数字农科院2.0

The Structure and Sequence Analysis of TLR4 Gene in Cattle

文献类型： 外文期刊

作者： Xu Shang-zhong;Gao Xue;Li Jun-ya;Ren Hong-yan;Chen Jin-bao;Wang Xing-ping;Luoreng Zhuo-ma

作者机构：

关键词： cattle;toll-like receptor 4;LRR domain;TIR domain;DNA binding site

期刊名称： AGRICULTURAL SCIENCES IN CHINA

ISSN： 1671-2927

年卷期： 2009 年 8 卷 5 期

页码：

收录情况： SCI

摘要： Toll-like receptor 4 (TLR4) is essential for initiating the innate response to lipopolysaccharide (LPS) from Gram-negative bacteria by acting as a signal transducting receptor. In order to help in investigating TLR4 as a candidate disease-resistance gene in cows, we isolated the cDNA (GenBank accession no. DQ839566) by RT-PCR and rapid amplification of cDNA ends (RACE) experiments and analyzed the sequence characters by bioinformatics. The results showed that cattle TLR4 gene about 3 739 bp contains an open reading frame of 2 526 bp encoded 841 amino acids (aa), 470 bp 5' untranslated region (UTR), and 743 bp 3' UTR. Tissue expression profile by RT-PCR indicated that TLR4 gene expresses in mammary glands, liver, muscle, duodenum, fats, uterus, kidneys, hearts, lungs, pancreas, and ovary. TLR4 protein domain predicted by bioinformatics consists of signal peptide, transmembrane helices domain, 3 sorts of leucine-rich repeat domains (LRR, LRR-TYP, and LRRCT), and a toll-interleukin1-resistance domain (TIR). Leucine-rich repeat domains were related with recognizing a broad of pathogen-associated molecular patterns (PAMP) from pathogen, and TIR domain for downstream signaling transduction was most conservative (98% identify) than other domains after alignment of protein from ovine, porcine, human, and mouse. In addition, a 470 bp 5'-flanking region sequence was amplified by PCR, and 15 putative DNA binding sites were predicted, but this sequence lacks TATA box, CCAAT character, and GC-rich regions.

分类号：

  • 相关文献

[1]Haplotype analysis of TLR4 gene and its effects on milk somatic cell score in Chinese commercial cattle. Wang, Xing Ping,Zhuo Ma Luoreng,Guo, Dong Sheng,Li, Feng,Chen, Gang,Wang, Jin Ren,Gao, Shu Xin,Li, Jun Ya,Gao, Xue,Xu, Shang Zhong.

[2]Glycyrrhetinic acid extracted from Glycyrrhiza uralensis Fisch induces the expression of Toll-like receptor 4 in Ana-1 murine macrophages. Li, Li,Miao, Jian-Hua,Xu, Yong-Li,Peng, Li-Na,Qiu, Ya-Feng,Gao, Xiao-Qi,Shi, Zi-Xue,Shao, Dong-Hua,Wei, Jian-Chao,Ma, Zhi-Yong,Zhou, Ying. 2011

[3]Effects of dietary administering chitosan on growth performance, jejunal morphology, jejunal mucosal sIgA, occluding, claudin-1 and TLR4 expression in weaned piglets challenged by enterotoxigenic Escherichia coli. Xiao, Dingfu,Zhang, Bin,Xiao, Dingfu,Yin, Yulong,Feng, Zemeng,Tang, Zhiru,Hu, Xionggui,Wang, Jinquan. 2013

[4]Lovastatin Inhibits Toll-Like Receptor 4 S.ignaling In Microglia By T argeting Its Co-Receptor Myeloid Differentiation Protein 2 And Attenuates Neuropathic Pain. Peng, YH, Zhang, XZ, Zhang, TS, Grace, PM, Li, HY, Wang, YB, Li, H, Chen, HQ, Watkins, LR, Hutchinson, MR, Yin, H, Wang, XH. 2019

[5]ACT001 Inhibits TLR4 Signaling by Targeting Co-Receptor MD2 and Attenuates Neuropathic Pain. Zhang, Tianshu,Lin, Cong,Wu, Siru,Jin, Sha,Li, Xiaodong,Peng, Yinghua,Wang, Xiaohui. 2022

[6]In vivo transcription of bovine leukemia virus and bovine immunodeficiency-like virus. Wu, DL,Murakami, K,Morooka, A,Jin, H,Inoshima, Y,Sentsui, H. 2003

[7]Genetic heterogeneity among bovine leukemia virus genotypes and its relation to humoral responses in hosts. Licursi, M,Inoshima, Y,Wu, DL,Yokoyama, T,Gonzalez, ET,Sentsui, H. 2002

[8]A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle. Wang, Shuai,Wang, Zongli,Wang, Shuai,Hao, Haisheng,Zhao, Xueming,Zhu, Huabin,Du, Weihua,Wang, Dong,Liu, Yan,Qin, Tong,Wang, Shuai. 2012

[9]Genomic Imprinting of GTL2 in Somatic Cell Nuclear Transfer Cattle. Wang Jian-Chun,Fan Bao-Liang,Li Shi-Jie,Li Ning,Li Shi-Jie,Li Dong-Jie,Du Wei-Hua. 2008

[10]Characterization and mapping of the bovine FBP1 gene. Guo, H.,Liu, W-S.,Takasuga, A.,Eyer, K.,Landrito, E.,Xu, S-Z.,Gao, X.,Ren, H-Y.. 2007

[11]Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers. Zhang, L. P.,Gao, H. J.,Li, J. Y.,Xu, S. Z.,Gan, Q. F.,Hou, G. Y.. 2015

[12]Identification of differentially expressed proteins between bull X and Y spermatozoa. Chen, Xiaoli,Zhu, Huabin,Hao, Haisheng,Zhao, Xueming,Du, Weihua,Qin, Tong,Liu, Yan,Wang, Dong,Chen, Xiaoli,Wu, Chengjiang,Han, Weidong. 2012

[13]Association of Calpain 1 (CAPN1) and HRSP12 allelic variants in beef cattle with carcass traits. Hou, Guanyu,Huang, Meng,Gao, Xue,Li, Junya,Gao, Huijiang,Ren, Hongyan,Xu, Shangzhong,Hou, Guanyu. 2011

[14]Tissue expression and predicted protein structures of the bovine ANGPTL3 and association of novel SNPs with growth and meat quality traits. Chen, N. B.,Ma, Y.,Fu, W. W.,Xu, Y. J.,Li, F.,Yang, T.,Lin, F.,Li, J. Y.,Gao, S. X..

[15]An isolation, polymorphism and trait association study of bovine GAD1 and GAL genes. Guo, H.,Li, K.,Liu, W-S.,Li, H-D.,Xu, S-Z.,Ren, H-Y.,Gao, X.. 2006

[16]Association of LXRA gene variants with carcass and meat quality traits in beef cattle. Gan, Qianfu,Xu, Shangzhong,Gan, Qianfu,Zhang, Lupei,Xu, Shangzhong,Li, Hengde,Li, Junya,Gao, Xue,Ren, Hongyan,Chen, Jinbao. 2008

[17]Phylogenetic analysis of the bovine parainfluenza virus type 3 from cattle herds revealing the existence of a genotype A strain in China. Wen, Yong-Jun,Shi, Xin-Chuan,Wang, Feng-Xue,Wang, Wei,Zhang, Shu-Qin,Li, Guo,Song, Ni,Chen, Li-Zhi,Cheng, Shi-Peng,Wu, Hua. 2012

[18]Mixed Nipple Infections Caused by Variant of BPV3 and a Putative New Subtype of BPV in Cattle. He, Z.,Meng, Q.,Qiao, J.,Peng, Y.,Xie, K.,Liu, Y.,Chen, C.,Cai, X.,Zhang, J..

[19]PPAR alpha signal pathway gene expression is associated with fatty acid content in yak and cattle longissimus dorsi muscle. Qin, W.,Liang, C. N.,Guo, X.,Chu, M.,Pei, J.,Bao, P. J.,Wu, X. Y.,Li, T. K.,Yan, P.. 2015

[20]Residues of Salbutamol and Identification of Its Metabolites in Beef Cattle. Zhang, Kai,Tang, Chaohua,Meng, Qingshi,Zhao, Qingyu,Liang, Xiaowei,Liu, Shengsheng,Zhang, Junmin,Du, Wei,Bo, Tao,Zhang, Zhixu.

作者其他论文 更多>>