Generation of infectious and pathogenic duck hepatitis virus type 1 from cloned full-length cDNA
文献类型： 外文期刊
作者： Liu, G. Q.;Yun, T.;Zhang, Y. M.;Yun, T.;Ni, Z.;Yu, B.;Chen, L.;Huang, J. G.;Chen, J. P.
作者机构：
关键词： RNA viruses;Picornaviruses;Infectious cDNA;Duck hepatitis virus type 1
期刊名称： VIRUS RESEARCH
ISSN： 0168-1702
年卷期： 2010 年 147 卷 2 期
页码：
收录情况： SCI
摘要： A full-length cDNA clone of duck hepatitis virus type 1 (DHV-1) isolated from duck was assembled in the plasmid vector pBluescript II SK+. RNA synthesized in vitro by means of a Sp6 promoter inserted in front of the cDNA produced infectious particles after transfection of BHK-21 cells, as shown by the appearance of cytopathic effect. The rescued virus was also found to be highly pathogenic to young ducks by intradermal injection and resulted in a fatal disease indistinguishable from that of wild-type virus. The availability of the first cDNA clone of DHV-1 will allow examination of the molecular mechanisms behind DHV-1 virulence and attenuation, which could in turn lead to the production of second-generation, genetically engineered DHV-1 vaccines. (C) 2009 Elsevier B.V. All rights reserved.
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