数字农科院2.0

CO2 agitation combined with magnetized biochar to alleviate ammonia inhibited steady-state: Exploring the mechanism by combining metagenomics with macroscopic indicators

文献类型： 外文期刊

作者： Yu, Jiadong;Usman, Muhammad;Liu, Fan;Schafer, Franziska;Shen, Yuhan;Zheng, Zehui;Cai, Yafan

作者机构：

关键词： Ammonia inhibited steady-state;Anaerobic digestion;Biochar;Direct interspecies electron transfer (DIET);CO2 agitation

期刊名称： WATER RESEARCH

ISSN： 0043-1354

年卷期： 2025 年 276 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The ammonia inhibited steady-state phenomenon is frequently observed in the anaerobic digestion (AD) process of nitrogen-rich substrates. Reconfiguring microbial ecosystems has proven to be an effective strategy for mitigating ammonia inhibition. In the current study, biochars were screened and targeted for modification. CO2 agitation combined with magnetized biochar was used to aid the semi-continuous AD systems with ammonia inhibited steady-state. The results indicated that coconut shell biochar had the best stimulating effect on AD performance. The content of oxygen-containing functional groups (OCFGs), which had a positive correlation with the electron donating capacity (EDC), was targeted to be regulated. This strategy significantly increased the CH4 yield by 31.7 % (from 344 to 278 mL/g VS) (p < 0.05). Isotope tracing and KEGG gene annotation indicated that this strategy stimulated the efficiency of the hydrogenotrophic pathway. Simultaneously, it accelerated the attachment of microorganisms, which made the DIET pathway between bacteria and archaea efficient. Under CO2 agitation, the attachment of functional microorganisms to the biochar accelerated. Biochar weakened the synthesis of bioelectronic carriers (Cyt-c and chemosensory pili), while the electroactivity of the AD system was enhanced. This means that biochar replaced bioelectronic carriers and improved the DIET efficiency. In addition, the strategy had a positive effect on the colonization of simultaneous nitrification-denitrifying bacteria (Georgenia), which led to a decrease in ammonia nitrogen concentrations. This study revealed the mechanism by which this strategy alleviates ammonia inhibition and provided a promising strategy for the efficient AD of nitrogen-rich substrates.

分类号：

  • 相关文献

[1]Biochar accelerates methane production efficiency from Baijiu wastewater: Some viewpoints considering direct interspecies electron transfer. Yun He,Shilei Wang,Caihong Shen,Zhi Wang,Yiyang Liu,Xingyao Meng,Xiaoyong Li,Xiaoling Zhao,Jinmeng Chen,Jingliang Xu,Jiadong Yu,Yafan Cai,Hanjie Ying. 2024

[2]Bio-based carbon materials with multiple functional groups and graphene structure to boost methane production from ethanol anaerobic digestion. Yeqing Li,Zhenxin Wang,Zhuoliang Jiang,Lu Feng,Junting Pan,Mingyu Zhu,Chengjie Ma,Zhangmu Jing,Hao Jiang,Hongjun Zhou,Hui Sun,Hongbin Liu. 2022

[3]Tree-based machine learning model for visualizing complex relationships between biochar properties and anaerobic digestion. Yi Zhang,Yijing Feng,Zhonghao Ren,Runguo Zuo,Tianhui Zhang,Yeqing Li,Yajing Wang,Zhiyang Liu,Ziyan Sun,Yongming Han,Lu Feng,Mortaza Aghbashlo,Meisam Tabatabaei,Junting Pan. 2023

[4]The role of electrochemical properties of biochar to promote methane production in anaerobic digestion. Ziyan Sun,Lu Feng,Yeqing Li,Yongming Han,Hongjun Zhou,Junting Pan. 2022

[5]Response of food waste anaerobic digestion to the dimensions of micron-biochar under 30 g VS/L organic loading rate: Focus on gas production and microbial community structure. Qiang Liu,Ziyan Sun,Junting Pan,Lu Feng,Hongjun Zhou,Yeqing Li,Gang Li. 2024

[6]A novel strategy for enhancing electron transfer by adding BC/AQS in the anaerobic digestion of agriculture waste: Focusing on the interaction between quinone/C=O and microbes. Yulei Zhang,Zonglu Yao,Junyi Ma,Jiadong Yu,Ping Ai,Ruixia Shen,Yi Liang,Lixin Zhao. 2025

[7]Modified biochar effectively removes tetracycline and antibiotic resistance genes to enhance anaerobic digestion performance. Chuan Ding,Jiahao Liu,Jinghui Wang,Jiayu Le,Beini Lu,Nannan Qu,Shenggeng Zhao,Haoyu Zhang,Jianping Su,Yeqing Li,Qiang Liu. 2025

[8]Building a knowledge-based machine learning loop framework to optimize biochar for anaerobic digestion performance. Yi Zhang,Yu Fu,Zhonghao Ren,Yeqing Li,Yijing Feng,Zheng Hao Leong,Junting Pan. 2025

[9]Biochar reduces nitrogen leaching of digestate field application by inhibiting nitrification and enhancing nitrate conversion. Xiaofeng Liang,Hailin Tian,Peiyu Feng,Dongdong Zhang,Miao Yan,Dandan Gao,Wenxia Tan,Qian Tan. 2025

[10]Performance evaluation of a novel anaerobic digestion operation process for treating high-solids content chicken manure: Effect of reduction of the hydraulic retention time at a constant organic loading rate. Zhang, Wanqin,Dong, Hongmin,Zhang, Wanqin,Dong, Renjie,Lang, Qianqian,Pan, Zhendong,Jiang, Yingqing,Liebetrau, Jan,Nelles, Michael.

[11]Efficient anaerobic digestion of whole microalgae and lipid-extracted microalgae residues for methane energy production. Zhao, Baisuo,Ma, Jingwei,Zhao, Quanbao,Chen, Shulin,Frear, Craig,Laurens, Lieve,Jarvis, Eric,Zhao, Baisuo.

[12]Performance of thermal pretreatment and mesophilic fermentation system on pathogen inactivation and biogas production of faecal sludge: Initial laboratory results. Yin, Fubin,Dong, Hongmin,Li, Zifu,Wang, Dongling,Ohlsen, Thomas.

[13]Effects of anaerobic digestion on chlortetracycline and oxytetracycline degradation efficiency for swine manure. Yin, Fubin,Dong, Hongmin,Tao, Xiuping,Chen, Yongxing,Ji, Chao.

[14]Combined effect of crude fat content and initial substrate concentration on batch anaerobic digestion characteristics of food waste. Zhang, Wanqin,Dong, Hongmin,Zhang, Wanqin,Li, Xin,Dong, Renjie,Lang, Qianqian,Fang, Ming,Bah, Hamidou.

[15]Enhancing anaerobic digestion of cotton stalk by pretreatment with a microbial consortium (MC1). Xufeng Yuan,Lei Ma,Boting Wen,Dayun Zhou,Meng Kuang,Weihua Yang,Zongjun Cui.

[16]Co-digestion of tobacco waste with different agricultural biomass feedstocks and the inhibition of tobacco viruses by anaerobic digestion. Liu, Yi,Liu, Gangjin,Yang, Hongnan,Wang, Lan,Kong, Chuixue,Zheng, Dan,Deng, Liangwei,Dong, Jianxin,Liu, Wei,Yang, Jinguang,Wang, Shusheng.

[17]Performance Evaluation Of A Novel A.naerobic Digestion Operation Process F or Treating High-Solids Content Chicken Manure: Effect Of Reduction Of The Hydraulic Retention Time At A Constant Organic Loading Rate. Zhang, WQ, Lang, QQ, Pan, ZD, Jiang, YQ, Liebetrau, J, Nelles, M, Dong, HM, Dong, RJ. 2017

[18]Methanogenic Community During The Anaerobic D.igestion Of Different Substrates A nd Organic Loading Rates. Kong, DW, Zhang, KQ, Liang, JF, Gao, WX, Du, LZ. 2019

[19]Effect Of Organic Loading Rate O.n Anaerobic Digestion Of P ig Manure: Methane Production, Mass Flow, Reactor Scale And Heating Scenarios. Duan, N, Zhang, DJ, Lin, C, Zhang, YF, Zhao, LY, Liu, HB, Liu, ZD. 2019

[20]Comparison Of Three Biomass-Retaining Reactors O.f The Asbr, The U bf And The Usr Treating Swine Wastewater For Biogas Production. Yang, HN, Deng, LW, Wang, L, Zheng, D, Liu, Y, Wang, S, Huang, FY. 2019

作者其他论文 更多>>