数字农科院2.0

siRNA self-assembled carboxymethyl chitosan and pymetrozine nucleic pesticides for enhanced control of Sogatella furcifera: Bidirectional transduction and detoxification inhibition

文献类型： 外文期刊

作者： Changwei Gong;Wei Wang;Yanxin Ma;Xiaoxu Zhan;Anchun Peng;Jian Pu;Jizhi Yang;Xiangyang Yuan;Xuegui Wang

作者机构：

关键词： Carboxymethyl chitosan;Insecticide resistance management;Insecticides delivery;RNAi pesticides;Sogatella furcifera

期刊名称： Carbohydrate Polymers

ISSN： 0144-8617

年卷期： 2025 年 354 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Sogatella furcifera (Horváth) is a significant migratory pest in southeast Asia and northern Australia, exhibiting a notable resistance to the conventional insecticides, and RNAi pesticide is a promising approach for managing resistance. However, the poor stability and difficulty in delivery hinders its wide application. Here, we present a self-assembled siRNAs, carboxymethyl chitosan (CMCS) and pymetrozine complex (abbreviated as PM-CMCS/siRNAs micro-complex) to facilitate the control of S. furcifera in rice. When the transcription factors USP and Kr[sbnd]H1 were interfered with dsUSP or dsKr-H1, a mortality of 84.4 % ~ 88.9 % was observed at a concentration of 150 μg/mL of pymetrozine, indicating a potential strategy for managing pymetrozine resistance. With the envelope of CMCS, the micro-complex effectively enhances the siRNA stability >96 h, downregulates the expression of USP, Kr[sbnd]H1, and CYP6FJ3, leading to a mortality of 100 % at 100 μg/mL pymetrozine. Moreover, the time-dependent disintegration of micro-complex induces the release of lesser PM-CMCS/siRNAs, allowing that more fluorescently labeled complexes were enriched in the phloem of rice via the apoplastic pathway. The integration of PM-CMCS/siRNAs enable precise enrichment in phloem and effective management of pest resistance by silencing their detoxification genes, and promises an environmental-friendly strategy for controlling devastating pest.

分类号：

  • 相关文献

[1]Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction. Changwei Gong,Wei Wang,Yanxin Ma,Xiaoxu Zhan,Anchun Peng,Jian Pu,Jizhi Yang,Xuegui Wang. 2024

[2]Changes of Sex Pheromone Communication Systems Associated with Tebufenozide and Abamectin Resistance in Diamondback Moth, Plutella xylostella (L.). Dong, Shuang-Lin,Cao, Guang-Chun.

[3]Western flower thrips resistance to insecticides: detection, mechanisms and management strategies. Gao, Yulin,Lei, Zhongren,Reitz, Stuart R..

[4]Editorial: The side effects of insecticides on insects and the adaptation mechanisms of insects to insecticides. Youhui Gong,Ting Li,Adil Hussain,Xiaoming Xia,Qiangli Shang,Asad Ali. 2023

[5]Antimicrobial activity and cytotoxicity of N-2-HACC and characterization of nanoparticles with N-2-HACC and CMC as a vaccine carrier. Li, Wei,Chen, Gang,Guo, Chen,Zhang, Yang,Kang, Hong,Zhao, Kai,Jin, Zheng,Zhang, Xu,Wu, Hao,Cao, Hongwei,Li, Wei,Zhang, Yang,Wang, Yunfeng,Zhao, Kai.

[6]Recyclable magnetic carboxymethyl chitosan/calcium alginate - cellulase bioconjugates for corn stalk hydrolysis. Jiang, Jianfang,Zhao, Jiaqi,He, Chunyang,Cui, Baodong,Xiong, Jun,Ao, Juan,Xiang, Guangyan,Jiang, Hao.

[7]Recyclable Magnetic Carboxymethyl Chitosan/Calcium Alginate -. Cellulase Bioconjugates For C orn Stalk Hydrolysis. Jiang, JF, Zhao, JQ, He, CY, Cui, BD, Xiong, J, Jiang, H, Ao, J, Xiang, GY. 2017

[8]A berberine hydrochloride-carboxymethyl chitosan hydrogel protects against Staphylococcus aureus infection in a rat mastitis model. Jinjin Tong,Xiaolin Hou,Defeng Cui,Wu Chen,Hua Yao,Benhai Xiong,Lirong Cai,Hua Zhang,Linshu Jiang. 2022

[9]Characterisation, slow-release, and antibacterial properties of carboxymethyl chitosan/inulin hydrogel film loaded with novel antilisterial durancin GL. Debao Wang,Lihui Du,Zhilan Sun,Fang Liu,Dequan Zhang,Daoying Wang. 2023

[10]Carboxymethyl chitosan/polyvinyl alcohol hydrogel films by incorporating MSNs as ε-PL carrier with pH-responsive controlled release and antibacterial properties. Debao Wang,Simin Fan,Xin Li,Li Chen,Xiangyuan Wen,Yuqian Xu,Chaoqiao Zhu,Chengli Hou,Dequan Zhang. 2024

[11]Chitosan-based metal–organic frameworks for improving the oral delivery and antibacterial activity of natural flavonoids. Shuzhang Liu,Hongling Lin,Haisheng Xie,Yuexin Chen,Xiaofei Shang,Yanhong Yong,Shengyi Wang,Yongxin She,Xianghong Ju,Jiyu Zhang,Xingbin Ma,A. M. Abd El-Aty. 2025

[12]Diversity, Tissue Localization, And Infection Pattern Of Bacterial Symbionts Of The White-Backed Planthopper, Sogatella Furcifera (Hemiptera: Delphacidae). Hua, Hongxia,Li, Pei,Li, Fei,Li, Fei,Hou, Maolin,Hou, Maolin,Hou, Maolin,Wang, Fulian. 2020

[13]Characterization Of A Bacterial Symbiont A.saia Sp. In The W hite-Backed Planthopper, Sogatella Furcifera, And Its Effects On Host Fitness. Ali, Asad,Li, Fei,Hou, Maolin,Ali, Asad,Hou, Maolin,Hou, Maolin,Li, Fei,Hua, Hongxia. 2019

[14]Thirteen microsatellite loci for Laodelphax striatellus and cross amplification in related taxa. Liu, Yudi,Hou, Maolin,Zhang, Baoqin. 2014

[15]Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions. Han Nai-shun,Tian Jun-ce,Hu Cui,Shen Zhi-cheng,Ye Gong-yin,Tian Jun-ce,Peng Yu-fa,Guo Yu-yuan. 2014

[16]Reference Gene Selection and Evaluation for Gene Expression Studies Using qRT-PCR in the White-Backed Planthopper, Sogatella furcifera (Hemiptera: Delphacidae). Hou, Mao-lin,Liu, Yu-di.

[17]Presence of a short repeat sequence in internal transcribed spacer (ITS) 1 of the rRNA gene of Sogatella furcifera (Hemiptera: Delphacidae) from geographically different populations in Asia. Matsumoto, Yukiko,Sato, Yuki,Noda, Hiroaki,Fu, Qiang,Matsumura, Masaya.

[18]Fipronil resistance in the whitebacked planthopper (Sogatella furcifera): possible resistance mechanisms and cross-resistance. Li, Jian,Shao, Ying,Liu, Zewen,Tang, Jian,Yang, Baojun.

[19]A comparative study on population development patterns of Sogatella furcifera between tropical and subtropical areas. Hu, Yang,Cheng, Jiaan,Zhu, Zengrong,Heong, Kong Luen,Fu, Qiang,He, Jiachun,Hu, Yang,Cheng, Jiaan,Zhu, Zengrong,Heong, Kong Luen,Fu, Qiang,He, Jiachun,Hu, Yang,Cheng, Jiaan,Zhu, Zengrong,Heong, Kong Luen,Heong, Kong Luen.

[20](E)-beta-caryophyllene functions as a host location signal for the rice white-backed planthopper Sogatella furcifera. Wang, Qi,Xin, Zhaojun,Li, Jiancai,Hu, Lingfei,Lou, Yonggen,Lu, Jing,Wang, Qi,Xin, Zhaojun.

作者其他论文 更多>>