数字农科院2.0

The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo

文献类型： 外文期刊

作者： Bai Li-jing;Ju Hui-ming;Mu Yu-Lian;Yang Shu-lin;Ren Hong-yan;Ao Hong;Li Kui;Bai Li-jing;Wang Chu-duan;Ju Hui-ming

作者机构：

关键词： type A spermatogonial stem cells;dicistronic expression vector;co-expression;transgenic mice

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2014 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coli appA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the first time. The dicistronic expression vector pcDNA-appA-MxA(AMP) and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testis-mediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. Transgenic mice that co-expressed appA and MxA showed a gene integration rate of 8.89% (16/180). The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1% by the appA gene (P<0.05); these animals also exhibited a strong anti-viral phenotype.

分类号：

  • 相关文献

[1]Study of The Transgenic Efficiency in Different Spermiogenesis Stages in Mice. Zhang Xin,Miao Xiang-Yang,Qu Zhao-Jie,Zhang Qiu-Ting,Zhang Xin,Yin Xun-He,Ma Yan-Fang,Qu Zhao-Jie. 2009

[2]Muscle hypertrophy in transgenic mice due to over-expression of porcine myostatin mutated at its cleavage site. Qian Li-li,Cai Chun-bo,An Xiao-rong,Ma De-zun,Gao Peng-fei,Jiang Sheng-wang,Wang Qing-qing,Xiao Gao-jun,Cui Wen-tao. 2016

[3]Production of transgenic mice carrying the Thanatin gene by intratesticular injection. Miao, Xiang-Yang,Zhang, Xin. 2011

[4]Functional verification of a porcine myostatin propeptide mutant. Ma, Dezun,Jiang, Shengwang,Gao, Pengfei,Wang, Qingqing,Xiao, Gaojun,Cui, Wentao,Qian, Lili,Cai, Chunbo,Yang, Jinzeng.

[5]Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice. Ma, Dezun,Gao, Pengfei,Wang, Qingqing,Jiang, Shengwang,Xiao, Gaojun,Cui, Wentao,Qian, Lili,Cai, Chunbo. 2015

[6]Transgenic mice with overexpression of mutated human optineurin(E50K) in the retina. Meng, Qingfeng,Xiao, Zheng,Yuan, Huiping,Zhou, Xinrong,Yang, Binbin,Sun, Jingbo,Meng, Bo,Sun, Xian,Cheng, Fang,Xue, Fei,Zhu, Yuanmao.

[7]Production of Transgenic Mice by Type-A Spermatogonia-Mediated Gene Transfer. Ju Hui-ming,Bai Li-jing,Ren Hong-yan,Mu Yu-lian,Yang Shu-lin,Li Kui,Ju Hui-ming. 2011

[8]Skin-specific transgenic overexpression of ovine β-catenin in mice. Jiankui Wang,Kai Cui,Guoying Hua,Deping Han,Zu Yang,Tun Li,Xue Yang,Yuanyuan Zhang,Ganxian Cai,Xiaotian Deng,Xuemei Deng. 2023

[9]Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants. He, Zhimin,He, Reqing,Yan, Jindong,Zhong, Ming,Zhao, Xiaoying,Liu, Xuanming,He, Zhimin,Wang, Xu,Liu, Bin,Bian, Mingdi,Liu, Xuanming. 2016

[10]De novo transcriptome sequencing and gene expression profiling of Elymus nutans under cold stress. Fu, Juanjuan,Hu, Tianming,Yang, Peizhi,Miao, Yanjun,Shao, Linhui. 2016

[11]Construction and co-expression of polycistronic plasmids encoding bio-degumming-related enzymes to improve the degumming process of ramie fibres. Cheng, Yi,Liu, Zhengchu,Zeng, Jie,Cheng, Lifeng,Yan, Zhun,Duan, Shengwen,Feng, Xiangyuan,Zheng, Ke,Zheng, Xia,Wang, Ruijun.

[12]Characterization of a novel lipase and its specific foldase from Acinetobacter sp XMZ-26. Zheng, Xiaomei,Wu, Ningfeng,Fan, Yunliu. 2012

[13]Constructing A Comprehensive Gene Co-Expression B.ased Interactome In Bos T aurus. Chen, Y, Liu, YN, Du, M, Zhang, WG, Xu, L, Gao, X, Zhang, LP, Gao, HJ, Xu, LY, Li, JY, Zhao, M. 2017

[14]Comprehensive identification of polygalacturonases in cotton: Genomic analysis, potential regulatory mechanisms and expression patterns in anthers. Zhanshuai Li,Xiaocong Cao,Huiying Wang,Yongming Liu,Wenqian Liu,Huiyuan Zhang,Meng Kuang,Jun Peng. 2023

[15]Identification and analysis of lncRNA, miRNA and mRNA related to subcutaneous and intramuscular fat in Laiwu pigs. Feng H.,Liu T.,Yousuf S.,Zhang X.,Huang W.,Li A.,Xie L.,Miao X.. 2023

[16]Identification and Characterization of Long Non-coding RNA in Tomato Roots Under Salt Stress. Li N.,Wang Z.,Wang B.,Wang J.,Xu R.,Yang T.,Huang S.,Wang H.,Yu Q.. 2022

[17]Comparative transcriptomic analysis reveals region-specific expression patterns in different beef cuts. Zhang, Tianliu,Wang, Tianzhen,Niu, Qunhao,Zheng, Xu,Li, Haipeng,Gao, Xue,Chen, Yan,Gao, Huijiang,Zhang, Lupei,Liu, George E.,Li, Junya,Xu, Lingyang. 2022

[18]Comparative Transcriptome Analysis of Gayal (Bos frontalis), Yak (Bos grunniens), and Cattle (Bos taurus) Reveal the High-Altitude Adaptation. Jun Ma,Tianliu Zhang,Wenxiang Wang,Yan Chen,Wentao Cai,Bo Zhu,Lingyang Xu,Huijiang Gao,Lupei Zhang,Junya Li,Xue Gao. 2022

[19]Genome-wide analysis of HACD family genes and functional characterization of GhHACD2 for very long chain fatty acids biosynthesis in Gossypium hirsutum. Minwen Yan,Hongyuan Xi,Shiyin Hu,Jikun Song,Bing Jia,Pan Feng,Liupeng Yang,Jianjiang Ma,Li Wang,Wenfeng Pei,Bingbing Zhang,Jiwen Yu,Man Wu,Shoulin Hu. 2024

[20]Integration of transcriptome and metabolome reveals regulatory mechanisms of volatile flavor formation during tomato fruit ripening. Liu, Zhiqiang,Wu, Jianxin,Wang, Licai,Lu, Xiaonan,Ahammed, Golam Jalal,Zhang, Xiaolan,Cui, Xia,Wang, Haijing. 2025

作者其他论文 更多>>