数字农科院2.0

Development of a stable attenuated double-mutant of tobacco mosaic virus for cross-protection

文献类型： 外文期刊

作者： Xiaojie Xu;Shaoyan Jiang;Chunju Liu;Xujie Sun;Qing Zhu;Xiuzhai Chen;Pengchao Jiang;Fenglong Wang;Yanping Tian;Xiangdong Li

作者机构：

关键词： cross-protection;double-mutant;p126;tobacco mosaic virus;virulence

期刊名称： Journal of Integrative Agriculture

ISSN： 2095-3119

年卷期： 2024 年 23 卷 7 期

页码：

收录情况： SCIE（2024版） ; ; CSCD（2023-2024年度） ; ; 科技核心（2024版） ; ; 农林核心（2020版）

摘要： Tobacco (Nicotiana tabacum) and tomato (Solanum lycopersicum) are two major economic crops in China. Tobacco mosaic virus (TMV; genus Tobamovirus) is the most prevalent virus infecting both crops. Currently, some widely cultivated tobacco and tomato cultivars are susceptible to TMV and there is no effective strategy to control this virus. Cross-protection can be a safe and environmentally friendly strategy to prevent viral diseases. However, stable attenuated TMV mutants are scarce. In this study, we found that the substitutions in the replicase p126, arginine at position 196 (R196) with aspartic acid (D), glutamic acid at position 614 (E614) with glycine (G), serine at position 643 (S643) with phenylalanine (F), or D at position 730 (D730) with S, significantly reduced the virulence and replication of TMV. However, only the mutation of S643 to F reduced the RNA silencing suppression activity of TMV p126. A double-mutant TMV-E614G-S643F induced no visible symptom and was genetically stable through six successive passages in tobacco plants. Furthermore, our results showed that TMV-E614G-S643F double-mutant could provide effective protection against the wild-type TMV infection in tobacco and tomato plants. This study reports a promising mild mutant for cross-protection to control TMV in tobacco and tomato plants.

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