数字农科院2.0

Fine mapping and candidate gene analysis of qFL-A12-5: a fiber length-related QTL introgressed from Gossypium barbadense into Gossypium hirsutum

文献类型： 外文期刊

作者： Xianghui Xiao;Ruixian Liu;Juwu Gong;Pengtao Li;Ziyin Li;Wankui Gong;Aiying Liu;Qun Ge;Xiaoying Deng;Shaoqi Li;Quanjia Chen;Hua Zhang;Renhai Peng;Yan Peng;Haihong Shang;Jingtao Pan;Yuzhen Shi;Quanwei Lu;Youlu Yuan

作者机构：

期刊名称： Theoretical and Applied Genetics

ISSN： 0040-5752

年卷期： 2023 年 136 卷 3 期

页码：

收录情况： SCIE（2023版） ; ; EI（2023版）

摘要： Key message: The fiber length-related qFL-A12-5 identified in CSSLs introgressed from Gossypium barbadense into Gossypium hirsutum was fine-mapped to an 18.8 kb region on chromosome A12, leading to the identification of the GhTPR gene as a potential regulator of cotton fiber length. Abstract: Fiber length is a key determinant of fiber quality in cotton, and it is a key target of artificial selection for breeding and domestication. Although many fiber length-related quantitative trait loci have been identified, there are few reports on their fine mapping or candidate gene validation, thus hampering efforts to understand the mechanistic basis of cotton fiber development. Our previous study identified the qFL-A12-5 associated with superior fiber quality on chromosome A12 in the chromosome segment substitution line (CSSL) MBI7747 (BC4F3:5). A single segment substitution line (CSSL-106) screened from BC6F2 was backcrossed to construct a larger segregation population with its recurrent parent CCRI45, thus enabling the fine mapping of 2852 BC7F2 individuals using denser simple sequence repeat markers to narrow the qFL-A12-5 to an 18.8 kb region of the genome, in which six annotated genes were identified in Gossypium hirsutum. Quantitative real-time PCR and comparative analyses led to the identification of GH_A12G2192 (GhTPR) encoding a tetratricopeptide repeat-like superfamily protein as a promising candidate gene for qFL-A12-5. A comparative analysis of the protein-coding regions of GhTPR among Hai1, MBI7747, and CCRI45 revealed two non-synonymous mutations. The overexpression of GhTPR resulted in longer roots in Arabidopsis, suggesting that GhTPR may regulate cotton fiber development. These results provide a foundation for future efforts to improve cotton fiber length.

分类号：

  • 相关文献
作者其他论文 更多>>