数字农科院2.0

A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae

文献类型： 外文期刊

作者： Jie Chen ; Yongfeng Shi ; Wenzheng Liu ; Rongyao Chai ; Yaping Fu ; Jieyun Zhuang ; Jianli Wu *

作者机构：

关键词： Oryza sativa;Magnaporthe oryzae;Blast resistance gene;NBS-LRR

期刊名称： Journal of Genetics and Genomics

ISSN： 1673-8527

年卷期： 2011 年 38 卷 5 期

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 科技核心（2020版）

摘要： Rice blast, caused by Magnaporthe oryzae, is one of the most devastating diseases. Using map-based strategy and in silico approach we isolated a new rice (Oryza sativa L.) blast resistance allele of Pid3, designated Pi25, from a stable blast resistance cultivar Gumei2. Overexpression analysis and complementation test showed that Pi25 conferred blast resistance to M. oryzae isolate js001-20. Sequence analysis showed that Pi25 was an intronless gene of 2772 nucleotides with single nucleotide substitution in comparison to Pid3 at the nucleotide position 459 and predicatively encoded a typical coiled coil-nucleotide binding site-leucine rich repeat (CC-NBS-LRR) protein of 924 amino acid residuals with 100% identity to Pid3 putative protein. The susceptible allele pi25 in Nipponbare contained a nonsense mutation at the nucleotide position 2209 resulting in a truncated protein with 736 amino acid residuals. In addition, 14 nucleotide substitutions resulting in 10 amino acid substitutions were identified between Pi25 and pi25 upstream the premature stop codon in the susceptible allele. Although the mechanism of Pi25/Pid3-mediated resistance needs to be further investigated, the isolation of the allele would facilitate the utilization of Pi25/Pid3 in rice blast resistance breeding program via transgenic approach and marker assisted selection.

分类号：

  • 相关文献

[1]Novel Insights into Rice Innate Immunity Against Bacterial and Fungal Pathogens. Wang, Guo-Liang,Liu, Jinling,Liu, Jinling,Triplett, Lindsay,Leach, Jan E.,Wang, Guo-Liang. 2014

[2]OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in Rice. Ya Chen,Zhiquan Liu,Shuai Meng,Zhenan Shen,Huanbin Shi,Jiehua Qiu,Fucheng Lin,Shu Zhang,Yanjun Kou. 2023

[3]OsEIL2 balances rice immune responses against (hemi)biotrophic and necrotrophic pathogens via the salicylic acid and jasmonic acid synergism. Zhao, Yudan,Zhu, Xiaoying,Shi, Cheng-Min,Xu, Guojuan,Zuo, Shimin,Shi, Yanlong,Cao, Wenlei,Kang, Houxiang,Liu, Wende,Wang, Ruyi,Ning, Yuese,Wang, Guo-Liang,Wang, Xuli. 2024

[4]The F-box protein OsFBX156 positively regulates rice defence against the blast fungus Magnaporthe oryzae by mediating ubiquitination-dependent degradation of OsHSP71.1. Yudan Zhao,Xionghui Zhong,Guojuan Xu,Xiaoying Zhu,Yanlong Shi,Minghao Liu,Ruyi Wang,Houxiang Kang,Xiaoman You,Yuese Ning,Guo Liang Wang,Xuli Wang. 2024

[5]Knockout of phosphatidate phosphohydrolase genes confers broad-spectrum disease resistance in plants. Gong, Qiuwen,Sha, Gan,Han, Xinyu,Guo, Zhenhua,Yang, Lei,Chen, Ting,Yang, Wei,Tan, Ronglei,Liu, Meng,Xia, Fengdie,Chen, Guang,Li, Yufei,Shen, Xin,Xie, Kabin,Cai, Guangqin,Hu, Honghong,Luo, Jie,Li, Qiang,Li, Guotian. 2024

[6]OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in Rice. Ya Chen,Zhiquan Liu,Linyin Yang,Wu Fujie,Zijian Cao,Huanbin Shi,Jiehua Qiu,Yanjun Kou. 2025

[7]麦瘟病研究进展与展望. 何心尧,郝元峰,周益林,何中虎. 2017

[8]Identification and Expression of Genes Involved in Race-specific Blast Resistance in Rice. Hu, Hai-Yan,Zheng, Kang-Le,Zhuang, Jie-Yun,Zheng, Kang-Le,Zhuang, Jie-Yun,Chai, Rong-Yao,Li, Yu-Chuan. 2010

[9]RGA法克隆NBS-LRR类抗病基因同源序列及其在葫芦科作物上应用的研究进展. 薛莹莹,孙守如,孙德玺,邓云,朱迎春,刘君璞. 2014

[10]棉花多抗品种中植棉KV-1抗病基因同源序列的克隆与分析. 王升正,齐放军,张文蔚,简桂良. 2009

[11]中国野生刺葡萄抗白腐病NBS-LRR类抗病基因同源序列的分离与鉴定. 张颖,李峰,刘崇怀,樊秀彩,孙海生,姜建福,张国海. 2013

[12]辣椒抗病基因同源序列的克隆与分析. 张丽英,陈儒钢,张俊红,欧阳波,肖景华,李汉霞,叶志彪. 2008

[13]小麦Mlo及NBS-LRR类抗病基因同源序列的分离与鉴定(英文). 李爱丽,孔秀英,周荣华,马峙英,贾继增. 2003

[14]菠菜NBS-LRR类抗病基因同源序列的克隆及分析. 折红兵,范桂彦,张合龙,王晓武,武剑,钱伟,徐兆生. 2017

[15]利用BSMV-VIGS技术快速分析小麦TNBL1基因的抗黄矮病功能. 赵继荣,黄茜,李宁,刘艳,黄占景,张增艳. 2011

[16]基于高精度转录组测序技术挖掘甘蔗抗梢腐病相关的NBS-LRR家族基因. 曾子颜,张木清,张兴坦. 2022

[17]Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat. Li, AL,Kong, XY,Zhou, RH,Ma, ZY,Jia, JZ.

[18]Isolation, characterization and phylogenetic analysis of the resistance gene analogues (RGAs) in banana (Musa spp.). Pei, Xinwu,Li, Shengjun,Jiang, Ying,Zhang, Yongqiang,Wang, Zhixing,Jia, Shirong.

[19]miR398b negatively regulates cotton immune responses to Verticillium dahliae via multiple targets. Miao, Yuhuan,Chen, Kun,Deng, Jinwu,Zhang, Lin,Wang, Weiran,Kong, Jie,Klosterman, Steven J.,Zhang, Xianlong,Aierxi, Alifu,Zhu, Longfu. 2022

[20]Fine Mapping and Characterization of an Aphid-Resistance Gene in the Soybean Landrace Fangzheng Moshidou. Yang, Jing,Liu, Guangyang,Tang, Junyong,Wang, Xiujun,Diao, Yanling,Su, Yang,Sun, Dan,Shang, Jiawei,Guo, Yong,Qiu, Li-Juan. 2022

作者其他论文 更多>>