数字农科院2.0

Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.)

文献类型： 外文期刊

作者： Liu, Hongyan;Zhou, Fang;Yang, Minmin;Zhou, Ting;Zhao, Yingzhong;Tan, Mingpu;Yu, Haijuan;Li, Liang

作者机构：

关键词： Sesame;Dominant genic male sterile;Transcriptome;Differentially expressed genes

期刊名称： BMC PLANT BIOLOGY

ISSN： 1471-2229

年卷期： 2016 年 16 卷

页码：

收录情况： SCI

摘要： Background: Sesame (Sesamum indicum L.) is a globally important oilseed crop with highly-valued oil. Strong hybrid vigor is frequently observed within this crop, which can be exploited by the means of genic male sterility (GMS). We have previously developed a dominant GMS (DGMS) line W1098A that has great potential for the breeding of F-1 hybrids. Although it has been genetically and anatomically characterized, the underlying molecular mechanism for male sterility remains unclear and therefore limits the full utilization of such GMS line. In this study, RNA-seq based transcriptome profiling was carried out in two near-isogenic DGMS lines (W1098A and its fertile counterpart, W1098B) to identify differentially expressed genes (DEGs) related to male sterility. Results: A total of 1,502 significant DEGs were detected, among which 751 were up-regulated and 751 were down-regulated in sterile flower buds. A number of DEGs were implicated in both ethylene and JA synthesis & signaling pathway; the expression of which were either up-or down-regulated in the sterile buds, respectively. Moreover, the majority of NAC and WRKY transcription factors implicated from the DEGs were up-regulated in sterile buds. By querying the Plant Male Reproduction Database, 49 sesame homologous genes were obtained; several of these encode transcription factors (bHLH089, MYB99, and AMS) that showed reduced expression in sterile buds, thus implying the possible role in specifying or determining tapetal fate and development. The predicted effect of allelic variants on the function of their corresponding DEGs highlighted several Insertions/Deletions (InDels), which might be responsible for the phenotype of sterility/fertility in DGMS lines. Conclusion: The present comparative transcriptome study suggested that both hormone signaling pathway and transcription factors control the male sterility of DGMS in sesame. The results also revealed that several InDels located in DEGs prone to cause loss of function, which might contribute to male sterility. These findings provide valuable genomic resources for a deeper insight into the molecular mechanism underlying DGMS.

分类号：

  • 相关数据
  • 相关文献

[1]Additional file 1: Table S2. of Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Yingzhong Zhao,Zhou, Fang,Zhou, Ting,Mingpu Tan,Haijuan Yu,Hongyan Liu,Li, Liang,Minmin Yang

[2]Additional file 8: Table S1. of Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Yingzhong Zhao,Mingpu Tan,Zhou, Ting,Haijuan Yu,Minmin Yang,Hongyan Liu,Zhou, Fang,Li, Liang

[3]Additional file 5: Table S6. of Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Mingpu Tan,Zhou, Ting,Yingzhong Zhao,Haijuan Yu,Minmin Yang,Zhou, Fang,Li, Liang,Hongyan Liu

[4]Additional file 6: Table S7. of Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Minmin Yang,Zhou, Ting,Yingzhong Zhao,Zhou, Fang,Mingpu Tan,Li, Liang,Hongyan Liu,Haijuan Yu

[5]Additional file 2: Table S3. of Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Li, Liang,Zhou, Ting,Mingpu Tan,Hongyan Liu,Yingzhong Zhao,Haijuan Yu,Zhou, Fang,Minmin Yang

[6]Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Zhou, Ting,Yingzhong Zhao,Zhou, Fang,Hongyan Liu,Haijuan Yu,Minmin Yang,Mingpu Tan,Li, Liang

[7]Additional file 4: Table S5. of Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Yingzhong Zhao,Mingpu Tan,Hongyan Liu,Zhou, Fang,Li, Liang,Zhou, Ting,Minmin Yang,Haijuan Yu

[8]Additional file 7: Table S8. of Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Yingzhong Zhao,Zhou, Ting,Mingpu Tan,Zhou, Fang,Minmin Yang,Hongyan Liu,Li, Liang,Haijuan Yu

[9]Additional file 3: Table S4. of Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Yingzhong Zhao,Haijuan Yu,Zhou, Ting,Li, Liang,Zhou, Fang,Mingpu Tan,Minmin Yang,Hongyan Liu

[1]De novo transcriptomic analysis of the female and male adults of the blood fluke Schistosoma turkestanicum. Chang, Qiao-Cheng,Wang, Chun-Ren,Liu, Guo-Hua,Xu, Min-Jun,Zhu, Xing-Quan,Xu, Min-Jun,Gao, Jun-Feng,Zhu, Xing-Quan. 2016

[2]Genome-wide transcriptional changes of ramie (Boehmeria nivea L. Gaud) in response to root-lesion nematode infection. Tang, Shouwei.

[3]De novo assembly and transcriptome analysis of two contrary tillering mutants to learn the mechanisms of tillers outgrowth in switchgrass (Panicum virgatum L.). Kaijie Xu,Fengli Sun,Guaiqiang Chai,Yongfeng Wang,Lili Shi,Shudong Liu,Yajun Xi. 2015

[4]Comparative Transcriptome Profile of the Cytoplasmic Male Sterile and Fertile Floral Buds of Radish (Raphanus sativus L.). Mei, Shiyong,Liu, Touming,Liu, Touming,Wang, Zhiwei. 2016

[5]Transcriptome profiling of maize (Zea mays l.) leaves reveals key cold-responsive genes, transcription factors, and metabolic pathways regulating cold stress tolerance at the seedling stage. Joram Kiriga Waititu,Quan Cai,Ying Sun,Yinglu Sun,Congcong Li,Chunyi Zhang,Jun Liu,Huan Wang. 2021

[6]Comparative Transcriptomic Analysis Reveals Diverse Expression Pattern Underlying Fatty Acid Composition among Different Beef Cuts. Tianliu Zhang,Qunhao Niu,Tianzhen Wang,Xu Zheng,Haipeng Li,Xue Gao,Yan Chen,Huijiang Gao,Lupei Zhang,George E. Liu,Junya Li,Lingyang Xu. 2022

[7]Transcriptome profiling of maize (Zea mays l.) leaves reveals key cold-responsive genes, transcription factors, and metabolic pathways regulating cold stress tolerance at the seedling stage. Joram Kiriga Waititu,Quan Cai,Ying Sun,Yinglu Sun,Congcong Li,Chunyi Zhang,Jun Liu,Huan Wang. 2021

[8]Knockdown of CYP9A9 increases the susceptibility to lufenuron, methoxyfenozide and a mixture of both in Spodoptera exigua. Zhang, Zhixian,Wang, Dan,Shan, Yongpan,Chen, Jixiang,Hu, Hongyan,Song, Xianpeng,Ma, Xiaoyan,Ren, Xiangliang,Ma, Yan. 2022

[9]Gene Expression Profiling of African Bermudagrass under Cold Acclimation. Felipe Cevallos,Mingying Xiang,Xiaoyu Zhang,Yanqi Wu,Justin Moss,Charles Fontanier,Dennis Martin,Shuhao Yu. 2025

[10]Comparative Transcriptome Analysis of Cold Tolerance Mechanism in Honeybees (Apis mellifera sinisxinyuan). Jinqiong Shan,Ruiyi Cheng,Tuohudasheng Magaoya,Yujie Duan,Chao Chen. 2024

[11]Transcriptomic And Metabolomic Profiling Of D.rought-Tolerant And Susceptible Sesame G enotypes In Response To Drought Stress. Zhang, Yujuan,Dossa, Komivi,Zhang, Xiurong,Yu, Jingyin,Zhang, Yanxin,Zhou, Rong,Zhang, Yujuan,Liu, Aili,Li, Donghua,Wang, Linhai,You, Jun,Wang, Xiao,Dossa, Komivi. 2019

[12]Genome-Wide Characterization And Expression Analysis O.f The Hd-Zip Gene F amily In Response To Drought And Salinity Stresses In Sesame. Liu, Aili,You, Jun,Zhang, Yujuan,Zhou, Yong,Zhang, Yujuan,Dossa, Komivi,Wei, Mengyuan,Zhou, Rong,Li, Donghua,Zhang, Xiurong. 2019

[13]Genome-Wide Association Studies Of 39 S.eed Yield-Related Traits In S esame (Sesamum Indicum L.). Dossa, Komivi,You, Jun,Wei, Xin,Zhang, Xiurong,Zhou, Rong,Dossa, Komivi,Yu, Jingyin,Li, Donghua,Wei, Xin. 2018

[14]Insight Into The Evolution And F.unctional Characteristics Of The P an-Genome Assembly From Sesame Landraces And Modern Cultivars. Dossa, Komivi,Yu, Jingyin,Lu, Kun,Chen, Jinfeng,Dossa, Komivi,Edwards, David,Edwards, David,Lu, Kun,Zhang, Xiurong,Zhang, Yanxin,You, Jun,Wang, Linhai,Golicz, Agnieszka A.,Fan, Dingding. 2019

[15]Inheritance and molecular mapping of a novel dominant genic male-sterile gene in Sesamum indicum L.. Liu, Hongyan,Zhou, Xinan,Wu, Kun,Yang, Minmin,Zhao, Yingzhong.

[16]Integrated Small Rna And Degradome Sequencing Provide Insights Into Salt Tolerance In Sesame (Sesamum Indicuml.). Zhao, Fengtao,Gong, Huihui,Zhang, Xiurong,Zhou, Rong,Zhang, Yujuan,Zhang, Yujuan,You, Jun,Li, Donghua. 2020

[17]Development, inheritance and breeding potential of a recessive genic male sterile line D248A in Sesame (Sesamum indicum L.). Liu, Hongyan,Yang, Minmin,Wu, Kun,Zhou, Xinan,Zhao, Yingzhong. 2013

[18]Identification of Sesame Genomic Variations from Genome Comparison of Landrace and Variety. Wei, Xin,Zhu, Xiaodong,Yu, Jingyin,Wang, Linhai,Zhang, Yanxin,Li, Donghua,Zhou, Rong,Zhang, Xiurong. 2016

[19]Morpho-anatomical and physiological responses to waterlogging of sesame (Sesamum indicum L.). Wei, Wenliang,Li, Donghua,Wang, Linhai,Ding, Xia,Zhang, Yanxin,Gao, Yuan,Zhang, Xiurong. 2013

[20]Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers. Zhang, Yan-xin,Zhang, Xiu-rong,Hua, Wei,Wang, Lin-han,Che, Zhuo. 2010

作者其他论文 更多>>