数字农科院2.0

Integrated transcriptomic and metabolomic analysis reveals drivers of protein and oil variation in cottonseed

文献类型： 外文期刊

作者： Chaoze Zhou;Yiwen Huang;Dayu Zhou;Yuzhen Wu;Shouyang Fu;Longyu Huang;Jun Peng;Meng Kuang

作者机构：

关键词： Cottonseed;GhNIR1;Metabolome;Oil content;Protein content;Transcriptome

期刊名称： Food Chemistry: Molecular Sciences

ISSN： 2666-5662

年卷期： 2025 年 11 卷

页码：

收录情况： ESCI（2025版）

摘要： The protein and oil content in cottonseed, known for their high quality, exhibits substantial variation across different cotton varieties. This study explored the regulatory mechanisms behind these differences by analyzing protein and oil accumulation patterns, transcriptomics, and metabolomics in two cotton varieties during seed development. Results showed that protein and oil rapidly accumulated between 15 and 30 days post-anthesis (DPA), but significant differences between varieties emerged after 40 DPA. Differentially expressed genes (DEGs) at 40 DPA were enriched in carbon allocation, fatty acid degradation, and nitrogen absorption pathways. Metabolomics identified lipids, lipid-like molecules, and organic acids as key differentially accumulated metabolites (DAMs). Furthermore, the gene GhNIR1, associated with nitrogen source absorption, was identified. Virus-induced gene silencing (VIGS) of this gene in cotton resulted in a significant reduction in protein content in the roots, stems, and leaves. These findings provide insights into protein and oil accumulation and offer genetic resources for improving cottonseed nutritional quality.

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