数字农科院2.0

Discovery of a Structurally Stable Immunodominant Region in ASFV p30 C-Terminus Using a Panel of Monoclonal Antibodies

文献类型： 外文期刊

作者： Wei Liu;Shandian Gao;Jiaoyan Su;Jinshu Sui;Tong Zhou;Jian Yang;Haiyan Lu;Huichen Guo;Junjun Shao;Huiyun Chang

作者机构：

关键词： African swine fever virus;epitope mapping;immunodominant region;monoclonal antibodies;p30

期刊名称： Transboundary and Emerging Diseases

ISSN： 1865-1674

年卷期： 2025 年 2025 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： African swine fever (ASF) is a lethal hemorrhagic viral disease of pigs with devastating global socioeconomic impacts. Due to the absence of a safe and effective vaccine, early surveillance and precise diagnostics are critical for ASF prevention and control. The ASF virus (ASFV) p30 protein, one of the most immunogenic early-expressed antigens during infection, is a prime target for diagnostic assays and subunit vaccines. Its comprehensive antigenic characterization remains crucial for rational vaccine design. In this study, we generated a panel of 20 monoclonal antibodies (MAbs) against the ASFV p30 protein, classifying them into 11 groups based on epitope specificity. MAbs from six groups (subgroups I-1, I-2, and II-1, as well as groups III, IV, and V, collectively comprising 15 MAbs) recognized six distinct epitopes within the C-terminus of p30 (137–194 aa), while MAb 16-5E7E8 (subgroup II-2) recognized a distinct epitope within residues 154–190. The p30 C-terminus (137–194 aa) demonstrated structural stability under denaturing conditions (SDS–PAGE and western blot [WB]) and was identified as an immunodominant region through its reactivity with ASFV-positive sera. Notably, MAbs targeting this immunodominant region exhibited modest neutralizing activity, whereas those binding to other regions showed no neutralizing activity. Furthermore, MAb Q4-1F10B7 (group VI) recognized a linear epitope “116TSSFETLFEQ125,” MAbs Q1-1G4B2 (group VIII) and Q8-4F9F3 (group IX) recognized conformation-dependent epitopes, and MAb 5-1C8B6 (group VII) likely recognized an aberrant or non-native form of the p30 protein. These results expand the epitope landscape of p30 protein and lay a foundation for ASF diagnosis and vaccine research.

分类号：

  • 相关文献

[1]Preparation and epitope mapping of monoclonal antibodies against African swine fever virus P30 protein. Zhou, Gaijing,Shi, Zhengwang,Luo, Juncong,Cao, Liyan,Yang, Bo,Wan, Ying,Wang, Lijuan,Song, Rui,Ma, Yuan,Tian, Hong,Zheng, Haixue. 2022

[2]Epitope mapping and establishment of a blocking ELISA for mAb targeting the p72 protein of African swine fever virus. Huan Cheng Liao,Zheng Wang Shi,Gai Jing Zhou,Jun Cong Luo,Wan Ying Wang,Lu Feng,Fan Zhang,Xin Tai Shi,Hong Tian,Hai Xue Zheng. 2024

[3]Identification of immunodominant epitope on African swine fever virus K145R protein using monoclonal antibodies. Weldu Tesfagaber,Lezi Hou,Wan Wang,Yuanmao Zhu,Encheng Sun,Jingli Liu,Taijie Guo,Renqiang Liu,Zhigao Bu,Fang Li,Dongming Zhao. 2025

[4]Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus. Jingjing Ding,Jifei Yang,Daoyuan Jiang,Yanyang Zhou,Chenxi Li,Yanhua Li. 2022

[5]Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus. Gao Y.,Xia T.,Bai J.,Zhang L.,Zheng H.,Jiang P.. 2022

[6]Identification of p72 epitopes of African swine fever virus and preliminary application. Chun Miao,Sicheng Yang,Junjun Shao,Guangqing Zhou,Yunyun Ma,Shenghui Wen,Zhuo Hou,Decai Peng,Hui Chen Guo,Wei Liu,Huiyun Chang. 2023

[7]Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies. Dan Yin,Renhao Geng,Hongxia Shao,Jianqiang Ye,Kun Qian,Hongjun Chen,Aijian Qin. 2022

[8]A highly efficient blocking ELISA based on p72 monoclonal antibody for the detection of African swine fever virus antibodies and identification of its linear B cell epitope. Weldu Tesfagaber,Wan Wang,Lulu Wang,Rui Zhao,Yuanmao Zhu,Fang Li,Encheng Sun,Renqiang Liu,Zhigao Bu,Geng Meng,Dongming Zhao. 2024

[9]禽网状内皮组织增生症病毒p30蛋白的表达及检测. 张石磊,于圣青,陈鸿军,丁铲. 2011

[10]表达非洲猪瘟病毒p30蛋白的重组新城疫病毒在小鼠中的免疫原性. 陈新新,吉艳红,刘彬,李小阳,殷宏,朱启运. 2015

[11]禽网状内皮组织增生症病毒p30-gp90串联表达蛋白及其免疫原性. 陈瑞爱,李延鹏,郭凯,刘正伟. 2014

[12]非洲猪瘟病毒p30蛋白与CRM197的融合表达及对小鼠的免疫原性评价. 张琦,董宁宁,谈晓梅,石正旺,李娜,朱雯琪,汤傲星,李传锋,朱杰,刘光清,苏艳,孟春春. 2024

[13]BRD4和ZNF76协同调控ASFV p30表达促进病毒复制的机制研究. 陈迎恩,赵亚茹,陈奕康,孙华林,张忠辉,杨吉飞,关贵全,殷宏,牛庆丽. 2025

[14]Identification of a New Antigen Epitope in the Nuclear Localization Signal Region of Porcine Circovirus Type 2 Capsid Protein. Guo, Longjun,Lu, Yuehua,Huang, Liping,Wei, Yanwu,Liu, Changming. 2011

[15]Identification of a novel B cell epitope on the nucleocapsid protein of porcine reproductive and respiratory syndrome virus by phage display. An, TQ,Zhou, YJ,Qiu, HJ,Tong, GZ,Wang, YF,Liu, JX,Yang, JY. 2005

[16]Mapping of epitopes of VP2 protein of chicken anemia virus using monoclonal antibodies. Wang, Xiaoyan,Gao, Honglei,Gao, Yulong,Fu, Chaoyang,Wang, Zhi,Lu, Guili,Cheng, Yu,Wang, Xiaomei. 2007

[17]Identification of a conserved linear B-cell epitope at the N-terminus of the E2 glycoprotein of Classical swine fever virus by phage-displayed random peptide library. Peng, Wu-Ping,Hou, Qiang,Xia, Zhao-He,Chen, Dan,Li, Na,Sun, Yuan,Qiu, Hua-Ji. 2008

[18]Identification and characterization of a common B-cell epitope on EIAV capsid proteins. Hu, Zhe,Chang, Hao,Chu, Xiaoyu,Li, Shuang,Wang, Meiyue,Wang, Xiaojun.

[19]Identification of a Novel Linear B-Cell Epitope in the M Protein of Avian Infectious Bronchitis Coronaviruses. Xing, Junji,Liu, Shengwang,Han, Zongxi,Shao, Yuhao,Li, Huixin,Kong, Xiangang.

[20]Fine Mapping of a Foot-and-mouth Disease Virus Epitope Recognized by Serotype-Independent Monoclonal Antibody 4B2. Yu, Yongzhong,Wang, Haiwei,Zhao, Lei,Zhang, Chunyuan,Jiang, Zhigang,Yu, Li.

作者其他论文 更多>>