数字农科院2.0

Application of ICP-AES to Detecting Nutrients in Grain of Wheat Core Collection of China

文献类型： 外文期刊

作者： Shi Rong-li;Zou Chun-qin;Rui Yu-kui;Xia Xiao-ping;Zhang Fu-suo;Zhang Xue-yong

作者机构：

关键词： ICP-AES;Human health;Nutrients;Wheat grain;Wheat mini-core collection

期刊名称： SPECTROSCOPY AND SPECTRAL ANALYSIS

ISSN： 1000-0593

年卷期： 2009 年 29 卷 4 期

页码：

收录情况： SCI

摘要： Deficiency of micronutrients, especially iron and zinc, has been a serious malnutrition problem worldwide in human health. Increasing Fe and Zn concentrations in grains by means of plant breeding is a sustainable, effective and important way to improve human mineral nutrition and health. However, little information on grain Fe and Zn concentrations in Chinese wheat genotypes is available. Therefore, to determine the nutrients status especially these of micronutrients in wheat grain is necessary and very useful. Two hundred sixty two genotypes were selected from the wheat mini-core collections, which contained 23090 wheat genotypes in China and represented 72.2% of total genetic variation. All 262 genotypes were grown in soils of similar geographical and climate location in order to minimize the environmental effect. After harvesting, the grains were washed with deionized water and dried (around 70 degrees C), then digested in HNO(3) solution using a microwave accelerating reaction system (N/IARS). Nutrient concentrations in stock solution were analyzed by inductively Coupled plasma atomic emission spectrometry (ICP-AES). Remarkable genetic variations among grain nutrient concentrations (Fe, Mn, Cu, Zn, Mg, Ca, K and P) in the tested genotypes were detected. The concentrations of Fe, Zn, Mn, Cu, Ca, Mg, K and P in wheat grain were in the ranges of 34. 2-61. 2, 26.3-76. 0, 20. 9-56. 7, 3. 4-9. 8, 290-976, 1 129-2 210 mg.kg(-1); 0.34%-0. 85% and 0.296%-0. 580%, respectively. The corresponding average values were 45.1, 50.2, 37.9, 6.5, 51.5, 1.772 mg.kg(-1), 0.55% and 0.451%, respectively. Significant positive correlations between micronutrients (Fe, Mn, Zn, and Cu) in wheat grains were detected, and the correlation coefficients were 0.395** (Fe and Mn), 0.424** (Fe and Zn), 0.574** (Fe and Cu), and 0.474** (Mn and Cu), respectively. However, no significant difference was found in grain nutrient concentrations between spring-wheat and winter-wheat genotypes. This study provides valuable and important information for breeding wheat genotypes which are enriched with minerals in grains, especially Fe and Zn.

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