数字农科院2.0

Identifying different types of dedifferentiated microspores from indica-japonica F-1 hybrids with subspecies-differentiating RFLP probes in rice

文献类型： 外文期刊

作者： Xie, JH;Lu, J;Zhuang, JY;Lin, HX;Qian, HR;Gao, MW;Zheng, KL

作者机构：

关键词： de-differentiated microspore;indica-japonica F-1, hybrid;restriction fragment length polymorphism (RFLP);subspecies-differentiating probe;Oryza sativa L

期刊名称： THEORETICAL AND APPLIED GENETICS

ISSN： 0040-5752

年卷期： 1997 年 94 卷 1 期

页码：

收录情况： SCI

摘要： The indica,japonica and intermediary types of de-differentiated microspores from indica-japonica F-1 hybrids were identified with 11 subspecies-differentiating RELP probes in rice (Oryza sativa L.). The results showed that the distribution of indica, japonica and intermediary types of de-differentiated microspores could be easily detected in a simple and quick way using the RFLP method. Moreover, the microspores from the same F-1 hybrid but inoculated onto different media, or microspores from different F-1 hybrids when inoculated onto the same medium, often displayed distinctive distribution curves of de-differentiated microspores types, indicating that the media employed in this experiment had high selectivity for the de-differentiation of certain types of microspores. The application of the RELP method to de-differentiated microspore identification is of great theoretical and practical significance in rice doubled-haploid breeding.

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