数字农科院2.0

Preparation of monoclonal antibodies recognizing pharmacologically active metabolites of metamizole based on rational hapten design and their application in the detection of animal-derived food

文献类型： 外文期刊

作者： Linwei Zhang;Jiacan Wang;Yiting Wang;Hao Wen;Mingyue Ding;Jiaxu Xiao;Hongfei Yang;Xiaoming Pan;Shiyun Han;Dapeng Peng

作者机构：

关键词： （0-1-3）Hapten design;ic-ELISA;Metamizole;Monoclonal antibody

期刊名称： Talanta

ISSN： 0039-9140

年卷期： 2024 年 280 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Metamizole (MET) is an antipyretic and analgesic drug, the illegal use of which can result in residues of MET metabolites in edible tissues of animals. In this study, a computational chemistry-assisted hapten screening strategy was used to screen for the optimal immunogenic hapten-A and the optimal coating antigen hapten-G-OVA. A monoclonal antibody capable of recognizing two pharmacologically active metabolites of MET, 4-methylamidinoantipyrine (MAA) and 4-aminoantipyrine (AA), was prepared from the hapten-A. The antibody showed excellent specificity for MAA and AA and almost no cross-reactivity with the pharmacologically inactive metabolites 4-formamidinoantipyrine (FAA) and 4-acetamidinoantipyrine (AAA). An ic-ELISA was developed for the simultaneous detection of MAA and AA in animal-derived food, the limits of detection for MAA ranged from 0.93 to 1.18 μg/kg, while those for AA ranged from 1.74 to 4.61 μg/kg. The recovery rate fell within the range of 82 %–110 %, with a coefficient of variation less than 16.39 %.

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