ACCC 30795. Yanan Cao,Wang, Yaru,Luo, Huiying,Shi, Pengjun,Meng, Kun,Zhou, Zhigang,Yao, Bin,Zhang, Zhifang. 2009

[18]Expression, characterization, and antimicrobial ability of T4 lysozyme from methylotrophic yeast Hansenula polymorpha A16. Wang Nan,Li GangQiang,Sun Ning,Liu DeHu,Wang YueJu. 2011

[19]Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.). Fang, Rongjun,Hu, Dongqing,Zhang, Yinghua,Li, Long,Zhao, Weiguo,Liu, Li,Cheng, Jialin,Fang, Rongjun,Qi, Jinliang,Yang, Yonghua,Hu, Dongqing,Zhang, Yinghua,Zhao, Weiguo,Liu, Li.

[20]Accurate Chromosome Identification in the Prunus Subgenus Cerasus (Prunus pseudocerasus) and its Relatives by Oligo-FISH. Wang L.,Feng Y.,Wang Y.,Zhang J.,Chen Q.,Liu Z.,Liu C.,He W.,Wang H.,Yang S.,Zhang Y.,Luo Y.,Tang H.,Wang X.. 2022

作者其他论文 更多>>