数字农科院2.0

Pathogen-resistant transgenic plant from dwarfing rootstock of cherry by introducing antibacterial polypeptide genes

文献类型： 外文期刊

作者： Fang, HJ;Wang, GL;Wang, HX;Jia, SR;Dong, YZ;Tang, YX

作者机构：

关键词： cherry;antibacterial polypeptide gene;shoot transformation

期刊名称： ACTA BOTANICA SINICA

ISSN： 0577-7496

年卷期： 1999 年 41 卷 11 期

页码：

收录情况： SCI

摘要： A shoot regeneration system of high frequency from shoot of Chinese cherry (Prunus pseudocerasus Lindl.) dwarfing rootstock was established and 19 transformed plants were obtained from the dwarfing rootstock by introducing gus gene and antibacterial polypeptide genes, using Agrobacterium tumefaciens (Smith et Townsend) Conn as mediator. The results of Southern blot analysis proved that antibacterial polypeptide gene was integrated into the cherry genome. The results of tumor test, X-gluc reaction and bacterial-resistant test of leaf extract against Agrobacterium tumefaciens C-58 indicated that antibacterial polypeptide gene mag. he expressed efficiently in the transformed plant. The tube shoot was characteristic in its resistance to root nodule disease. This study on the characteristics of germ line transformation of shoot meristem suggested that it was a highly efficient transformation system.

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