数字农科院2.0

Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents

文献类型： 外文期刊

作者： Yu Li;Yuqian Qiao;Yuxuan Zhu;Wangyang Shen;Weiping Jin;Dengfeng Peng;Qingrong Huang

作者机构：

关键词： Extraction;Oil bodies;Oleosin;Organic solvents;Protein assembly

期刊名称： Food Chemistry: X

ISSN： 2590-1575

年卷期： 2025 年 25 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： During the extraction of membrane proteins from oil bodies (OBs), organic solvents dissolve the lipid core and precipitate proteins through solvent stress. Here the effects of solvent type and defatting sequence on the composition and structure of membrane proteins were investigated via SDS-PAGE, FTIR, and SEM-EDS. High purity oleosin (86 %) was obtained by treatment first with a Floch solution and then with cold acetone and petroleum ether after twice washing OBs with urea. The 3D spatial structure of oleosin was predicted using AlphaFold 2, revealing that the secondary structure of oleosin was dominated by α-helices (>60 %). Oleosin consisted of two district types, with oleosin-H (16–17 kDa) being the part of the molecule with limited water solubility, while oleosin-L (13–14 kDa) constituted the non-soluble part. The results provided a technical means of efficient extraction of Camellia oleosins and selective separation of oleosin-L and oleosin-H.

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