数字农科院2.0

Cloning and Characterization of a Putative CTR1 Gene from Wheat

文献类型： 外文期刊

作者： Bi Cai-Li;Wen Xiao-Jie;Zhang Xue-Yong;Liu Xu;Bi Cai-Li

作者机构：

关键词： CTR1;RACE;stress;Triticum aestivum L.;transgenic tobacco

期刊名称： AGRICULTURAL SCIENCES IN CHINA

ISSN： 1671-2927

年卷期： 2010 年 9 卷 9 期

页码：

收录情况： SCI

摘要： CTR1 is a key negative regulator in ethylene signal transduction. A salt-induced CTR1 like gene (TaCTR1) was cloned from wheat, its expression under abiotic stresses, subcellular localization and the effect of overexpression of TaCTR1 on salt tolerance in tobacco was studied. A putative CTRI gene was cloned and characterized from wheat via rapid amplification of cDNA ends (RACE) and RT-PCR. TaCTR1 expression under stresses was analyzed using semi-quantitative RT-PCR and the effect of overexpression of TaCTR1 on salt tolerance was conducted in tobacco. The full-length cDNA of TaCTR1 is 2 635 bp which codes for a polypeptide of 759 amino acids. There is a conserved serine/threonine protein kinase domain at the carboxyl terminus containing an ATP-binding site. Southern blot analysis revealed that TaCTR1 consisted of a gene family in wheat. The amino acid homologies of CTR1 among different organisms share higher similarities. Expression analysis revealed that TaCTR1 was induced by NaCl and drought stress but inhibited by ABA treatment. Transient expression of TaCTR1-GFP in the onion epidermal cells indicated that TaCTR1 was probably targeted to the plasma membrane. Overexpression of TaCTR1 decreased salt tolerance in transgenic tobacco (Nicotiana tabacum L.) plants compared with the control. To our knowledge, TaCTR1 is the first CTR1 gene cloned in wheat and may be involved in various abiotic stresses. Overexpression of TaCTR1 decreased the salt tolerance in tobacco suggested that TaCTR1 may act as a negative regulator of salt stress in plants.

分类号：

  • 相关文献

[1]小麦类CTR1基因的克隆和特性分析. 秘彩莉,温小杰,张学勇,刘旭. 2009

[2]甘蔗乙烯信号转导途径关键基因CTR1的克隆与表达分析. 王凡伟,肖冬,李杨瑞,何龙飞,王爱勤. 2020

[3]Molecular cloning and characterization of a novel stress responsive gene in alfalfa. Sun, Y.,Long, R.,Yang, Q.,Kang, J.,Chao, Y.,Wu, M.,Long, R.,Wang, P.,Qin, Z.. 2012

[4]Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake. Pu, L.,Zhang, L. C.,Zhang, J. S.,Wang, L. G.,Liang, J.,Zhang, Y. B.,Liu, X.,Yan, H.,Zhang, T.,Yue, J. W.,Wang, L. X.,Song, X.,Li, N.,Wu, Q. Q.. 2016

[5]Cloning and characterization of gene encoding a Mn-containing superoxide dismutase in Eutrema halophilum. Xu, X. J.,Zhou, Y. J.,Bu, H. H.,Feng, J. C.,Ren, D. T.,Wang, G. Y.. 2014

[6]Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum. Chen Shuai,Liu Guan-shan,Wang Yuan-ying,Sun Yu-he,Chen Jia. 2011

[7]Molecular cloning and characterization of hatching enzyme-like gene in the silkworm, Bombyx mori. Tang, Shun-ming,Shen, Xing-jia,Zhao, Qiao-ling,Zhang, Guo-zheng,Guo, Xi-jie,Lu, Fu-hao,Wang, Na.

[8]Identification, bioinformatic analysis and expression profiling of candidate mRNA-like non-coding RNAs in Sus scrofa. Xiao, Bang,Zhang, Xingju,Li, Yong,Tang, Zhonglin,Yang, Shulin,Mu, Yulian,Cui, Wentao,Ao, Hong,Li, Kui,Xiao, Bang. 2009

[9]Gene cloning and sequencing of aminopeptidase N3, a putative receptor for Bacillus thuringiensis insecticidal Cry1Ac toxin in Helicoverpa armigera (Lepidoptera : Noctuidae). Wang, GR,Liang, GM,Wu, KM,Guo, YY. 2005

[10]Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters. Li, Jiaqi,Wang, Liangliang,Zhang, Hao,Chen, Songling,Mei, Yingjie,Wang, Chong,Ling, Fei,Du, HongLi,Fang, Wei,Chen, Yaosheng,Liu, Xiaohui. 2010

[11]Molecular cloning and bacterial expression of pheromone binding protein in the antennae of Helicoverpa armigera (Hubner). Wu, KM,Guo, YYN. 2004

[12]Cloning, expression and immunocytochemical localization of a general odorant-binding protein gene from Helicoverpa armigera (Hubner). Wang, GR,Wu, KM,Guo, YY. 2003

[13]Screening for Pea Germplasms Resistant to Fusarium Wilt Race 5. Deng, Dong,Sun, Suli,Wu, Wenqi,Zong, Xuxiao,Yang, Xiaoming,Zhang, Xiaoyan,He, Yuhua,Duan, Canxing,Zhu, Zhendong. 2022

[14]Functional Genomics and Comparative Lineage-Specific Region Analyses Reveal Novel Insights into Race Divergence in Verticillium dahliae. Wang, Dan,Zhang, Dan-Dan,Usami, Toshiyuki,Liu, Lei,Yang, Lin,Huang, Jin-Qun,Song, Jian,Li, Ran,Kong, Zhi-Qiang,Li, Jun-Jiao,Wang, Jun,Klosterman, Steven J.,Subbarao, Krishna, V,Dai, Xiao-Feng,Chen, Jie-Yin. 2021

[15]Functional Characterization of Verticillium dahliae Race 3-Specific Gene VdR3e in Virulence and Elicitation of Plant Immune Responses. Tan, Qian,Li, Ran,Liu, Lei,Wang, Dan,Dai, Xiao-Feng,Song, Li-Min,Zhang, Dan-Dan,Kong, Zhi-Qiang,Klosterman, Steve J.,Usami, Toshiyuki,Subbarao, Krishna V.,Liang, Wen-Xing,Chen, Jie-Yin. 2023

[16]Deletion of the 3′ End of the Introduced cry1Ac Gene Retains the Insecticidal Activity in Transgenic Cotton. Chunmeng Huang,Yongjun Zhang,Huilin Yu,Chenhui Hou,Haoyuan Guan,Xiuping Chen,Jiajian Xie. 2024

[17]Isolation and characterization of Brittle2 promoter from Zea Mays and its comparison with Ze19 promoter in transgenic tobacco plants. Chen, Xiaoping,Wang, Zhangying,Wang, Jianhua,Wang, Maoyan,Zhao, Li,Wang, Guoying. 2007

[18](E)-beta-Farnesene synthase genes affect aphid (Myzus persicae) infestation in tobacco (Nicotiana tabacum). Yu, Xiudao,Ma, Youzhi,Wang, Genping,Xu, Zhaoshi,Zhang, Baoming,Xia, Lanqin,Jones, Huw D.,Pickett, John A.,Zhang, Yongjun,Ren, Guangwei. 2012

[19]Expression of a nematode symbiotic bacterium-derived protease inhibitor protein in tobacco enhanced tolerance against Myzus persicae. Zhang, Heqing,Mao, Jianjun,Liu, Fengjiao,Zeng, Fanrong. 2012

[20]A Scutellaria baicalensis R2R3-MYB gene, SbMYB8, regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco. Yuan, Yuan,Qi, Linjie,Yang, Jian,Wu, Chong,Huang, Luqi,Liu, Yunjun.

作者其他论文 更多>>