数字农科院2.0

Complete protection from Henosepilachna vigintioctopunctata by expressing long double-stranded RNAs in potato plastids

文献类型： 外文期刊

作者： Xu, Wenbo;Zhang, Miao;Li, Yangcun;He, Wanwan;Li, Shengchun;Zhang, Jiang

作者机构：

关键词： （0-1-2）double-stranded RNA;Henosepilachna vigintioctopunctata;pest control;plastid transformation;RNA interference

期刊名称： JOURNAL OF INTEGRATIVE PLANT BIOLOGY

ISSN： 1672-9072

年卷期： 2024 年

页码：

收录情况： SCIE（2024版） ; ; CSCD（2023-2024年度） ; ; 科技核心（2024版）

摘要： RNA interference (RNAi) has emerged as a powerful technology for pest management. Previously, we have shown that plastid-mediated RNAi (PM-RNAi) can be utilized to control the Colorado potato beetle, an insect pest in the Chrysomelidae family; however, whether this technology is suitable for controlling pests in the Coccinellidae remained unknown. The coccinellid 28-spotted potato ladybird (Henosepilachna vigintioctopunctata; HV) is a serious pest of solanaceous crops. In this study, we identified three efficient target genes (beta-Actin, SRP54, and SNAP) for RNAi using in vitro double-stranded RNAs (dsRNAs) fed to HV, and found that dsRNAs targeting beta-Actin messenger RNA (dsACT) induced more potent RNAi than those targeting the other two genes. We next generated transplastomic and nuclear transgenic potato (Solanum tuberosum) plants expressing HV dsACT. Long dsACT stably accumulated to up to 0.7% of the total cellular RNA in the transplastomic plants, at least three orders of magnitude higher than in the nuclear transgenic plants. Notably, the transplastomic plants also exhibited a significantly stronger resistance to HV, killing all larvae within 6 d. Our data demonstrate the potential of PM-RNAi as an efficient pest control measure for HV, extending the application range of this technology to Coccinellidae pests.

分类号：

  • 相关文献

[1]Complete protection from Henosepilachna vigintioctopunctata by expressing long double-stranded RNAs in potato plastids. Xu, Wenbo,Zhang, Miao,Li, Yangcun,He, Wanwan,Li, Shengchun,Zhang, Jiang. 2022

[2]Silencing of insect dsRNase genes enhances the plastid-mediated RNAi effect on the Colorado potato beetle. Li, Yangcun,Xu, Hong,He, Wanwan,Rong, Haoling,Li, Shengchun,Kim, Dae Sung,Han, Peng,Yang, Yang,Zhang, Jiang. 2023

[3]Foliar spray double-stranded RNA targeting HvIAP1 induces high larval and adult mortality in Henosepilachna vigintioctopunctata. Ji, Tianliang,Li, Langcheng,Zhu, Xin,Wang, Guirong. 2025

[4]Engineering a novel entomopathogenic strain Pseudomonas chlororaphis for efficient production of double-stranded RNAs and pest control. Xie, Mengmeng,Wang, Qinghai,Zhou, Niexin,Yu, Saisai,Zhang, Guiming,Yang, Sheng,Zhang, Jiang. 2025

[5]Virus-like particles empower RNAi for effective control of a Coleopteran pest. Jiang, Chunmei,Xiong, Dan,Li, Fujun,Huang, Xingwu,Li, Shengchun,Zhang, Jiang,Chang, Ling. 2025

[6]RNAi suppression of the nuclear receptor FTZ-F1 impaired ecdysis, pupation, and reproduction in the 28-spotted potato ladybeetle, Henosepilachna vigintioctopunctata. Zhuoqi Liu,Satyabrata Nanda,Chunxiao Yang,Shimin Chen,Mujuan Guo,Muhammad Musa Khan,Baoli Qiu,Youjun Zhang,Xuguo Zhou,Huipeng Pan. 2022

[7]RNAi effect in target and non-target pests correlates with the length of continuous matches in dsRNA sequences. Zhaoyang Li,Junna Liu,Satyabrata Nanda,Zexin Zhong,Xuming Luo,Xuguo Zhou,Youjun Zhang,Chunxiao Yang,Huipeng Pan. 2025

[8]The cleavage of dsRNA by StaufenC into siRNA affects the RNAi efficiency in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata. Zhaoyang Li,Ziqi Cheng,June Sun Yoon,Chunxiao Yang,Fei Li,Xuguo Zhou,Youjun Zhang,Huipeng Pan. 2025

[9]The effect of silencing arginine kinase by RNAi on the larval development of Helicoverpa armigera. Su, X. -F.,Lu, G. -Q.,Cheng, H. -M.,Liu, C. -X.,Liang, G. -M..

[10]Silencing of the BtTPS genes by transgenic plant-mediated RNAi to control Bemisia tabaci MED. Gong, Cheng,Yang, Zezhong,Hu, Yuan,Wu, Qingjun,Wang, Shaoli,Guo, Zhaojiang,Zhang, Youjun. 2021

[11]Delivery of Methoprene-Tolerant dsRNA to Improve RNAi Efficiency by Modified Liposomes for Pest Control. Su, Chenyu,Liu, Shanshan,Sun, Meixue,Yu, Qianlong,Li, Changyou,Graham, Robert I.,Wang, Xiufang,Wang, Xinwei,Xu, Pengjun,Ren, Guangwei. 2023

[12]HDAC3 Knockdown Dysregulates Juvenile Hormone and Apoptosis-Related Genes in Helicoverpa armigera. Huimin Chang,Zhenlu Xu,Wenkang Li,Chenggu Cai,Wenjing Wang,Pengliang Ge,Xue Jia,Yingge Li,Tianze Ding,Wei Ma,Ali Mohammad Banaei-Moghaddam,Huijuan Mo,Maozhi Ren. 2022

[13]Identification of leaf chloroplast-specific promoter to efficiently control of Colorado potato beetle with reduced dsRNA accumulation in potato tubers. Liao, Jianghua,Rong, Haoling,You, Lili,Xia, Kai,Wang, Minghui,Han, Peng,Li, Chao,Zhang, Jiang. 2023

[14]RNAi assays in the striped flea beetle (Phyllotreta striolata) suggest Psγ-COPI and PsArf1COPI as potential molecular targets for pest control. Mujuan Guo,Ran Gao,Satyabrata Nanda,Yingqiu Li,Changfei Guo,Xuguo Zhou,Youjun Zhang,Chunxiao Yang,Huipeng Pan. 2023

[15]Host-induced silencing of MpPar6 confers Myzus persicae resistance in transgenic rape plants. Qi Zhang,Wenqin Zhan,Chao Li,Ling Chang,Yi Dong,Jiang Zhang. 2024

[16]Silencing of the plant-derived horizontally transferred gene BtSC5DL effectively controls Bemisia tabaci MED. Liu, Yifan,Gong, Cheng,Hu, Yuan,Han, Haolin,Tian, Tian,Luo, Yili,Yang, Xin,Xie, Wen,Wu, Qingjun,Wang, Shaoli,Guo, Zhaojiang,Zhang, Youjun. 2025

[17]The plant-derived Bt11S gene in whitefly: a key player in reproduction and RNAi-based pest management. Gong, Cheng,Liu, Yifan,Hu, Yuan,Luo, Chen,Zhang, Youjun,Guo, Zhaojiang. 2025

[18]RNAi assay in the 28-spotted ladybeetle Henosepilachna vigintioctopunctata suggests nuclear receptor HR3 as a potential molecular target for pest control. Li, Zhaoyang,Ruan, Yalin,Zhou, Chuqiao,Liu, Junna,Wang, Yajie,Yoon, June-Sun,Zhou, Xuguo,Zhang, Youjun,Yang, Chunxiao,Pan, Huipeng. 2025

[19]Identification and screening of potential RNAi targets in the bean bug Riptortus pedestris via virus-induced gene silencing and root soaking. Chen, Zi-Xu,Xuan, Jing-Li,Willadsen, Peter C.,Lorenzen, Marce,Li, Jin-Bu,Liu, Wan-Xue,Zhang, Ya-Nan,Li, Shu-Peng. 2025

[20]Transformer is involved in female sex determination in Bemisia tabaci. Liu, Yating,Wang, Wenlu,Wang, Yina,Zhou, Xuguo,Xie, Wen,Zhang, Youjun. 2025

作者其他论文 更多>>