数字农科院2.0

Analysis of PAT1 subfamily members in the GRAS family of upland cotton and functional characterization of GhSCL13-2A in Verticillium dahliae resistance

文献类型: 外文期刊

作者: Chen, Chen;Lu, Li-Li;Ma, Shu-Ya;Zhao, Yan-Peng;Wu, Na;Li, Wen-Jie;Ma, Li;Kong, Xian-Hui;Xie, Zong-Ming;Hou, Yu-Xia

作者机构:

关键词: GRAS;PAT1;GhSCL13-2A;Verticillium dahliae;Upland cotton

期刊名称: PLANT CELL REPORTS

ISSN: 0721-7714

年卷期: 2023 年

页码:

收录情况: SCIE(2023版)

摘要: Key messageGhSCL13-2A, a member of the PAT1 subfamily in the GRAS family, positively regulates cotton resistance to Verticillium dahliae by mediating the jasmonic acid and salicylic acid signaling pathways and accumulation of reactive oxygen species.Verticillium wilt (VW) is a devastating disease of upland cotton (Gossypium hirsutum) that is primarily caused by the soil-borne fungus Verticillium dahliae. Scarecrow-like (SCL) proteins are known to be involved in plant abiotic and biotic stress responses, but their roles in cotton defense responses are still unclear. In this study, a total of 25 GhPAT1 subfamily members in the GRAS family were identified in upland cotton. Gene organization and protein domain analysis showed that GhPAT1 members were highly conserved. GhPAT1 genes were widely expressed in various tissues and at multiple developmental stages, and they were responsive to jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) signals. Furthermore, GhSCL13-2A was induced by V. dahliae infection. V. dahliae resistance was enhanced in Arabidopsis thaliana by ectopic overexpression of GhSCL13-2A, whereas cotton GhSCL13-2A knockdowns showed increased susceptibility. Levels of reactive oxygen species (ROS) and JA were also increased and SA content was decreased in GhSCL13-2A knockdowns. At the gene expression level, PR genes and SA signaling marker genes were down-regulated and JA signaling marker genes were upregulated in GhSCL13-2A knockdowns. GhSCL13-2A was shown to be localized to the cell membrane and the nucleus. Yeast two-hybrid and luciferase complementation assays indicated that GhSCL13-2A interacted with GhERF5. In Arabidopsis, V. dahliae resistance was enhanced by GhERF5 overexpression; in cotton, resistance was reduced in GhERF5 knockdowns. This study revealed a positive role of GhSCL13-2A in V. dahliae resistance, establishing it as a strong candidate gene for future breeding of V. dahliae-resistant cotton cultivars.

分类号:

  • 相关文献

[1]Identification and expression analysis of arabinogalactan protein genes in cotton reveal the function of GhAGP15 in Verticillium dahliae resistance. Wen Jie Li,Na Wu,Chen Chen,Yan Peng Zhao,Yu Xia Hou. 2022

[2]Analysis of the PRA1 Genes in Cotton Identifies the Role of GhPRA1.B1-1A in Verticillium dahliae Resistance. Na Wu,Wen Jie Li,Chen Chen,Yan Peng Zhao,Yu Xia Hou. 2022

[3]Flagellin C decreases the expression of the Gossypium hirsutum cation/proton exchanger 3 gene to promote calcium ion, hydrogen peroxide, and nitric oxide and synergistically regulate the resistance of cotton to Verticillium wilt. Zhou, Heng,Wang, Yi,Zhang, Yihao,Xie, Yijing,Nadeem, Hasan,Tang, Canming. 2022

[4]GhERF.B4-15D: A Member of ERF Subfamily B4 Group Positively Regulates the Resistance against Verticillium dahliae in Upland Cotton. Panpan Wang,Yanpeng Zhao,Na Wu,Muhammad Tehseen Azhar,Yuxia Hou,Haihong Shang. 2023

[5]Promotion of apoplastic oxidative burst by artificially selected GhCBSX3A enhances Verticillium dahliae resistance in upland cotton. Zhang, Yihao,Yuan, Yuan,Xi, Hongfang,Zhang, Yaning,Gao, Chenxu,Ma, Meng,Huang, Qian,Li, Fuguang,Yang, Zhaoen. 2024

[6]Integrative GWAS and transcriptomics reveal GhAMT2 as a key regulator of cotton resistance to Verticillium wilt. Long Wang,Yonglin Yang,Jianghong Qin,Qifeng Ma,Kaikai Qiao,Shuli Fan,Yanying Qu. 2025

[7]Identification of Candidate Genes Controlling Resistance to Verticillium dahliae Using CCRI70 Recombinant Inbred Line Population in Gossypium hirsutum. Zhao, Yanpeng,Li, Tongyi,Hu, Fuxin,Wang, Panpan,Azhar, Muhammad Tehseen,Yan, Haoliang,Gong, Juwu,Yuan, Youlu,Shang, Haihong. 2025

[8]普通烟草GRAS基因家族鉴定及表达模式分析. 孙晋浩,吴昌健,管恩森,熊党安,周天宇,陈悦,刘晓姗,赵清海,王大海,李志远,马志远,田震. 2024

[9]Comparative Analysis of GRAS Genes in Six Cucurbitaceae Species Provides Insights into Their Evolution and Function. Zhang, Qiqi,Wang, Cui,He, Jun,Xu, Yuanchao,Li, Hongbo,Sun, Tianshu,Chai, Sen,Xin, Hongjia,Xu, Kuipeng,Mou, Shan,Zhang, Zhonghua. 2023

[10]Identification Of Tpx2 Gene Family I.n Upland Cotton And I ts Functional Analysis In Cotton Fiber Development. Zhang, Shuya,Zhang, Lipeng,Shi, Yuzhen,Jiang, Xiao,Ge, Qun,Gong, Wankui,Jia, Tingting,Li, Junwen,Shang, Haihong,Fan, Liqiang,Lei, Kang,Zhang, Zhibin,Zhen, Zhang,Deng, Xiaoying,Peng, Huo,Fan, Senmiao,Huang, Jinyong,Liu, Aiying,Shang, Haihong,Yuan, Youlu,Yuan, Youlu,Gong, Juwu,Zou, Xianyan. 2019

[11]Quantitative Trait Locus Mapping For P.lant Height And Branch N umber In An Upland Cotton Recombinant Inbred Line With An Snp-Based High-Density Genetic Map. Shi, Yuzhen,Xu, Aixia,Shang, Haihong,Liu, Aiying,Shang, Haihong,Yuan, Youlu,Zhang, Zhibin,Zou, Xianyan,Zhang, Zhen,Yuan, Youlu,Zhang, Zhen,Gong, Wankui,Gong, Juwu,Jiang, Xiao,Huang, Zhen,Fan, Senmiao,Ge, Qun,Deng, Xiaoying,Fan, Liqiang,Li, Junwen,Lei, Kang. 2019

[12]Genome Resequencing Reveals Genetic Variation B.etween The Parents Of A n Elite Hybrid Upland Cotton. Ma, Xiongfeng,He, Kunlun,Li, Wei,Pei, Xiaoyu,Ren, Zhongying,Song, Chengxiang,Liu, Yangai,Yang, Daigang,Sun, Kuan,Wang, Zhenyu,Song, Chengxiang,Zhang, Fei,Zhou, Xiaojian. 2018

[13]Association Mapping Analysis Of Fiber Y.ield And Quality Traits I n Upland Cotton (Gossypium Hirsutum L.). Ademe, Mulugeta Seyoum,Du, Xiongming,Li, Lin,Ma, Zhiying,Sun, Junling,Cai, Zhongmin,Wang, Liru,Zhu, Haiyong,Jia, Yinhua,He, Shoupu,Wang, Qinglian,Yang, Jinlong,Zhang, Jinbiao,Pang, Baoyin,Zhang, Xin,Liu, Jinhai,Qin, Hongde,Zhang, Xuelin,Zhou, Guanyin,Li, Zhikun,Huang, Aifen,Pan, Zhaoe,Yang, Jun,Liu, Hui,Yi, Xianda. 2017

[14]Genome-Wide Comparative Transcriptome Analysis Of C.ms-D2 And Its Maintainer A nd Restorer Lines In Upland Cotton. Zhang, Bingbing,Xing, Chaozhu,Zhang, Meng,Zhang, Jinfa,Zhang, Xuexian,Qi, Tingxiang,Guo, Liping,Wang, Hailin,Wu, Jianyong,Wu, Jianyong. 2017

[15]A High Density Slaf-Seq Snp G.enetic Map And Qtl F or Seed Size, Oil And Protein Content In Upland Cotton. Yang, Le,Wang, Wenwen,Teng, Zhonghua,Yang, Peng,Ali, Iftikhar,Zhang, Zhengsheng,Zhang, Jian,Sun, Ying,Liu, Dexin,Liu, Dajun,Guo, Kai,Liu, Fang,Ou, Yuncan,Ma, Junrui,Liu, Tianpeng,Cai, Xiaoyan. 2019

[16]Multi-Locus Genome-Wide Association Studies Of F.iber-Quality Related Traits In C hinese Early-Maturity Upland Cotton. Su, Junji,Su, Junji,Su, Junji,Ma, Qi,Wang, Caixiang,Su, Junji,Hao, Fushun,Li, Mei. 2018

[17]Gwas Analysis And Qtl Identification O.f Fiber Quality Traits A nd Yield Components In Upland Cotton Using Enriched High-Density Snp Markers. Pan, Jingtao,Xiao, Xianghui,Gong, Juwu,Deng, Xiaoying,Zhang, Qi,Jiang, Xiao,Duan, Li,Liu, Aiying,Zou, Xianyan,Hafeez, Abdul,Liu, Ruixian,Zhang, Zhen,Lu, Quanwei,Li, Junwen,Gong, Juwu,Yuan, Youlu,Wankui Gong,Lu, Quanwei,Liu, Ruixian,Xiao, Xianghui,Chen, Quanjia,Shi, Yuzhen,Shang, Haihong,Li, Shaoqi,Iqbal, Muhammad S.,Geng, Hongwei,Yuan, Youlu,Ge, Qun. 2018

[18]A Combined Small Rna And T.ranscriptome Sequencing Analysis Reveal R egulatory Roles Of Mirnas During Anther Development Of Upland Cotton Carrying Cytoplasmic Male Sterile Gossypium Harknessii (D2) Cytoplasm. Zhang, Xuexian,Zhang, Jinfa,Zhang, Meng,Zhang, Bingbing,Guo, Liping,Qi, Tingxiang,Liu, Guoyuan,Pei, Wenfeng,Wang, Hailin,Tang, Huini,Wu, Jianyong,Li, Xue,Shahzad, Kashif,Xing, Chaozhu,Qiao, Xiuqin. 2018

[19]Identifying Favorable Alleles For Improving K.ey Agronomic Traits In U pland Cotton. Cai, Yingfan,Miao, Yuchen,Wang, Liru,Pang, Baoyin,Jia, Yinhua,Wang, Mi,Sun, Junling,Pan, Zhaoe,He, Shoupu,Du, Xiongming,Dai, Panhong,Dai, Panhong. 2019

[20]Integrated Methylome And Transcriptome Analysis Between The Cms-D2 Line Zba And Its Maintainer Line Zb In Upland Cotton. Zhang, Xuexian,Zuo, Zhidan,Li, Ting,Wu, Jianyong,Shahzad, Kashif,Wang, Hailin,Feng, Juanjuan,Zhang, Bingbing,Qiao, Xiuqin,Tang, Huini,Guo, Liping,Qi, Tingxiang,Zhang, Meng,Xing, Chaozhu,Wu, Jianyong. 2019

作者其他论文 更多>>