数字农科院2.0

In vitro embryo production in buffalo (Bubalus bubalis) using sexed sperm and oocytes from ovum pick up

文献类型： 外文期刊

作者： Lu, Y. Q.;Lu, S. S.;Zhang, M.;Lu, K. H.;Liang, X. W.;Chen, M. T.;Zhang, X. F.;Pang, C. Y.;Huang, F. X.

作者机构：

关键词： buffalo;sexed sperm;in vitro embryo production;OPU;IVF

期刊名称： THERIOGENOLOGY

ISSN： 0093-691X

年卷期： 2008 年 69 卷 7 期

页码：

收录情况： SCI

摘要： The objective was to explore the use of sexed sperm and OPU-derived oocytes in an IVP system to produce sex-preselected bubaline embryos. Oocytes were recovered from 20 fertile Murrah and Nili-Ravi buffalo cows by repeated (twice weekly) ultrasound-guided transvaginal ovum pick up (OPU), or by aspiration of abbatoir-derived bubaline ovaries, and subjected to IVF, using frozen-thawed sexed or unsexed bubaline semen. On average, 4.6 oocytes were retrieved per buffalo per session (70.9% were Grades A or B). Following IVF with sexed sperm, oocytes derived from OPU had similar developmental competence as those from abattoir-derived ovaries, in terms of cleavage rate (57.6 vs. 50.4%, P = 0.357) and blastocyst development rate (16.0 vs. 23.9%, P = 0.237). Furthermore, using frozen-thawed sexed versus unsexed semen did not affect rates of cleavage (50.5 vs. 50.9%, P = 0.978) or blastocyst development (15.3 vs. 19.1 %, P = 0.291) after IVF using OPU-derived oocytes. Of the embryos produced in an OPU-IVP system, 9 of 34 sexed fresh embryos (26.5%) and 5 of 43 sexed frozen embryos (11.6%) transferred to recipients established pregnancies, whereas 7 of 26 unsexed fresh embryos (26.9%) and 6 out of 39 unsexed frozen embryos (15.4%) transferred to recipients established pregnancies. Eleven sex-preselected buffalo calves (10 females and one male) and 10 sexed buffalo calves (six females and four males) were born following embryo transfer. In the present study, OPU, sperm sexing technology, IVP, and embryo transfer, were used to produce sex-preselected buffalo calves. This study provided proof of concept for further research and wider field application of these technologies in buffalo. (c) 2008 Published by Elsevier Inc.

分类号：

  • 相关文献

[1]A Comparison of in vitro Development of Buffalo (Bubalus bubalis) Embryos Produced either by Somatic Cell Nuclear Transfer or in vitro Fertilization using Oocytes Obtained by Different Methods. Yang, Chunyan,Pang, Chunying,Zheng, Haiying,Huang, Fenxiang,Shang, Jianghua,Wang, Jian,Yang, Bingzhuang,Liang, Xianwei. 2013

[2]Optimization of cryopreservation of buffalo (Bubalus bubalis) blastocysts produced by in vitro fertilization and somatic cell nuclear transfer. Yang, C. Y.,Pang, C. Y.,Yang, B. Z.,Li, R. C.,Liang, X. W.,Lu, Y. Q..

[3]马IVF与克隆技术的研究进展. 郝海生,朱化彬. 2007

[4]OPU卵母细胞体外成熟体系的优化研究进展. 张培培,郝海生,杜卫华,朱化彬,李树静,余文莉,赵学明. 2023

[5]OVUM PICK-UP-IN VITRO EMBRYO PRODUCTION - EMBRYO TRANSFER TECHNOLOGY IN THE APPLICATION OF WATER BUFFALO PRODUCTION. Qin, Guang-Sheng,Chen, Ming-Tang,Yang, Bing-Zhuang,Huang, Jia-xiang,Pang, Chun-ying,Zhang, Xiu-fang,Liang, Xian-wei,Tan, Zheng-zhun,Li, Hui,Jiang, He-sheng. 2012

[6]Phenotypic and Genetic Analyses of In Vitro Embryo Production Traits in Chinese Holstein Cattle. Yuechuan Huang,Hailiang Zhang,Cheng Mei,Minglu Yang,Shanjiang Zhao,Huabin Zhu,Yachun Wang. 2023

[7]Trichostatin A Treatment on Two Types of Donor Cells for Somatic Cell Nuclear Transfer. Yao, Ya-Xin,Li, He-Ping,Yao, Ya-Xin,Guan, Wei-Jun,Li, Xiang-Chen,Su, Xiao-Hua,Ma, Yue-Hui. 2012

[8]Progesterone and 17 beta-estradiol, but not follicle stimulating hormone, alter the sex ratio of murine embryos fertilized in vitro. Zhang, L.,Du, W.,Lin, X.,Zhang, L.,Zhang, A.,Chen, H.. 2008

[9]Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip. Li, Jingchun,Ning, Bolin,Luo, Yinghua,Guo, Li,Wei, Guosheng,Liu, Shengjun,Zhang, Ying,Zhang, Aizhong,Wu, Rui,Li, Yanbing,Cao, Xinyan.

[10]Vaginal microbiota are associated with in vitro fertilization during female infertility. Wang, Tao,Li, Penghao,Bai, Xue,Tian, Shilin,Yang, Maosen,Leng, Dong,Kui, Hua,Zhang, Sujuan,Yan, Xiaomiao,Zheng, Qu,Luo, Pulin,He, Changming,Jia, Yan,Wu, Zhoulin,Qiu, Huimin,Li, Jing,Wan, Feng,Ali, Muhammad A.,Mao, Rurong,Liu, Yong-Xin,Li, Diyan. 2024

[11]Developmental Potential Of Buffalo Embryos Cultured In Serum Free Culture System. Shahzad, Qaisar,Liang, Xianwei,Xu, Hui-Yan,Pu, Liping,Waqas, Muhammad,Wadood, Armughan Ahmed,Xie, Long,Lu, Ke-Huan,Lu, Yangqing. 2020

[12]Developmental Competence Of Buffalo (Bubalus B.ubalis) Denuded Oocytes Cocultured W ith Cumulus Cells: Protective Role Of Cumulus Cells. Shang, Jiang-Hua,Huang, Jia-Xiang,Moussa, Mahmoud,Moussa, Mahmoud,Li, Meng-Qi,Yan, Sheng-Fei,Zheng, Hai-Ying,Yu, Nong-Qi,Yang, Chun-Yan. 2018

[13]Comparison Of Long Non-Coding Rna Expression Profiles Of Cattle And Buffalo Differing In Muscle Characteristics. Huang, Kongwei,Liu, Qingyou,Liu, Qingyou,Cui, Kuiqing,Feng, Tong,Feng, Tong,Luo, Chan,Shi, Deshun,Wang, Pengcheng,Li, Hui,Qian, Qian,Ruan, Jue,Shafique, Laiba. 2020

[14]Nanos2 Is A Molecular Marker O.f Inchoate Buffalo Spermatogonia. Luo, Ao-Lin,Lu, Ke-Huan,Xu, Hui-Yan,Luo, Ao-Lin,Xu, Hui-Yan,Lu, Ke-Huan,Lu, Yang-Qing,Lu, Sheng-Sheng,Lu, Yang-Qing,Liang, Xing-Wei,Lu, Sheng-Sheng,Yang, Xiao-Gan,Li, Ting-Ting,Geng, Shuang-Shuang,Liang, Xing-Wei,Li, Ting-Ting,Li, Meng-Qi,Geng, Shuang-Shuang,Zhao, Peng-Wei,Zhao, Peng-Wei,Yang, Xiao-Gan,Li, Meng-Qi,Li, Meng-Qi. 2017

[15]Genome-Wide Analysis Reveals Genetic Diversity, Linkage Disequilibrium, And Selection For Milk Production Traits In Chinese Buffalo Breeds. Liang, S. S.,Li, W. Q.,Liang, X. W.,Rushdi, H. E.,Ma, X. Y.,Deng, T. X.,Duan, A. Q.,Lu, X. R.,Abdel-Shafy, H.. 2020

[16]Molecular Characterisation Of The Buffalo S.cap Gene And Its A ssociation With Milk Production Traits In Water Buffaloes. Liang, Shasha,Liang, Xianwei,Lu, Xingrong,Deng, Tingxian,Ma, Xiaoya,Pang, Chunying,Duan, Anqin. 2018

[17]Genome-Wide Snp Data Revealed The E.xtent Of Linkage Disequilibrium, P ersistence Of Phase And Effective Population Size In Purebred And Crossbred Buffalo Populations. Wang, Zhiquan,Zhang, Chunyan,Liang, Aixin,Yang, Liguo,Riaz, Hasan,Salzano, Angela,Liang, Xianwei,Ye, Tingzhu,Hua, Guohua,Deng, Tingxian,Campanile, Giuseppe,Deng, Tingxian,Gasparrini, Bianca,Plastow, Graham,Liu, Jiajia,Cassandro, Martino,Liu, Shenhe. 2019

[18]Vitrification Alters Cell Adhesion Related G.enes In Pre-Implantation Buffalo E mbryos: Protective Role Of Beta-Mercaptoethanol. Zheng, Hai-Ying,Yu, Nong-Qi,Yu, Nong-Qi,Yang, Chun-Yan,Yang, Chun-Yan,Yan, Sheng-Fei,Yan, Sheng-Fei,Shang, Jiang-Hua,Shang, Jiang-Hua,Moussa, Mahmoud,Moussa, Mahmoud,Moussa, Mahmoud,Li, Meng-Qi,Li, Meng-Qi,Huang, Jia-Xiang,Huang, Jia-Xiang,Zheng, Hai-Ying. 2019

[19]Integrative Analysis Of Transcriptome And G.was Data To Identify T he Hub Genes Associated With Milk Yield Trait In Buffalo. Hua, Guohua,Gasparrini, Bianca,Ma, Xiaoya,Liang, Aixin,Liu, Shenhe,Liang, Xianwei,Liang, Shasha,Lu, Xingrong,Neglia, Gianluca,Pang, Chunying,Salzano, Angela,Yang, Liguo,Deng, Tingxian,Campanile, Giuseppe,Duan, Anqin,Deng, Tingxian. 2019

[20]Analysis of 22 Elements in Milk, Feed, and Water of Dairy Cow, Goat, and Buffalo from Different Regions of China. Zhou, Xuewei,Qu, Xueyin,Zhao, Shengguo,Wang, Jiaqi,Li, Songli,Zheng, Nan,Zhou, Xuewei,Qu, Xueyin,Zhao, Shengguo,Wang, Jiaqi,Li, Songli,Zheng, Nan.

作者其他论文 更多>>