数字农科院2.0

Dihydroquercetin attenuated Prevotella copri-caused intestinal injury by modulating gut microbiota and bile acids in weaned piglets

文献类型： 外文期刊

作者： Long Wang;Ruizhi Hu;Siqi Ma;Xizi Yang;Jiatai Gong;Hongkun Xiang;Mingkun Shi;Xupeng Yuan;Liang Chen;Hongfu Zhang;Bie Tan;Xi He;Jianhua He;Shusong Wu

作者机构：

关键词： Bile acid;Dihydroquercetin;Gut microbiota;Piglet;Prevotella copri

期刊名称： Animal Nutrition

ISSN： 2405-6545

年卷期： 2025 年 20 卷

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： Gut microbiota disruption during the weaning process is a significant factor of intestinal injury. Our previous studies have suggested that Prevotella may play a critical role in causing intestinal inflammation. This study aimed to clarify the impact of Prevotella copri on intestinal injury and the protecting effect by dihydroquercetin (DHQ) in weaned piglets. A total of 108 healthy Duroc × Landrace × Yorkshire weaned piglets, aged 21 d, were randomly allocated into 3 groups with 6 replicates and 6 piglets per replicate. The piglets were the following diets for 28 d: 1) a basal diet, 2) basal diet containing 1.0 × 108 CFU/kg P. copri, 3) basal diet supplemented with 1.0 × 108 CFU/kg P. copri and 100 mg/kg DHQ. Results showed that P. copri decreased significantly the average daily gain (ADG) (P < 0.001), which was recovered by supplementation of DHQ with decreased serum levels of malondialdehyde (MDA), interleukin (IL)-2 and IL-8 but increased total superoxide dismutase (T-SOD) activity and IL-10 in weaned piglets (P < 0.001). Moreover, DHQ increased the expression of tight junction proteins (claudin-2, occludin and tight junction protein zonula occludens protein-1 (ZO-1) and the mRNA expression of glutathione peroxidase 4 (GPX-4) in ileum (P < 0.001). Intestinal flora analysis showed that P. copri increased the relative abundance of Prevotella (P = 0.026) and Eubacterium coprostanoligenes group (P < 0.001), but decreased the relative abundance of Lachnospiraceae NK4A136 group (P < 0.001), while supplementation of DHQ reduced the relative abundance of Prevotella (P = 0.026). Metabolomics results indicated that P. copri enhanced the content of 12-OH bile acid, but decreased the contents of glycodeoxycholic acid (GDCA) and glycochenodeoxycholic acid (GCDCA) (P < 0.001), while DHQ reduced the 12-OH bile acid content (P < 0.001) and increased the GDCA content (P = 0.020). In summary, P. copri caused intestinal injury and reduced growth performance in weaned piglets, and DHQ showed a protective effect by modulating gut microbiota and bile acids metabolism.

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