数字农科院2.0

Monospecific and ultrasensitive detection of ofloxacin: A computational chemistry-assisted hapten screening strategy and analysis of molecular recognition mechanism

文献类型： 外文期刊

作者： Xiao J.;Qin L.;Zhao D.;Huang N.;Xu W.;Zhang L.;Pan X.;Han S.;Ding M.;Li L.;Le T.;Peng D.

作者机构：

关键词： （0-1-4）Computational simulation;Hapten screening;Molecular mechanism;Monospecificity;Ofloxacin;Residue detection

期刊名称： Journal of Hazardous Materials

ISSN： 0304-3894

年卷期： 2024 年 465 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Contamination in food and the environment with fluoroquinolones (FQs) has become a serious threat to the global ecological balance and public health safety. Ofloxacin (OFL) is one of the most widely utilized sterilization agents in FQs. In the process of monitoring OFL, broad-spectrum monoclonal antibodies (mAb) cannot meet the demand for monospecific detection. Here, a computational chemistry-assisted hapten screening strategy was proposed in this study. Differences in the properties of antigenic epitopes were precisely extracted through a comprehensive comparative study of 16 common FQs molecules and a monospecific and ultrasensitive mAb-3B4 for OFL was successfully prepared. The screened fleroxacin (FLE) hapten was applied in a heterologous competition strategy resulting in a 20-fold improvement in the half inhibitory concentration (IC50) of mAb-3B4 to 0.0375 μg L−1 and cross-reacted only with marbofloxacin (MAR) in regulated FQs. In addition, a single-chain variable fragment (scFv) for OFL was constructed for the first time with an IC50 of 0.378 μg L−1. Molecular recognition mechanism studies validated the reliability of this strategy and revealed the key amino acid sites responsible for OFL specificity and sensitivity. Finally, ic-ELISA and GICA were established for OFL in real samples. This work provides new ideas for the preparation of monospecific mAb and improves the monitoring system of FQs. © 2023 Elsevier B.V.

分类号：

  • 相关文献

[1]High efficient PMS activation by synergistic effects of bimetallic sulfide FeS2@MoS2 for rapid OFX degradation. Juan Tang,Jin Xu,Hengrui Zhang,Wen Liu,Hongna Li,Jianxin Xia,Xuan Xing. 2023

[2]Anti-oxytetracycline monoclonal antibody based detection of oxytetracycline residue in honey. Xu, Shufa,Hou, Liwei,Wubie, Abebe J.,Cao, Tan,Hu, Yingying,Li, Wei,Guo, Zhanbao,Zhao, Jing,Wu, Jie,Hou, Liwei,Zhao, Jing. 2013

[3]Dual-mode detection of organophosphate pesticides in pear and Chinese cabbage based on fluorescence and AuNPs colorimetric assays. He Yu,Miao Wang,Jing Cao,Yongxin She,Yongan Zhu,Jiaming Ye,A. M. Abd El-Aty,Ahmet Hacımüftüoğlu,Jing Wang,Shuibing Lao. 2021

[4]Highly Sensitive Magnetic-Nanoparticle-Based Immunochromatography Assay for Rapid Detection of Amantadine in Chicken and Eggs. Li H.,Lu Y.,Zhang L.,Qin L.,Wen H.,Fan X.,Peng D.. 2024

[5]A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment. Zhang L.,Yin X.,Yang H.,Wen H.,Han S.,Pan X.,Li H.,Peng D.. 2024

[6]Rapid immunoassays for the detection of quinoxalines and their metabolites residues in animal-derived foods: A review. Liangni Qin,Jiaxu Xiao,Hongfei Yang,Jixiang Liang,Long Li,Shixiang Wu,Dapeng Peng. 2024

[7]Simultaneous Determination of Sodium Pentachlorophenolate and Its Metabolites in Swine Samples. Qi Jia,Miao Cui,Meiling Li,Yuncheng Li,Fanbing Meng,Yanyang Xu,Yongzhong Qian,Xue Yan,Jing Qiu. 2023

[8]Development of a Highly Sensitive Time-Resolved Fluorescence Immunochromatographic Assay for Rapid Onsite Determination of Prometryn Residues in Food Samples. Zhao, Jing,Gao, Song,Li, Peipei,Abd El-Aty, A. M.,Xu, Lingyuan,Jin, Fen,Wang, Jing,Li, Jia,Lin, Weiping,Zheng, Lufei,Jiao, Bining. 2026

[9]Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize. Li, Zhao,Hu, Guanghui,Liu, Xiangfeng,Zhou, Yao,Zhang, Qian,Yang, Deguang,Zhang, Zhiwu,Li, Zhao,Hu, Guanghui,Zhang, Xu,Yuan, Xiaohui,Zhang, Zhiwu,Hu, Guanghui,Wang, Tianyu,Yuan, Xiaohui. 2016

[10]Gibberellin-induced mesocotyl elongation in deep-sowing tolerant maize inbred line 3681-4. Zhao, G.,Ma, P.,Wu, L.,Wang, J.,Zhao, G.,Ma, P.,Wu, L.,Wang, J.,Zhao, G.,Fu, J.,Wang, G..

[11]Cloning and phylogenetic analysis of polyphenol oxidase genes in common wheat and related species. He, X. Y.,He, Z. H.,Xia, X. C.,He, Z. H.,Morris, C. F..

[12]Differential Gene Expression Between Hybrids and Their Parents During the Four Crucial Stages of Cotton Growth and Development. Zhao Yun-lei,Yu Shu-xun,Xing Chao-zhu,Fan Shu-li,Song Mei-zhen,Ye Wu-wei. 2009

[13]PeaT1-induced systemic acquired resistance in tobacco follows salicylic acid-dependent pathway. Yang, Xiufen,Qiu, Dewen,Guo, Lihua,Zeng, Hongmei,Mao, Jianjun,Gao, Qiufeng.

[14]Transcriptomic And Proteomic Analysis Of L.ocusta Migratoria Eggs At D ifferent Embryonic Stages: Comparison For Diapause And Non-Diapause Regimes. Hao, K,Wang, J,Tu, XB,Whitman, DW,Zhang, ZH. 2017

[15]Comparative Transcriptomic Analysis Of Resistant A.nd Susceptible Alfalfa Cultivars ( Medicago Sativa L.) After Thrips Infestation. Tu, XB,Liu, ZK,Zhang, ZH. 2018

[16]A review of factors influencing diapause in bumble bee queens. Shi, Mengnan,Guo, Yueqin,Dong, Kun,Wu, Jie,Huang, Jiaxing. 2023

[17]Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Watermelon (Citrullus lanatus). Zhu, Yingchun,Yuan, Gaopeng,Gao, Bowen,An, Guolin,Li, Weihua,Si, Wenjing,Sun, Dexi,Liu, Junpu. 2022

[18]The protective effect of collagen peptides from bigeye tuna (Thunnus obesus) skin and bone to attenuate UVB-induced photoaging via MAPK and TGF-β signaling pathways. Yu Fu,Chongyang Li,Qiang Wang,Ruichang Gao,Xixi Cai,Shaoyun Wang,Yuhao Zhang. 2022

[19]NbTMP14 Is Involved in Tomato Spotted Wilt Virus Infection and Symptom Development by Interaction with the Viral NSm Protein. Zhan Jin,Shi Huiping,Li Weimin,Zhang Chao,Zhang Yongqiang. 2021

[20]Comparisons between Plant and Animal Stem Cells Regarding Regeneration Potential and Application. Lulu Liu,Lu Qiu,Yaqian Zhu,Lei Luo,Xinpei Han,Mingwu Man,Fuguang Li,Maozhi Ren,Yadi Xing. 2023

作者其他论文 更多>>