数字农科院2.0

Molecular cloning, characterization and association analysis of the promoter region of the bovine CDK6 gene

文献类型： 外文期刊

作者： Liu, Y. F.;Zan, L. S.;Xin, Y. P.;Jiao, Y.;Cui, W. T.;Li, K.;Liu, Y. F.

作者机构：

关键词： Cattle;CDK6 gene;SNP polymorphism;Association analysis;Body measurement

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2011 年 10 卷 3 期

页码：

收录情况： SCI

摘要： Cyclin-dependent kinase 6 (CDK6) is a key element of D-type cyclin holoenzymes. It is involved in the regulation of the G1-phase of the cell cycle and is considered to be an important candidate gene for selection of body measurement traits through marker-assisted selection. We cloned the promoter sequence of this gene in bovines and found it to share high similarity with that of the human CDK6 promoter. A 2271-bp sequence upstream of the start codon in the bovine CDK6 5'-flanking sequence is rich in GC; it lacks consensus TATA or CAAT box, but it contains several MZF1 binding sites. Other potential cis-regulatory elements were found in the 5'-flanking region, including CdxA, SRY, p300, GATA-1, and deltaE. Allele frequencies were also analyzed in various cattle breeds (Qinchuan, Qinchuan improvement steers, Nanyang, Jiaxian red, Xia'nan, Luxi, Simmental and Luxi crossbred steers, and Xuelong) and association with a selected single nucleotide polymorphism (SNP) was calculated. The T-1075C SNP in the promoter was found to be significantly associated with body length and heart girth. This SNP marker was found to be significantly associated with body length and the heart girth in 737 individuals. We conclude that this SNP of the CDK6 gene has potential as a genetic marker for important body traits in bovine reproduction and breeding.

分类号：

  • 相关文献

[1]Molecular characterization, polymorphism of bovine ZBTB38 gene and association with body measurement traits in native Chinese cattle breeds. Liu, Yongfeng,Zan, Linsen,Zhao, Shuanping,Xin, Yaping,Li, Linqiang,Liu, Yongfeng,Cui, Wentao,Tang, Zhonglin,Li, Kui,Zan, Linsen.

[2]Molecular cloning, expression and characterization of bovine UQCC and its association with body measurement traits. Liu, Yongfeng,Zan, Linsen,Zhao, Shuanping,Liu, Yongfeng,Huang, Honggang,Li, Yong,Tang, Zhonglin,Yang, Shulin,Li, Kui,Zan, Linsen. 2010

[3]A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds. Liu, Yong Feng,Zan, Lin Sen,Zhao, Shuan Ping,Xin, Ya Ping,Lin, Qing,Tian, Wan Qiang,Li, Kui,Wang, Zhi Wei,Zan, Lin Sen.

[4]Molecular characterization, expression pattern, polymorphism and association analysis of bovine ADAMTSL3 gene. Liu, Yongfeng,Zan, Linsen,Zhao, Shuanping,Xin, Yaping,Jiao, Yang,Zhao, Shuanping,Li, Kui,Liu, Yongfeng.

[5]Association analysis of thyroglobulin gene variants with carcass and meat quality traits in beef cattle. Xu, Shang-Zhong,Gan, Qian-Fu,Hou, Guan-Yu,Xu, Shang-Zhong. 2008

[6]An isolation, polymorphism and trait association study of bovine GAD1 and GAL genes. Guo, H.,Li, K.,Liu, W-S.,Li, H-D.,Xu, S-Z.,Ren, H-Y.,Gao, X.. 2006

[7]Tissues expression analysis, novel SNPs of the bovine Angptl4 gene and its effects on bovine bioeconomic traits. Ma, Yun,Chen, Ningbo,Li, Rongrong,Xu, Yongjie,Li, Fen,Zheng, Xianrui,Li, Shuyuan,Zhang, Huali,Huang, Yufen,Bai, Fang,Wang, Junfang,Li, Yafang,Yang, Ting,Gao, Hanting,Wang, Xinzhuang,Li, Junya.

[8]Genetic Polymorphisms of the CACNA2D1 Gene and Their Association with Carcass and Meat Quality Traits in Cattle. Hou, Guan-Yu,Yuan, Zheng-Rong,Gao, Xue,Li, Jun-Ya,Gao, Hui-Jiang,Chen, Jin-Bao,Xu, Shang-Zhong,Hou, Guan-Yu. 2010

[9]Morphological diversities and eco-geographical structuring of Ethiopian camel (Camelus dromedarius) populations. Yosef, T.,Kefelegn, K.,Mohammed, Y. K.,Mengistu, U.,Solomon, A.,Tadelle, D.,Han, J.. 2014

[10]Analysis Of Drought Tolerance And A.ssociated Traits In Upland C otton At The Seedling Stage. Chen, Jing,Liu, Rui:Hua,Zhang, Xiao:Meng,Zhang, Si:Ping,Liu, Shao:Dong,Yang, Yong:Fei,Pang, Chao:You,Li, Yang,Ge, Chang:Wei,Li, Cun:Dong,Yang, Yong:Fei,Ju, Fei:Yan,Ma, Hui:Juan,Li, Hai:Ming,Shen, Qian,Ju, Fei:Yan,Pang, Chao:You,Li, Hai:Ming,Zhao, Xin:Hua. 2019

[11]Detection Of Favorable Alleles For Y.ield And Yield Components B y Association Mapping In Upland Cotton. Li, Baocheng,Wang, Juan,Chen, Quanjia,Yu, Yu,Dong, Chengguang,Dong, Chengguang. 2018

[12]Associations of Polymorphisms in Four Candidate Genes with Carcass and/or Meat-Quality Traits in Two Meat-Type Chicken Lines. Sun, Yanfa,Wang, Hongrong,Wen, Jie,Sun, Yanfa,Liu, Ranran,Lu, Xiqing,Hu, Yaodong,Zhao, Guiping,Zheng, Maiqing,Chen, Jilan,Wen, Jie,Liu, Ranran,Zhao, Guiping,Zheng, Maiqing,Chen, Jilan,Wen, Jie. 2013

[13]Molecular characterization and association analysis of FBXO40 with partial hematological indexes in pig. Wang, Z. W.,Li, X. Y.,Tang, Z. L.,Yang, S. L.,Ying, Z. Z.,Fu, T.,Mu, Y. L.,Ao, H.,Li, K.,Wang, Z. W.,Li, X. Y.,Ying, Z. Z.,Fu, T.,Fan, B.. 2010

[14]Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size. Liang Jing,Li Na,Zhang Long-chao,Wang Li-gang,Liu Xin,Zhao Ka-bin,Yan Hua,Pu Lei,Zhang Yue-bo,Shi Hui-bi,Wang Li-xian,Li Na,Zhang Qin. 2016

[15]LXR alpha gene expression, genetic variation and association analysis between novel SNPs and growth traits in Chinese native cattle. Ma, Yun,Chen, Ningbo,Li, Rongrong,Xu, Yongjie,Li, Fen,Li, Junya,Gao, Shuxin,Li, Xiaofeng,Shi, Kuilin. 2014

[16]Tissue expression, association analysis between three novel SNPs of the RXR alpha gene and growth traits in Chinese indigenous cattle. Ma Yun,Gao HanTing,Chen NingBo,Xu YongJie,Jiang JinHang,Li Fen,Lu FangRu,Zhao Man,Gao HanTing,Lin Feng,Jiang JinHang,Shi KuiLin,Cheng Ni,Li JunYa. 2013

[17]Identification mutation of the CACNA2D1 gene and its effect on somatic cell score in cattle. Deng, Gui-xin,Yuan, Zheng-rong,Gao, Xue,Li, Jun-ya,Chen, Jin-bao,Gao, Hui-jiang,Xu, Shang-zhong. 2011

[18]Sequence characterization, polymorphism, and chromosomal localizations of the porcine Capz genes. Yang, E.,Wang, H.,Tang, Z. L.,Yang, S. L.,Li, K.,Yang, E.,Wang, H.,Wu, X. X.. 2008

[19]Identification of promoter motifs regulating ZmeIF4E expression level involved in maize rough dwarf disease resistance in maize (Zea Mays L.). Shi, Liyu,Weng, Jianfeng,Liu, Changlin,Song, Xinyuan,Hao, Zhuanfang,Xie, Chuanxiao,Li, Mingshun,Zhang, Degui,Bai, Li,Li, Xinhai,Zhang, Shihuang,Miao, Hongqin,Pan, Guangtang. 2013

[20]Molecular characterization, expression and association analysis of the porcine CMYA4 gene with carcass traits. Xu, X. L.,Li, K.,Peng, Z. Z.,Zhao, S. H.,Yu, M.,Fan, B.,Zhu, M. J.,Liu, B.,Xu, X. L.,Li, K.,Peng, Z. Z.,Zhao, S. H.,Yu, M.,Fan, B.,Zhu, M. J.,Liu, B.,Li, K.,Xu, S. P.,Du, Y. Q.. 2008

作者其他论文 更多>>