数字农科院2.0

A sweet cherry AGAMOUS-LIKE transcription factor PavAGL15 affects fruit size by directly repressing the PavCYP78A9 expression

文献类型： 外文期刊

作者： Yuanxin Dong;Xiliang Qi;Congli Liu;Lulu Song;Li Ming

作者机构：

关键词： Fruit size;PavAGL15;PavCYP78A9;Sweet cherry;Virus-induced gene silencing (VIGS)

期刊名称： Scientia Horticulturae

ISSN： 0304-4238

年卷期： 2022 年 297 卷

页码：

收录情况： SCIE（2022版）

摘要： Fruit size, as an important fruit quality trait, is strongly associated with the commercial value of sweet cherry (Prunus avium L.). Understanding the genetic mechanisms underlying the control of fruit size is essential for breeding new varieties with larger fruit to increase yield and economic value. However, little is known about the molecular mechanisms underlying fruit size in sweet cherry. Here, we identified and functionally characterized a MIKC-type MADS-box transcription factor, PavAGL15, in sweet cherry, as a putative protein that binds the promoter of PavCYP78A9, a cytochrome P450, which controls sweet cherry fruit size. Yeast one-hybrid and transient dual-luciferase reporter assays indicated that PavAGL15 directly bound to the PavCYP78A9 promoter in vitro and in vivo, resulting in repressing expression. PavAGL15 was expressed at high levels in flower buds, blossoms and young fruit as assessed by real-time PCR. The PavAGL15 protein localized to the nucleus. Silencing PavAGL15 using virus-induced gene silencing significantly increased the fruit size, which caused the high expression levels of PavCYP78A9, cell cycling and proliferation-related genes in sweet cherry fruits. Collectively, these results demonstrate that PavAGL15 is a negative regulator of sweet cherry fruit size that regulates PavCYP78A9 expression at the transcriptional level. Thus, we have gained new insights into the regulatory networks affecting fruit size during sweet cherry fruit growth and development.

分类号：

  • 相关文献

[1]Sweet cherry AP2/ERF transcription factor, PavRAV2, negatively modulates fruit size by directly repressing PavKLUH expression. Xiliang Qi,Lifeng Liu,Congli Liu,Lulu Song,Yuanxin Dong,Lei Chen,Ming Li. 2023

[2]PacMYBA, a sweet cherry R2R3-MYB transcription factor, is a positive regulator of salt stress tolerance and pathogen resistance. Shen, Xinjie,Guo, Xinwei,Guo, Xiao,Zhao, Di,Li, Tianhong,Shen, Xinjie,Zhao, Wei,Chen, Jingsheng,Li, Tianhong.

[3]Outdoor color rating of sweet cherries using computer vision. Wang, Qi,Wang, Hui,Xie, Lijuan,Zhang, Qin,Wang, Hui,Xie, Lijuan.

[4]Role of abscisic acid and ethylene in sweet cherry fruit maturation: molecular aspects. Ren, J.,Chen, P.,Dai, S. J.,Li, P.,Li, Q.,Ji, K.,Wang, Y. P.,Leng, P.,Ren, J.. 2011

[5]Exogenous Melatonin Delays Postharvest Fruit S.enescence And Maintains The Q uality Of Sweet Cherries. Wang, Feng,Wang, Feng,Wang, Feng,Zhao, Qifeng,Zhang, Xiaoping,Yang, Qingzhen. 2019

[6]Molecular characterization of two isoforms of a farnesyl pyrophosphate synthase gene in wheat and their roles in sesquiterpene synthesis and inducible defence against aphid infestation. Li, Zhi-Xia,Yu, Xiu-Dao,Zhao, Guang-Yao,Xia, Lan-Qin,Zhang, Yan,Shi, Yi,Fan, Jia,Pickett, John A.,Jones, Huw D.,Zhou, Jing-Jiang,Birkett, Michael A.,Caulfield, John,Napier, Johnathan A.,Bruce, Toby J. A.,Cheng, Xian-Guo.

[7]Pacmyba, A Sweet Cherry R2R3-Myb T.ranscription Factor, Is A P ositive Regulator Of Salt Stress Tolerance And Pathogen Resistance. Shen, XJ, Guo, XW, Guo, X, Zhao, D, Zhao, W, Chen, JS, Li, TH. 2017

[8]Maize FERONIA-like receptor genes are involved in the response of multiple disease resistance in maize. Yu, Haiyue,Ruan, Hongchun,Xia, Xinyao,Chicowski, Aline Sartor,Whitham, Steven A.,Li, Zhiqiang,Wang, Guirong,Liu, Wende. 2022

[9]Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis. Gongmin Cheng,Mengdi Wang,Longyan Zhang,Hengling Wei,Hantao Wang,Jianhua Lu,Shuxun Yu. 2022

[10]A Sweet Cherry Glutathione S-Transferase Gene, PavGST1, Plays a Central Role in Fruit Skin Coloration. Xiliang Qi,Congli Liu,Lulu Song,Yuanxin Dong,Lei Chen,Ming Li. 2022

[11]A 5.2-kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase-like enhances fruit firmness in Prunus avium. Qi, Xiliang,Dong, Yuanxin,Liu, Congli,Song, Lulu,Chen, Lei,Li, Ming. 2024

[12]Physicochemical Properties of Geographical Indication (GI) Sweet Cherries in China and Their Influencing Factors of Cultivar, Climate Type, and Soil Condition. Ying Nie,Jiazhang Huang,Rui Liu,Pei Wang,Peng Liu,Man Lu,Junmao Sun. 2023

[13]Genome-wide identification and expression analysis of terpene synthases in Gossypium species in response to gossypol biosynthesis. Teame Gereziher Mehari,Hui Fang,Wenxiang Feng,Yuanyuan Zhang,Muhammad Jawad Umer,Jinlei Han,Allah Ditta,Muhammad K.R. Khan,Fang Liu,Kai Wang,Baohua Wang. 2023

[14]Genome-Wide Identification of the GhANN Gene Family and Functional Validation of GhANN11 and GhANN4 under Abiotic Stress. Jin Luo,Meili Li,Jisheng Ju,Han Hai,Wei Wei,Pingjie Ling,Dandan Li,Junji Su,Xianliang Zhang,Caixiang Wang. 2024

[15]GhBRX.1, GhBRX.2, and GhBRX4.3 improve resistance to salt and cold stress in upland cotton. Wei W.,Ju J.,Zhang X.,Ling P.,Luo J.,Li Y.,Xu W.,Su J.,Zhang X.,Wang C.. 2024

[16]Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress. Caixiang Wang,Meili Li,Dingguo Zhang,Xueli Zhang,Juanjuan Liu,Junji Su. 2024

[17]GhADT5 enhances alkali stress tolerance in cotton by regulating phenylalanine-derived flavonoid biosynthesis and antioxidant defense. Wang, Lidong,Nan, Hongyu,Zhang, Menghao,Guang, Lijun,Meng, Junting,Liu, Mengyue,Meng, Yuan,Chen, Wenhua,Fan, Yapeng,Huang, Hui,Sun, Yuping,Yang, Zhining,Chen, Xiao,Wu, Fange,Song, Ruize,Wang, Shuai,Lu, Xuke,Chen, Xiugui,Zhao, Lanjie,Wang, Junjuan,Cui, Yupeng,Zhou, Xue-Rong,Wang, Ning,Feng, Keyun,Chen, Qin,Ye, Wuwei. 2025

[18]Unveiling the Role of GhP5CS1 in Cotton Salt Stress Tolerance: A Comprehensive Genomic and Functional Analysis of P5CS Genes. Hui Fang,Xin Gao,Yunhao Wu,Ke Zhang,Ying Wu,Junyi Li,Dongmei Qian,Ruochen Li,Haijing Gu,Teame Gereziher Mehari,Xinlian Shen,Baohua Wang. 2025

[19]Malate accumulation and transcriptome patterns during fruit development in sweet Cherry (Prunus avium L.). Congli Liu,Lei Chen,Xiliang Qi,Lulu Song,Manqing Wang,Shu Han,Ming Li. 2025

[20]Effected Cultural Practices on Fruit Quality and Polyphenols Content. Khanizadeh, S.,Charles, M. T.,Fan, L.,Yu, C.,Tao, S.,Fan, L.. 2011

作者其他论文 更多>>