数字农科院2.0

Sensitive and Specific Exonuclease III-Assisted Recombinase-Aided Amplification Colorimetric Assay for Rapid Detection of Nucleic Acids

文献类型： 外文期刊

作者： Zhou, Yuan;Zhang, Jinfu;Sun, Haowen;Tao, Dagang;Xu, Bingrong;Han, Xiaosong;Ren, Ruimin;Ruan, Jinxue;Steinaa, Lucilla;Hemmink, Johanneke D.;Han, Jianlin;Li, Xinyun;Xu, Jing;Zhao, Shuhong;Xie, Shengsong;Zhao, Changzhi

作者机构：

关键词： nucleic acid detection;exonuclease III;recombinase-aidedamplification;African swine fever virus

期刊名称： ACS SYNTHETIC BIOLOGY

ISSN： 2161-5063

年卷期： 2023 年 12 卷 10 期

页码：

收录情况： SCIE（2023版）

摘要： The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplification (RAA) with the exonuclease III (Exo III)-assisted signal amplification into a platform for sensitive and specific detection of nucleic acids of African swine fever virus (ASFV). We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/mu L in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. In addition, to avoid aerosol contamination, a one-tube RAA-Exo III colorimetric assay was also established for the accurate detection of ASFV in clinical samples. Taken together, we developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric-assay-based nucleic acid detection platform.

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