数字农科院2.0

Cloning and Expression Level Analysis of Two BnaANT Candidate Genes in Brassica napus

文献类型: 外文期刊

作者: Chen Bo;Wang Han-zhong;Li Yun-chang;Yan Xiao-hong;Wang Li-jun;Wei Wen-hui;Wang Tai-xia

作者机构:

关键词: AINTEGUMENTA gene;Brassica napus;BnaANT gene;yield-related gene;quantitative real-time PCR

期刊名称: AGRICULTURAL SCIENCES IN CHINA

ISSN: 1671-2927

年卷期: 2010 年 9 卷 4 期

页码:

收录情况: SCI

摘要: AINTEGUMENTA (ANT) gene has been proved to have a novel function on controlling the organ size and the seed weight in Arabidopsis thaliana, the research on its orthologous gene in Brassica napus will be of potential interest to elucidate the molecular mechanism of rapeseed development and increase the rapeseed output. A SMART cDNA library of the flower bud from B napus cultivar Zhongshuang 9 was constructed, and the full-length cDNAs of two homologous BnaANT candidate genes were cloned by PCR in B napus, namely BnaX.ANT.a (GenBank accession no DQ211969) and BnaX ANT b (accession no DQ211970) They shared 87.1 and 83 4% amino acid identity with ANT of A thaliana, respectively. There is 87.4% amino acid identity between BnaX ANT a and BnaX.ANT.b. By quantitative real-time PCR, the obviously differential expression levels of the two BnaANT candidate genes were detected in the 28 DAF seeds of the big and small grains B napus lines, the expression abundance of BnaANT in the 28 DAF seed of 9311 was over three times compared to that of 9260, which indicates that these two BnaANT genes are also likely related to the floral organ size and the seed weight in B napus

分类号:

  • 相关文献

[1]Gene expression profiling in the thiamethoxam resistant and susceptible B-biotype sweetpotato whitefly, Bemisia tabaci. Zhou, Xu-guo,Xie, Wen,Yang, Xin,Wang, Shao-li,Wu, Qing-jun,Yang, Ni-na,Li, Ru-mei,Jiao, Xiao-guo,Pan, Hui-peng,Liu, Bai-ming,Feng, Yun-tao,Xu, Bao-yun,Zhang, You-jun. 2012

[2]Reference genes for quantitative real-time PCR analysis and quantitative expression of P5CS in Agropyron mongolicum under drought stress. Tian Qing-song,Du Jian-cai,Han Bing,Wang Shu-yan,Wu Zhi-juan,Li Xiao-quan,Han Bing. 2016

[3]Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus. Zhang, Shu-zhen,Wu, Su-ran,Yu, Nai-tong,Wang, Jian-hua,Liu, Zhi-xin,Kulye, Mahesh,Zeng, Hong-mei. 2012

[4]Comparative expression analysis in susceptible and resistant Gossypium hirsutum responding to Verticillium dahliae infection by cDNA-AFLP. Wang, Sheng-Zheng,Liu, Kai,Si, Ning,Qi, Fang-Jun,Jian, Gui-Liang.

[5]Genome-wide identification of the auxin response factor (ARF) gene family and expression analysis of its role associated with pistil development in Japanese apricot (Prunus mume Sieb. et Zucc). Gao, Zhihong,Huo, Ximei,Xu, Yanshuai,Shi, Ting,Ni, Zhaojun,Song, Juan,Sun, Hailong.

[6]Molecular identification and differential expression of sensory neuron membrane proteins in the antennae of the black cutworm moth Agrotis ipsilon. Yang, Ruo-Nan,Guo, Meng-Bo,Wang, Gui-Rong,Wu, Kong-Ming,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhou, Jing-Jiang.

[7]Copy number variation detection using SNP genotyping arrays in three Chinese pig breeds. Dong, K.,Pu, Y.,Yao, N.,Shu, G.,Liu, X.,He, X.,Zhao, Q.,Guan, W.,Ma, Y..

[8]Assessment of transgene copy number and zygosity of transgenic maize overexpressing Cry1Ie gene with SYBRA (R) Green qRT-PCR. Zhang, Yuwen,Wang, Guoying,Liu, Yunjun,Zhang, Yuwen,Liu, Yan,Zhang, Jie,Wang, Jianhua.

[9]Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz. Yi, Shanjun,Qian, Yongqiang,Han, Lei,Sun, Zhenyuan,Liu, Junxiang,Ju, Guansheng,Fan, Chengming.

[10]Differentially expressed genes in the midgut of Silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Li, Mu-Wang,Wang, Xiu-Ye,Liu, Ting,Qin, Guang-Xing,Guo, Xi-Jie,Wang, Xiu,Zhao, Pan,Guo, Xi-Jie. 2009

[11]Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori. Wu, Ping,Li, Mu-Wang,Guo, Xi-Jie,Jiang, Yun-Feng,Wang, Zi-Sheng,Guo, Xi-Jie. 2010

[12]Molecular Characterization, Tissue, and Developmental Expression Profiles of MagR and Cryptochrome Genes in Agrotis ipsilon (Lepidoptera: Noctuidae). Hou, Youming,Chang, Hong,Fu, Xiaowei,Zhao, Shengyuan,He, Limei,Wu, Kongming.

[13]The genes involved in the protective effects of phytohormones in response to Verticillium dahliae infection in Gossypium hirsutum. Zhang, Wenwei,Jian, Guiliang,Qi, Fangjun,Si, Ning.

[14]Collaborative Validation of an Event-Specific Quantitative Real-Time PCR Method for Genetically Modified Rice Event TT51-1 Detection. Wu, Yuhua,Cao, Yinglong,Wu, Gang,Yang, Litao,Zhang, Dabing,Song, Guiwen,Shen, Ping.

[15]Expression profiles of growth hormone receptor and insulin-like growth factor I in cattle and yak tissues revealed by quantitative real-time PCR. Pei, Jie,Lang, Xia,Guo, Xian,Bao, Pengjia,Liang, Chunnian,Chu, Min,Feng, Ruilin,Yan, Ping,Pei, Jie,Lang, Xia,Guo, Xian,Bao, Pengjia,Liang, Chunnian,Chu, Min,Yan, Ping. 2013

[16]Identification and characterization of (E)-beta-caryophyllene synthase and alpha/beta-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton. Xiao, Yutao,Zhang, Wanna,Wu, Juan,Guo, Yuyuan,Zhang, Yongjun,Huang, Xinzheng,Wu, Junxiang,Koellner, Tobias G.. 2013

[17]Rapid and sensitive screening and identification of CRISPR/Cas9 edited rice plants using quantitative real-time PCR coupled with high resolution melting analysis. Yuan, Zheng,Cui, Jinjie,Zhang, Xiujie,Yang, Litao,Yang, Litao,Ba, Yan,Li, Rong,Zhang, Dabing,Song, Yu. 2020

[18]Quantitative Distribution and Transmission of Tea Plant Necrotic Ring Blotch Virus in Camellia sinensis. Ren, Hengze,Chen, Yao,Zhao, Fumei,Ding, Changqing,Zhang, Kexin,Wang, Lu,Yang, Yajun,Hao, Xinyuan,Wang, Xinchao. 2022

[19]A droplet digital PCR assay for detection and quantification of Verticillium nonalfalfae and V. albo-atrum. Di Wang,Enliang Liu,Haiyang Liu,Xi Jin,Chunyan Niu,Yunhua Gao,Xiaofeng Su. 2023

[20]Genome-Wide Exploration And Characterization Of M.ir172/Euap2 Genes In Brassica N apus L. For Likely Role In Flower Organ Development. Gao, Yumin,Ping, Xiaoke,Tan, Yingchao,Wang, Jia,Xu, Xinfu,Wang, Tengyue,Lu, Kun,Cao, Yanru,Jian, Hongju,Liu, Liezhao. 2019

作者其他论文 更多>>