Self-Assembling E2-Based Nanoparticles Improve Vaccine Thermostability and Protective Immunity against CSFV
文献类型： 外文期刊
作者： Song H.;Abdullah S.W.;Pei C.;Shi X.;Chen X.;Ma Y.;Yin S.;Sun S.;Huang Y.;Guo H.
作者机构：
关键词： CSFV;E2;nanoparticles;protective immunity;thermostability;vaccine
期刊名称： International Journal of Molecular Sciences
ISSN： 1661-6596
年卷期： 2024 年 25 卷 1 期
页码：
收录情况： SCIE（2024版）
摘要： Classical swine fever virus (CSFV) is a highly contagious pathogen causing significant economic losses in the swine industry. Conventional inactivated or attenuated live vaccines for classical swine fever (CSF) are effective but face biosafety concerns and cannot distinguish vaccinated animals from those infected with the field virus, complicating CSF eradication efforts. It is noteworthy that nanoparticle (NP)-based vaccines resemble natural viruses in size and antigen structure, and offer an alternative tool to circumvent these limitations. In this study, we developed an innovative vaccine delivery scaffold utilizing self-assembled mi3 NPs, which form stable structures carrying the CSFV E2 glycoprotein. The expressed yeast E2-fused protein (E2-mi3 NPs) exhibited robust thermostability (25 to 70 °C) and long-term storage stability at room temperature (25 °C). Interestingly, E2-mi3 NPs made with this technology elicited enhanced antigen uptake by RAW264.7 cells. In a rabbit model, the E2-mi3 NP vaccine against CSFV markedly increased CSFV-specific neutralizing antibody titers. Importantly, it conferred complete protection in rabbits challenged with the C-strain of CSFV. Furthermore, we also found that the E2-mi3 NP vaccines triggered stronger cellular (T-lymphocyte proliferation, CD8+ T-lymphocytes, IFN-γ, IL-2, and IL-12p70) and humoral (CSFV-specific neutralizing antibodies, CD4+ T-lymphocytes, and IL-4) immune responses in pigs than the E2 vaccines. To sum up, these structure-based, self-assembled mi3 NPs provide valuable insights for novel antiviral strategies against the constantly infectious agents. © 2024 by the authors.
分类号：
- 相关文献
作者其他论文 更多>>
-
Genetic control of thermomorphogenesis in tomato inflorescences
作者：Sun S.;Liu Z.;Wang X.;Song J.;Fang S.;Kong J.;Li R.;Wang H.;Cui X.
关键词：
-
A survey of efficient fine-tuning methods for Vision-Language Models — Prompt and Adapter
作者：Xing J.;Liu J.;Wang J.;Sun L.;Chen X.;Gu X.;Wang Y.
关键词：Adapter;Computer vision;Efficient fine-tuning;Pre-training model;Prompt;Vision-language
-
GhLCYε-3 characterized as a lycopene cyclase gene responding to drought stress in cotton
作者：Ni K.;Lu X.;Li S.;Li F.;Zhang Y.;Cui R.;Fan Y.;Huang H.;Chen X.;Wang J.;Wang S.;Guo L.;Zhao L.;He Y.;Ye W.
关键词：Carotenoids;Cotton;Functional verification;Lutein;Lycopene cyclase;Reactive oxygen species (ROS)
-
Tembusu virus induced apoptosis in vacuolate spermatogenic cells is mediated by Cytc-mediated mitochondrial apoptotic signaling pathway
作者：Huang Y.;Zhang Y.;Yang S.;Shi Y.;Chu X.;Ahmed N.;Wu J.;Chen Q.
关键词：Apoptosis;Cytochrome c;Duck tembusu virus;Spermatogenic cells;Testis
-
A strategy for modelling sediment distribution along slope lengths of an ephemeral gully through the simulated scouring experiments
作者：Huang Y.;Huang Y.;Zhuang X.;Wang W.;Zhang Q.;Dong Y.
关键词：Ephemeral gully;Sediment distribution;Sediment transport capacity;Simulation model;Soil erosion
-
Transcriptome Analysis of LLC-PK Cells Single or Coinfected with Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus
作者：Lu Y.;Yu R.;Tong L.;Zhang L.;Zhang Z.;Pan L.;Wang Y.;Guo H.;Hu Y.;Liu X.
关键词：coinfection;differential transcriptomics;interferon-stimulated genes (ISGs);porcine deltacoronavirus (PDCoV);porcine epidemic diarrhea virus (PEDV)
-
High internal phase Pickering emulsions stabilized by the complexes of ultrasound-treated pea protein isolate/mung bean starch for delivery of β-carotene
作者：Li S.;Zhu Y.;Hao X.;Su H.;Chen X.;Yao Y.
关键词：High internal phase Pickering emulsions;Mung bean starch;Pea protein isolate;Ultrasound;β-Carotene delivery