数字农科院2.0

GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.)

文献类型: 外文期刊

作者: Chen B.;Tian Z.;Fu G.;Zhang A.;Sun Y.;Wang J.;Pan Z.;Li H.;Hu D.;Xia Y.;He S.;Du X.

作者机构:

关键词: Cytoskeleton;Fiber length;GhWDL3;Microtubule-associated proteins (MAPs);TPX2;Upland cotton

期刊名称: Journal of Cotton Research

ISSN: 2096-5044

年卷期: 2024 年 7 卷 1 期

页码:

收录情况: 农林核心(2020版)

摘要: Background: Cotton fiber is a model tissue for studying microtubule-associated proteins (MAPs). The Xklp2 (TPX2) proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule (MT). However, there is a lack of studies concerning the systematic characterization of the TPX2 genes family in cotton. Therefore, the identification and portrayal of G. hirsutum TPX2 genes can provide key targets for molecular manipulation in the breeding of cotton fiber improvement. Result: In this study, TPX2 family genes were classified into two distinct subclasses TPXLs and MAP genes WAVE DAMPENED2-LIKE (WDLs) and quite conservative in quantity. GhWDL3 was significantly up-regulated in 15 days post anthesis fibers of ZRI-015 (an upland cotton with longer and stronger fiber). GhWDL3 promotes all stem hairs to become straight when overexpressed in Arabidopsis, which may indirectly regulate cotton fiber cell morphology during fiber development. Virus induced gene silencing (VIGS) results showed that GhWDL3 inhibited fiber cell elongation at fiber development periods through regulating the expression of cell wall related genes. Conclusion: These results reveal that GhWDL3 regulated cotton fiber cell elongation and provide crucial information for the further investigation in the regulatory mechanisms/networks of cotton fiber length. © The Author(s) 2024.

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