数字农科院2.0

A 48-bp deletion within the promoter of the BnaC9.APT5 gene results in elevated seed number per silique in Brassica napus

文献类型： 外文期刊

作者： Cui, Yixin;Lv, Mengjie;Xin, Shuangshuang;Dong, Hongli;Zhou, Xiangchun;Lan, Xueyi;Zhang, Danhui;Tang, Yongqing;Liu, Zhi;Cai, Guangqin;He, Yajun;Jung, Christian;Qian, Wei;Li, Xiaorong

作者机构：

关键词： adenine phosphoribosyltransferase 5;cytokinin;natural variation;rapeseed;seed number per silique

期刊名称： NEW PHYTOLOGIST

ISSN： 0028-646X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Seed number per silique (SNPS) is an important determinant for seed yield in rapeseed (Brassica napus). Here, the molecular mechanism of BnaC9.APT5 (Adenine phosphoribosyltransferase 5) regulating SNPS was identified by combining gene function with biochemical analyses. Overexpression (OE) of Arabidopsis Adenine Phosphoribosyltransferase 5 (AtAPT5) and ectopic expression of BnaC9.APT5 in Arabidopsis thaliana increased SNPS by c. 9%. By contrast, the Arabidopsis knockout mutant had 18% fewer seeds per silique. OE of BnaC9.APT5 in rapeseed increased SNPS by c. 11% through upregulating cytokinin (CK) biosynthesis genes. The enzyme activity analysis showed that BnaC9.APT5 converts adenine to adenosine monophosphate. Spraying the Arabidopsis knockout mutant with 6-benzylaminopurine increased SNPS by 34%. The treatment of the Arabidopsis AtAPT5 and rapeseed BnaC9.APT5 OE lines with the CK biosynthesis inhibitor lovastatin reduced SNPS by c. 7% and 9%, respectively. Thus, BnaC9.APT5 controls SNPS via the CK metabolism in rapeseed. A 48-bp InDel in the BnaC9.APT5 promoter was significantly associated with gene expression and SNPS and has been selected during rapeseed 'double-low' quality breeding. Our data provide clear evidence of the role of the BnaC9.APT5 gene for the genetic improvement of modern rapeseed.

分类号：

  • 相关文献

[1]城郊农区油菜立体套种模式研究(英文). 杨良金,夏晓进,张春雷,陶文才,杨鑫海,成海燕,尹金凤. 2007

[2]油菜栽培管理模拟优化决策系统试验示范(英文). 张春雷,曹洪鑫,李光明,李锋,赵懿. 2007

[3]中国的油菜生产与栽培科学技术研究(英文). 王汉中,官春云,张春雷. 2007

[4]Identification of natural allelic variation in TTL1 controlling thermotolerance and grain size by a rice super pan-genome. Lin, Yarong,Zhu, Yiwang,Cui, Yuchao,Qian, Hongge,Yuan, Qiaoling,Chen, Rui,Lin, Yan,Chen, Jianmin,Zhou, Xishi,Shi, Chuanlin,He, Huiying,Hu, Taijiao,Gu, Chenbo,Yu, Xiaoman,Zhu, Xiying,Wang, Yuexing,Qian, Qian,Zhang, Cuijun,Wang, Feng,Shang, Lianguang. 2023

[5]Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Gu, Riliang,Zhao, Li,Zhang, Ying,Chen, Xiaoping,Bao, Juan,Zhao, Jinfeng,Wang, Zhangying,Fu, Junjie,Liu, Tingsong,Wang, Jianhua,Wang, Guoying. 2006

[6]A novel DREB transcription factor from Halimodendron halodendron leads to enhance drought and salt tolerance in Arabidopsis. Ma, J. -T.,Wang, Z. -L.,Ma, J. -T.,Yin, C. -C.,Guo, Q. -Q.,Zhou, M. -L.,Wu, Y. -M.,Guo, Q. -Q..

[7]Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process. Qiu, Lingling,Jiang, Bo,Fang, Jia,Shen, Yike,Fang, Zhongxiang,Kumar, Saravana R. M.,Yan, Daoliang,Zheng, Bingsong,Fang, Jia,Shen, Yike,Fang, Zhongxiang,Kumar, Saravana R. M.,Yan, Daoliang,Zheng, Bingsong,Yi, Keke,Shen, Chenjia. 2016

[8]Transcriptome Profiling Reveals Auxin and Cytokinin Regulating Somatic Embryogenesis in Different Sister Lines of Cotton Cultivar CCRI24. Xu Zhenzhen. 2013

[9]Increased photosynthetic capacity in response to nitrate is correlated with enhanced cytokinin levels in rice cultivar with high responsiveness to nitrogen nutrients. Song, Wenjing,Li, Jiao,Sun, Huwei,Huang, Shuangjie,Gong, Xianpo,Ma, Qunyu,Zhang, Yali,Xu, Guohua,Song, Wenjing.

[10]Levels of cytokinins in the ovules of cotton mutants with altered fiber development. Chen, JG,Du, XM,Zhou, X,Zhao, HY. 1997

[11]A cryptic inhibitor of cytokinin phosphorelay controls rice grain size. Dapu Liu,He Zhao,Yunhua Xiao,Guoxia Zhang,Shouyun Cao,Wenchao Yin,Yangwen Qian,Yanhai Yin,Jinsong Zhang,Shouyi Chen,Chengcai Chu,Hongning Tong. 2022

[12]Effects Of Exogenous 6-Benzyladenine On D.warfing, Shoot Branching, And Y ield Of Tea Plant (Camellia Sinensis). Zhang, LP, Shen, C, Wei, JP, Han, WY. 2018

[13]Cyp71D8L Is A Key Regulator Involved In Growth And Stress Responses By Mediating Gibberellin Homeostasis In Rice. Zhou, JH, Li, ZY, Xiao, GQ, Zhai, MJ, Pan, XW, Huang, RF, Zhang, HW. 2020

[14]VaAPRT3 Gene is Associated With Sex Determination in Vitis amurensis. Yan Men,Ji Rui Li,Hai Lin Shen,Yi Ming Yang,Shu Tian Fan,Kun Li,Yin Shan Guo,Hong Lin,Zhen Dong Liu,Xiu Wu Guo. 2021

[15]MdIPT8, an isopentenyl transferase enzyme, enhances the resistance of apple to Colletotrichum gloeosporioides infection. Jiajun Shi,Feng Zhang,Yufei Su,Qiu Jiang,Yuhong Yuan,Xiaolin Nie,Ying Zhou,Xinxu Zhang,Zhigang Wang,Feng Wang,Yue Ma. 2022

[16]Leaf cytokinin accumulation promotes potato growth in mixed nitrogen supply by coordination of nitrogen and carbon metabolism. Wei Zhang,Xu Wu,Daojian Wang,Daxia Wu,Yihan Fu,Chunsong Bian,Liping Jin,Yali Zhang. 2022

[17]Antioxidant system was triggered to alleviate salinity stress by cytokinin oxidase/dehydrogenase gene GhCKX6b-Dt in cotton. Liu, Mengyue,Cui, Yupeng,Peng, Fanjia,Wang, Shuai,Cui, Ruifeng,Liu, Xiaoyu,Zhang, Yuexin,Huang, Hui,Fan, Yapeng,Jiang, Tiantian,Feng, Xixian,Lei, Yuqian,Ni, Kesong,Han, Mingge,Chen, Wenhua,Meng, Yuan,Wang, Junjuan,Chen, Xiugui,Lu, Xuke,Wang, Delong,Guo, Lixue,Zhao, Lanjie,Jiang, Jing,Ye, Wuwei. 2023

[18]Enhanced Post-Drought Compensatory Growth and Water Utilization in Maize via Rhizosphere Soil Nitrification by Heterotrophic Ammonia-Oxidizing Bacteria. Xiao Ling Wang,Shi Shi Tian,Hao Yu,Run Hong Sun,Lin Qi,Peng Song,Shen Jiao Yang. 2023

[19]Metabolic, transcriptional, and hormonal responses of Panax ginseng C. A. Meyer to nitrogen deficiency. Hao Liang,Hai Sun,Cai Shao,Bochen Lv,Jiapeng Zhu,Weiyu Cao,Jixin Zhou,Yayu Zhang. 2025

[20]Improving dryland maize productivity and water efficiency with heterotrophic ammonia-oxidizing bacteria via nitrification and cytokinin activity. Wang, Xiaoling,Cao, Jiawei,Sun, Runhong,Liu, Wei,Qi, Lin,Song, Peng,Yang, Shenjiao. 2024

作者其他论文 更多>>