数字农科院2.0

Identification of the miniature pig inbred line by skin allograft

文献类型： 外文期刊

作者： Mu Yu-lian;Liu Lan;Feng Shu-tang;Wu Tian-wen;Li Kui;He Wei;Gao Qian;Wei Jing-liang;Tang Fang;Yang Shu-lin;Xia Ying;Li Jun-you;Zhou Wen-fang;Wu Zhi-gu;Sun Tong-zhu

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关键词： Wuzhishan miniature pigs;inbred line;skin allograft;immune rejection

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2015 年 14 卷 7 期

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收录情况： SCI

摘要： Skin grafting has been used as one of the most reliable tests to determine the genetic stability of laboratory animal such as mice and rats inbred line, but no identification of swine inbred lines by skin grafting has been reported. At present, Wuzhishan miniature pig (WZSP) inbred line has acquired the F-24 individuals in China. In order to verify whether WZSP inbred line had been cultivated successfully, allogeneic skin grafts and related research were performed on F-20 individuals of WZSP inbreeding population, compared with a control group of autologous transplantation. We observed the transplant recipients' wounds, detected peripheral blood-related indicators interleukin-2, 4 and 10, CD4(+) and CD8(+) lymphocytes, and conducted hematoxylin-eosin (HE) and Masson's staining of skin to judge whether the immune rejection reactions occurred within 28 days after transplantation. Chr. 7 genomic heterozygosity of 48 WZSP individuals from F-20 to F-22 was analyzed by high-density single nucleotide polymorphism (SNP) chips (60000 SNPs). The result showed that there were no significant differences in graft skin, the plasma interleukin-2, 4, 10, CD4(+) and CD8(+), HE and Masson's staining results between the allograft and autograft groups, and no immune rejection occurred on the allograft group. We found that 11 genes in Chr. 7 of major histocompatibility complex (MHC) I and MHC II were homozygous which confirmed that immune antibody of the allograft and autograft groups were highly identical and also provided a theoretical basis to no immune rejection occurred on the allograft in the inbred WZSP. The result proved that the WZSP inbred line had been cultivated successfully for the first time in the world. The test methods also provide a scientific basis for the identification of swine and mammal inbred lines.

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