数字农科院2.0

Association analysis of single nucleotide polymorphisms in the LIFR gene with lambing number in sheep

文献类型： 外文期刊

作者： Yuliang Wen;Yuping Xiang;Runan Zhang;Kai Liu;Yufang Liu;Mingxing Chu

作者机构：

关键词： lambing number;LIFR;MassARRAY;molecular marker;sheep

期刊名称： Frontiers in Veterinary Science

ISSN： 2297-1769

年卷期： 2025 年 12 卷

页码：

收录情况： SCIE（2025版）

摘要： Lambing number trait in sheep is a complex trait controlled by multiple genes, and low lambing number has a severe economic impact on the sheep industry. Previous studies have shown that Sparc/Osteonectin, cwcv, and kazal-like domain proteoglycan 1 (SPOCK1), A disintegrin and metalloproteinase with thrombospondin-like repeats 1 (ADAMTS1), heparin-binding epidermal growth factor-like growth factor (HBEGF), and leukemia inhibitory factor receptor (LIFR) are involved in mammalian reproduction. However, the effects of these genes on lambing number in sheep are still unclear. In this study, single nucleotide polymorphism (SNP) loci at the above four genes were genotyped in five sheep breeds (two single-born sheep breeds and three multiple-born sheep breeds with a total of 768 sheep) using the Sequenom MassARRAY® SNP assay, and their associations with the lambing number in small-tailed Han sheep were analyzed. The results showed that a total of six SNP loci (c.*1633A>G, c.*1388T>C, c.*1095G>A, c.1847A>G, c.*2403A>G, and c.*127T>C) were identified in the four genes, SPOCK1, ADAMTS1, HBEGF, and LIFR. All the above SNPs had three genotypes in five sheep breeds. The population genetic analysis of SNPs of the four genes and the association analysis with lambing number showed that the polymorphism information content of the five sheep breeds of small-tailed Han sheep, Hu sheep, Cele black sheep, Sunite sheep, and Bamei mutton sheep was between 0 and 0.37, and a few sheep breeds were in Hardy-Weinberg equilibrium (p>0.05). The c.*127 T>C locus of the LIFR gene may be affected by natural or artificial selection in these sheep breeds. Additionally, the association analysis between the c.*127 T>C locus of LIFR gene and the lambing number of small-tailed Han sheep showed that the c.*127 T>C locus of the LIFR gene was significantly associated with the lambing number of the second, third, and average parity of smalltailed Han sheep (p < 0.05). The lambing number of ewes with the LIFR CC genotype was significantly lower than that with the TT and CT genotypes (p < 0.05). The LIFR protein interaction network was also predicted and found to interact with the reported ciliary neurotrophic factor (CNTF), cardiotrophinlike cytokine factor 1 (CLCF1), interleukin 6 cytokine family signal transducer (IL6ST), and ciliary neurotrophic factor receptor (CNTFR) proteins. In conclusion, the c.*127 T>C locus of LIFR gene can be used as a candidate genetic molecular marker to increase lambing numbers in polytocous sheep.

分类号：

  • 相关文献

[1]Mutation of the ETS1 3′UTR interacts with miR-216a-3p to regulate granulosa cell apoptosis in sheep. Fan Yang,Yufang Liu,Peng Wang,Xiangyu Wang,Mingxing Chu,Pingqing Wang. 2023

[2]Mutation of the ETS1 3 & PRIME;UTR interacts with miR-216a-3p to regulate granulosa cell apoptosis in sheep. Yang, Fan,Liu, Yufang,Wang, Peng,Wang, Xiangyu,Chu, Mingxing,Wang, Pingqing. 2023

[3]Association of VEGF and IGF2 Polymorphisms with Lambing Number of Small Tail Han Sheep. Peng Wang,Cai Xia Geng,Bao Yun Zhang,Hong Quan Chen,Ping Qing Wang,Yu Fang Liu,Ming Xing Chu. 2025

[4]Association Analysis of SNPs in GRHL2 and RORA Genes with Lambing Number in Small-Tailed Han Sheep. Pu, Xiufen,Liu, Kai,Wang, Xiangyu,Di, Ran,He, Xiaoyun,Liu, Yufang,Chu, Mingxing. 2025

[5]Screening of Litter-Size-Associated SNPs in NOX4, PDE11A and GHR Genes of Sheep. Jiajun Li,Yiming Gong,Xiangyu Wang,Xiaoyun He,Xiaolong He,Mingxing Chu,Ran Di. 2024

[6]赖氨酸对绵羊GHR基因表达的影响(英文). 李建升,程胜利,韩向敏,冯瑞林,岳耀敬,杨博辉,刘建斌. 2009

[7]基于ssRNA-seq研究差异基因、NATs对藏羊高原低氧适应的调控. 岳耀敬,冯瑞林,王天翔,郭婷婷,李桂英,孙晓萍,刘建斌,李文辉,牛春娥,郭健,李范文,杨博辉. 2015

[8]A intervarietal genetic map and QTL analysis for yield traits in wheat. Li, Sishen,Jia, Jizeng,Wei, Xianyun,Zhang, Xiaocun,Li, Linzhi,Chen, Haimei,Fan, Yuding,Sun, Haiyan,Zhao, Xinhua,Lei, Tiandong,Xu, Yunfong,Jiang, Fangshan,Wang, Honggang,Li, Lihui. 2007

[9]Analysis of QTL for resistance to head smut (Sporisorium rediana). Li, X. H.,Gao, S. R.,Shi, H. L.,Zhang, S. H.,Li, M. S.,Xie, C. X.,Wang, Z. H.,George, M. L. C.. 2008

[10]AFLP-Based Genetic Diversity among the Populations of Rosa laxa in Tianshan Mountains of Xinjiang, China. Yang, S. H.,Guo, N.,Ge, W. Y.,Ge, H.. 2013

[11]Genome-Wide Linkage Mapping of QTL for Yield Components, Plant Height and Yield-Related Physiological Traits in the Chinese Wheat Cross Zhou 8425B/Chinese Spring. Gao, Fengmei,Wu, Xiaoxia,Gao, Fengmei,Wen, Weie,Liu, Jindong,Rasheed, Awais,Xia, Xianchun,He, Zhonghu,Gao, Fengmei,Rasheed, Awais,He, Zhonghu,Yin, Guihong. 2015

[12]Confirmation of a major QTL on chromosome 10 for maize kernel row number in different environments. Tian, Baohua,Wang, Jianhua,Wang, Guoying. 2014

[13]Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars. Ren Xiao-li,Liu Tai-guo,Liu Bo,Gao Li,Chen Wan-quan. 2015

[14]Distribution of the Rht-B1b, Rht-D1b and Rht8 reduced height genes in autumn-sown Chinese wheats detected by molecular markers. Zhang, Xiaoke,Yang, Songjie,Zhou, Yang,He, Zhonghu,Xia, Xianchun. 2006

[15]Increasing Maize Productivity in China by Planting Hybrids with Germplasm that Responds Favorably to Higher Planting Densities. Li, Yu,Ma, Xinglin,Wang, Tianyu,Li, Yongxiao,Liu, Zhizhai,Shi, Yunsu,Song, Yanchun,Sun, Shuku,Liu, Cheng,Sun, Baocheng,Niebur, William,Carlone, Mario,Whitaker, David,Wilson, William,Smith, Stephen.

[16]Molecular markers for tracking variation in lipoxygenase activity in wheat breeding. Geng, Hongwei,Zhang, Yan,He, Zhonghu,Xia, Xianchun,Geng, Hongwei,Qu, Yanying,He, Zhonghu,Zhang, Liping,Appels, Rudi.

[17]Fine mapping of qSTV11(KAS), a major QTL for rice stripe disease resistance. Zhang, Ying-Xin,Wang, Qi,Jiang, Ling,Liu, Ling-Long,Wang, Bao-Xiang,Shen, Ying-Yue,Cheng, Xia-Nian,Wan, Jian-min,Wan, Jian-min.

[18]An ISSR-based Approach for the Molecular Detection and Diagnosis of Dwarf Bunt of Wheat, Caused by Tilletia controversa Kuhn. Gao, Li,Chen, Wanquan,Liu, Taiguo.

[19]Development and characterization of simple sequence repeat (SSR) markers for the mushroom Flammulina velutipes. Zhang, Ruiying,Wang, Hexiang,Zhang, Ruiying,Wang, Hexiang,Zhang, Ruiying,Zhang, Jinxia,Zuo, Xuemei,Jiang, Ruibo,Hu, Dandan,Ng, Tzi Bun.

[20]Identification of SRAP markers linked to the restorer gene of CMS7311 in heading Chinese cabbage [Brassica rapa L. subsp pekinensis (Lour.) Olsson]. Xu, X.,Zhang, Y.,Hui, M.,Zhang, M.,Zhang, L.,Xu, X.,Zhang, Y.,Hui, M.,Zhang, M.,Zhang, L.,Fang, Z.. 2016

作者其他论文 更多>>