数字农科院2.0

Automatic immunomagnetic beads clean-up coupled with HPLC-FLD for rapid, simple and high-throughput detection of zearalenone in cereal foods and feeds

文献类型： 外文期刊

作者： Zhiming Xiao;Jun Zhang;Yinji Jin;Bo He;Ting Yao;Jing Tian;Fan Mo;Decheng Suo;Shi Wang;Runxian Li;Lijie Zhang;Shen Hu;Xia Fan

作者机构：

关键词： Automation;Feedstuff;Foods;Immunomagnetic beads;Zearalenone

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2025 年 496 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： This study developed an automatic immunomagnetic beads (IMBs) clean-up coupled with high-performance liquid chromatography-fluorescence detector (HPLC-FLD) for rapid, simple and high-throughput detection of zearalenone (ZEN) in cereal foods and feeds. The method exhibited good linearity (R2 ≥ 0.9999) and sensitivity, with the limit of detection (LOD) and quantification (LOQ) of 0.3 μg/kg and 1.0 μg/kg, respectively. The mean recoveries of the spiked samples (1, 2, 10 and 100 μg/kg with ZEN) were 85.9 %–101.6 %, with intra-day relative standard deviation (RSD) in the ranges of 1.25 %–6.63 % and inter-day RSD between 3.60 % and 8.75 %. Compared with traditional solid-phase extraction and immunoaffinity column clean-up methods, this IMBs method integrates isolation, purification and concentration in a single step. Furthermore, up to 24 samples can undergo fully automated processing in 20 min, which significantly improved efficiency and avoided deviations caused by manual operation, making the approach more suitable for routine high-throughput surveillance.

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