数字农科院2.0

Flexible Hydrophobic Paper-Based Microfluidic Field-Effect Biosensor Amplified by RNA-Cleaving DNAzyme-Based DNA Nanostructure for Mg2+ Detection

文献类型： 外文期刊

作者： Hui Wang;Yue He;Zhixue Yu;Ruipeng Chen;Zemeng Feng;Dongfei Chen;Waleid Mohamed El Sayed Shakweer;Fan Zhang;Xuemei Nan;Mukaddas Mijit;Benhai Xiong;Liang Yang;Xiangfang Tang

作者机构：

关键词： DNA nanoparticle;electrochemical electrode;hydrophobic paper;magnesium ion;microfluidic chip;similar DNA origami

期刊名称： Biosensors

ISSN： 2079-6374

年卷期： 2025 年 15 卷 7 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Magnesium ions (Mg2+) play an important role in animal health, with their concentration in the bloodstream serving as a key indicator for hypomagnesemia diagnosis. In this study, a flexible hydrophobic paper-based microfluidic field-effect biosensor was developed for point-of-care Mg2+ detection, which integrated flexible hydrophobic paper, semiconducting single-walled carbon nanotubes (SWNTs) and a Mg2+-specific RNA-cleaving DNAzyme(RCD)-based DNA nanostructure. Flexible hydrophobic paper was synthesized by using cellulose paper and octadecyltrichlorosilane, improving mechanical strength and decreasing biological interference. To achieve high sensitivity, the Mg2+-specific RCD was functionalized with SWNTs, and then repeatedly self-assembled two different Y-shaped DNAs to construct a DNA nanostructure based on a similar DNA origami technique. This proposed biosensor exhibited a linear detection range from 1 μM to 1000 μM, with a detection limit of 0.57 μM, demonstrating its great stability, selectivity, and anti-interference performance. This innovative design offers promising potential for Mg2+ monitoring in real applications.

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