数字农科院2.0

Anti-Diabetic Effect of Soy–Whey Dual-Protein on Mice with Type 2 Diabetes Mellitus Through INS/IRS1/PI3K Signaling Pathway

文献类型： 外文期刊

作者： Na Li;Hu Li;Duo Feng;Mengjie Li;Di Han;Tianxin Liu;Jing Wang

作者机构：

关键词： dual-protein;gut microbiota;insulin resistance;mechanism of action;type 2 diabetes

期刊名称： Foods

ISSN： 2304-8158

年卷期： 2025 年 14 卷 12 期

页码：

收录情况： SCIE（2025版）

摘要： The effects of soy protein and whey protein supplementation on glycemic control show inconsistency, and the mechanisms underlying the impact of a high-protein diet on blood glucose regulation remain unclear. This study aimed to explore the impact of a dual-protein (DP) blend comprising soy protein isolate (SPI) and whey protein concentrate (WPC), processed through high-pressure homogenization, on mice with Type 2 diabetes mellitus (T2DM) and its potential mechanisms. In the in vitro experiments, an insulin-resistant (IR) HepG2 cell model was treated with DP, resulting in a significant enhancement of glucose uptake and upregulation of IRS1 and GLUT4 expression. For the in vivo experiments, male C57BL/6J mice were randomly assigned into four groups (n = 6) based on body weight: normal control, T2DM model group, Metformin-treated group, and DP-treated group. Following a 5-week feeding period, Metformin and DP significantly reduced levels of blood sugar, AUC, TC, TG, and LDL-C in T2DM mice. Additionally, TP and ALB levels in the DP group were notably higher in the model group. In the liver and pancreas, DP alleviated histopathological changes and promoted liver glycogen synthesis in T2DM mice. Moreover, the levels of IRS1 and PI3K in the livers of mice in the DP group were significantly higher than those in the model group. Compared with the model groups, DP significantly reduced the expression of CD45 and increased the expression of CD206 in the pancreas of mice. Furthermore, 16S rRNA analysis revealed that DP altered the composition of the gut microbiota in diabetic mice, increasing the relative abundance of Lactobacillus, Parvibacter, and Lactobacillaceae. This suggested that DP could alleviate functional metabolic disorders in the gut and potentially reverse the risk of related complications. In conclusion, soy whey dual-protein may have an effective nutritional therapeutic effect on T2DM mice by regulating lipid metabolism, the INS/IRS1/PI3K signaling pathway, and gut microbiota.

分类号：

  • 相关文献

[1]Low glycemic index potato biscuits alleviate physio-histological damage and gut dysbiosis in rats with type-2 diabetes mellitus induced by high-sugar and high-fat diet and streptozotocin. Renjie Zhao,Nan Li,Wei Liu,Qiannan Liu,Liang Zhang,Xingyun Peng,Ruixuan Zhao,Honghai Hu. 2023

[2]Alleviating effects of polyphenol extract from rapeseed meal on type 2 diabetes in mice via modulation of gut microbiota and AMPK/mTOR signaling pathways. Xiaoyang Xia,Zhen Zhang,Zhenxia Xu,Chi Zeng,Xia Xiang,Fenghong Huang. 2025

[3]Dietary fiber of Tartary buckwheat bran modified by steam explosion alleviates hyperglycemia and modulates gut microbiota in db/db mice. Xiaoqin He,Weizhou Li,Yuanyuan Chen,Lin Lei,Fuhua Li,Jichun Zhao,Kaifang Zeng,Jian Ming. 2022

[4]The Effect of High Pressure Homogenization on the Structure of Dual-Protein and Its Emulsion Functional Properties. Meishan Wu,Xiaoye He,Duo Feng,Hu Li,Di Han,Qingye Li,Boya Zhao,Na Li,Tianxin Liu,Jing Wang. 2023

[5]Synergistic construction of high-performance oil-in-water emulsions by egg white protein-whey protein isolate dual-protein: achieving efficient utilization of bioactive substances. Yanli Wang,Yuanyuan Zhang,Erjiao Li,Haobo Jin,Yaqin Yang,Sadaqat Ali,Qun Lu,Zhaoxia Cai,Guofeng Jin,Long Sheng. 2025

[6]EFFECT OF RAW CAMEL MILK IN TYPE 2 DIABETES ANIMAL MODELS AND PATIENTS: TEN MONTHS RANDOMISED STUDY. Wang, S. Y.,Shao, W. J.,Heng, H.,Wang, S. Y.,Liang, J. P.,Song, N. N.. 2009

[7]Baicalin suppresses the progression of Type 2 diabetes-induced liver tumor through regulating METTL3/m6A/HKDC1 axis and downstream p-JAK2/STAT1/clevaged Capase3 pathway. Hongpeng Jiang,Qianqian Yao,Yongbo An,Linlin Fan,Jing Wang,Huiying Li. 2022

[8]Effect of different drying techniques on structural characteristics and bioactivities of polysaccharides extracted from (Lithocarpus litseifolius [Hance] Chun) sweet tea leaves. Guo, Huan,Fu, Meng-Xi,Zhao, Yun-Xuan,Wu, Ding-Tao,Liu, Hong-Yan,Li, Hua-Bin,Ayyash, Mutamed,Gan, Ren-You. 2022

[9]Research Progress on the Effect and Mechanism of Tea Products with Different Fermentation Degrees in Regulating Type 2 Diabetes Mellitus. Li G.,Zhang J.,Cui H.,Feng Z.,Gao Y.,Wang Y.,Chen J.,Xu Y.,Niu D.,Yin J.. 2024

[10]Meat products consumption and risk for Alzheimer’s dementia and type 2 diabetes: a systematic review and meta-analysis of cohort studies. Mingyu Li,Xiaoyue Yang,Lu Sun,Hongwei Xiao,Chongxin Liu,Chunjiang Zhang,Zhenyu Wang,Christophe Blecker,Dequan Zhang. 2025

[11]Alleviating effects of polyphenol extract from rapeseed meal on type 2 diabetes in mice via modulation of gut microbiota and AMPK/mTOR signaling pathways. Xiaoyang Xia,Zhen Zhang,Zhenxia Xu,Chi Zeng,Xia Xiang,Fenghong Huang. 2024

[12]Inflammation-mediated effects of diabetes mellitus on male fertility: a systematic review and meta-analysis. Hao, Yanan,Yang, Zheng,Feng, Yanni,Zhao, Yong,Ren, Yonglin. 2025

[13]The toxicity and the acaricidal mechanism against Psoroptes cuniculi of the methanol extract of Adonis coerulea Maxim. Shang, Xiaofei,Guo, Xiao,Yang, Feng,Li, Bing,Pan, Hu,Miao, Xiaolou,Zhang, Jiyu.

[14]Antifungal Activity of Citrus Essential Oils. Jing, Li,Li, Ligai,Xi, Wanpeng,Zhou, Zhiqin,Jing, Li,Li, Ligai,Xi, Wanpeng,Zhou, Zhiqin,Jing, Li,Lei, Zhentian,Sumner, Lloyd W.,Xie, Rangjin,Guan, Yu.

[15]New insights into membrane-active action in plasma membrane of fungal hyphae by the lipopeptide antibiotic bacillomycin L. Zhang, Bao,Han, Yuzhu,Li, Pinglan,Dong, Chunjuan,Shang, Qingmao.

[16]Bacterial resistance to antibacterial agents: Mechanisms, control strategies, and implications for global health. Li, Ting,Wang, Zhenlong,Guo, Jianhua,de la Fuente-Nunez, Cesar,Wang, Jinquan,Han, Bing,Tao, Hui,Liu, Jie,Wang, Xiumin. 2023

[17]Short peptides make a big difference: the role of botany-derived amps in disease control and protection of human health. Xiumei Luo,Wenxian Wu,Li Feng,Haim Treves,Maozhi Ren. 2021

[18]Elderberry (Sambucus nigra L.): Bioactive Compounds, Health Functions, and Applications. Dan Liu,Xiao Qin He,Ding Tao Wu,Hua Bin Li,Yi Bin Feng,Liang Zou,Ren You Gan. 2022

[19]Developments in the study of Chinese herbal medicine's assessment index and action mechanism for diabetes mellitus. Liu, Xin-Yue,Zheng, Han-Wen,Wang, Feng-Zhong,Atia, Tul-Wahab,Fan, Bei,Wang, Qiong. 2024

[20]In Vitro/Vivo Mechanisms of Antibacterial Peptide NZ2114 against Staphylococcus pseudintermedius and Its Biofilms. Shuang Zhang,Na Yang,Ruoyu Mao,Ya Hao,Da Teng,Jianhua Wang. 2024

作者其他论文 更多>>