数字农科院2.0

The genome of a wild Medicago species provides insights into the tolerant mechanisms of legume forage to environmental stress

文献类型： 外文期刊

作者： Wang Tianzuo;Ren Lifei;Li Caihong;Zhang Di;Zhang Xiuxiu;Zhou Gang;Gao Dan;Chen Rujin;Chen Yuhui;Wang Zhaolan;Shi Fengling;Farmer Andrew D.;Li Yansu;Zhou Mengyan;Young Nevin D.;Zhang Wen-Hao

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关键词： Comparative genomics; Domestication; Drought tolerance; Evolution; Genome; Medicago ruthenica; Single-nucleotide polymorphism; Transcriptome; Wild genetic resource

期刊名称： BMC Biology

ISSN： 1741-7007

年卷期： 2021 年 19 卷 1 期

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收录情况： JCR（2021版）

摘要： Background: Medicago ruthenica, a wild and perennial legume forage widely distributed in semi-arid grasslands, is distinguished by its outstanding tolerance to environmental stress. It is a close relative of commonly cultivated forage of alfalfa (Medicago sativa). The high tolerance of M. ruthenica to environmental stress makes this species a valuable genetic resource for understanding and improving traits associated with tolerance to harsh environments. Results: We sequenced and assembled genome of M. ruthenica using an integrated approach, including PacBio, Illumina, 10×Genomics, and Hi-C. The assembled genome was 904.13 Mb with scaffold N50 of 99.39 Mb, and 50,162 protein-coding genes were annotated. Comparative genomics and transcriptomic analyses were used to elucidate mechanisms underlying its tolerance to environmental stress. The expanded FHY3/FAR1 family was identified to be involved in tolerance of M. ruthenica to drought stress. Many genes involved in tolerance to abiotic stress were retained in M. ruthenica compared to other cultivated Medicago species. Hundreds of candidate genes associated with drought tolerance were identified by analyzing variations in single nucleotide polymorphism using accessions of M. ruthenica with varying tolerance to drought. Transcriptomic data demonstrated the involvements of genes related to transcriptional regulation, stress response, and metabolic regulation in tolerance of M. ruthenica. Conclusions: We present a high-quality genome assembly and identification of drought-related genes in the wild species of M. ruthenica, providing a valuable resource for genomic studies on perennial legume forages. © 2021, The Author(s).

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