数字农科院2.0

Virus-like particles empower RNAi for effective control of a Coleopteran pest

文献类型： 外文期刊

作者： Jiang, Chunmei;Xiong, Dan;Li, Fujun;Huang, Xingwu;Li, Shengchun;Zhang, Jiang;Chang, Ling

作者机构：

关键词： （0-2-2）RNA interference;hairpin RNA (hpRNA);virus-like particle;Henosepilachna vigintioctopunctata;pest control;Solanum;biopesticide;spray-induced gene silencing (SIGS)

期刊名称： ENTOMOLOGIA GENERALIS

ISSN： 0171-8177

年卷期： 2025 年 45 卷 4 期

页码：

收录情况： SCIE（2025版）

摘要： RNA interference (RNAi) technology is emerging as a promising and effective alternative for pest control. However, its application has faced challenges due to the inconsistent efficacy of RNAi across different pest species. Factors contributing to this variability include the instability and insufficient internalization of double-stranded RNA (dsRNA). To address these challenges and enhance RNAi efficiency, we have employed bacteriophage MS2 virus-like particles (VLPs) as a delivery platform. MS2 VLPs can autonomously assemble into a capsid and encapsulate RNA molecules with pac site. This approach is utilized to package hpRNA within Escherichia coli for the management of the destructive pest Henosepilachna vigintioctopunctata, a coleopteran insect. We tested three E. coli strains-BL21 (DE3), HT115 (DE3) and BL21 (DE3)-RNase III-for the expression of coat protein (CP) and hpACT. Among these, BL21 (DE3)-RNase III-yielded higher levels of CP and hpACT. Transmission electron microscopy analysis revealed that the CP expressed in BL21 (DE3)-RNase III-successfully encapsulated hpACT within MS2 VLPs. The hpACT encapsulated by MS2 showed resistance to RNase III and the intestinal fluid of H. vigintioctopunctata. By applying BL21 (DE3)-RNase III-cells expressing CP and hpACT through painting or spraying onto the detached leaves of Solanum nigrum and entire plants of S. tuberosum, RNAi efficiency against H. vigintioctopunctata was enhanced. Our study suggests that the potential of the MS2 VLP-coated hpRNA strategy in advancing innovative RNAi-based pest control applications.

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