数字农科院2.0

African swine fever virus pS273R antagonizes stress granule formation by cleaving the nucleating protein G3BP1 to facilitate viral replication

文献类型： 外文期刊

作者： Tingting Li;Xuewen Li;Xiao Wang;Xin Chen;Gaihong Zhao;Chuanxia Liu;Miaofei Bao;Jie Song;Jiangnan Li;Li Huang;Jun Rong;Kegong Tian;Junhua Deng;Jianzhong Zhu;Xuehui Cai;Zhigao Bu;Jun Zheng;Changjiang Weng

作者机构：

关键词： African swine fever virus;G3BP1;IFN production;pS273R;stress granules;viral replication

期刊名称： Journal of Biological Chemistry

ISSN： 0021-9258

年卷期： 2023 年 299 卷 7 期

页码：

收录情况： SCIE（2023版） ; ; EI（2023版）

摘要： Cytoplasmic stress granules (SGs) are generally triggered by stress-induced translation arrest for storing mRNAs. Recently, it has been shown that SGs are regulated by different stimulators including viral infection, which is involved in the antiviral activity of host cells to limit viral propagation. To survive, several viruses have been reported to execute various strategies, such as modulating SG formation, to create optimal surroundings for viral replication. African swine fever virus (ASFV) is one of the most notorious pathogens in the global pig industry. However, the interplay between ASFV infection and SG formation remains largely unknown. In this study, we found that ASFV infection inhibited SG formation. Through SG inhibitory screening, we found that several ASFV-encoded proteins are involved in inhibition of SG formation. Among them, an ASFV S273R protein (pS273R), the only cysteine protease encoded by the ASFV genome, significantly affected SG formation. ASFV pS273R interacted with G3BP1 (Ras-GTPase-activating protein [SH3 domain] binding protein 1), a vital nucleating protein of SG formation. Furthermore, we found that ASFV pS273R cleaved G3BP1 at the G140–F141 to produce two fragments (G3BP1-N1–140 and G3BP1-C141–456). Interestingly, both the pS273R-cleaved fragments of G3BP1 lost the ability to induce SG formation and antiviral activity. Taken together, our finding reveals that the proteolytic cleavage of G3BP1 by ASFV pS273R is a novel mechanism by which ASFV counteracts host stress and innate antiviral responses.

分类号：

  • 相关文献

[1]TGEV NSP1 enhances viral replication through antagonizing stress granule formation. Yutong Tang,Yue Zhang,Ning Yang,Han Shi,Yuguang Fu,Bingrong Bai,Baoyu Li,Bin Yang,Guangliang Liu. 2025

[2]African Swine Fever Virus Cysteine Protease pS273R Inhibits Type I Interferon Signaling by Mediating STAT2 Degradation. Li, Yu-Hui,Peng, Jiang-Ling,Xu, Zhi-Sheng,Xiong, Mei-Guang,Wu, Huang-Ning,Wang, Su-Yun,Li, Dan,Zhu, Guo-Qiang,Ran, Yong,Wang, Yan-Yi. 2023

[3]OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein. Hualin Sun,Mengli Wu,Zhonghui Zhang,Yiwang Wang,Jifei Yang,Zhijie Liu,Guiquan Guan,Jianxun Luo,Hong Yin,Qingli Niu. 2023

[4]The antibodies against the A137R protein drive antibody-dependent enhancement of African swine fever virus infection in porcine alveolar macrophages. Xiaoke Yang,Encheng Sun,Huanjie Zhai,Tao Wang,Shida Wang,Yuxuan Gao,Qinghe Hou,Xiangyu Guan,Shuwen Li,Lian Feng Li,Hongxia Wu,Yuzi Luo,Su Li,Yuan Sun,Dongming Zhao,Yongfeng Li,Hua Ji Qiu. 2024

[5]非洲猪瘟病毒pS273R蛋白酶功能初探. 吴海燕,王子涵,刘英楠,陈宗艳,刘静宜,陈鸿军. 2023

[6]鸡G3BP1在新城疫病毒感染诱导应激颗粒形成过程中的作用. 张频,孙英杰,郑航,毛旭明,仇旭升,谭磊,孟春春,宋翠萍,廖瑛,丁铲,马卫明. 2017

[7]G3BP1基因在鸡肌内前脂肪细胞增殖与分化中的作用及其分子标记鉴定. 李远方,吴冉,李帅浩,魏千然,王亚东,王丹丹,李智,李国喜,刘翘铭. 2025

[8]Antiviral activity and underlying mechanisms of baicalin against avian infectious bronchitis virus in vitro. Feng, Haipeng,Zhang, Kai,Zhang, Kang,Guo, Zhiting,Liu, Qin,Wang, Lei,Wang, Xuezhi,Qiu, Zhengying,Wang, Guibo,Zhang, Jingyan,Li, Jianxi. 2022

[9]The African swine fever virus protease pS273R inhibits DNA sensing cGAS-STING pathway by targeting IKKε. Jia Luo,Jiajia Zhang,Jinghua Ni,Sen Jiang,Nengwen Xia,Yiwen Guo,Qi Shao,Qi Cao,Wanglong Zheng,Nanhua Chen,Quan Zhang,Hongjun Chen,Qing Chen,Hongfei Zhu,François Meurens,Jianzhong Zhu. 2022

[10]Newcastle disease virus induces stable formation of bona fide stress granules to facilitate viral replication through manipulating host protein translation. Sun, Yingjie,Dong, Luna,Yu, Shengqing,Wang, Xiaoxu,Zheng, Hang,Zhang, Pin,Meng, Chunchun,Zhan, Yuan,Tan, Lei,Song, Cuiping,Qiu, Xusheng,Liao, Ying,Ding, Chan,Dong, Luna,Zheng, Hang,Wang, Xiaoxu,Wang, Guijun,Ding, Chan.

[11]Newcastle Disease Virus Induces Stable F.ormation Of Bona Fide S tress Granules To Facilitate Viral Replication Through Manipulating Host Protein Translation. Sun, YJ, Dong, LN, Yu, SQ, Wang, XX, Zheng, H, Zhang, P, Meng, CC, Zhan, Y, Tan, L, Song, CP, Qiu, XS, Wang, GJ, Liao, Y, Ding, C. 2017

[12]Research Progress on the Structure and Function of G3BP. Weifang Kang,Yue Wang,Wenping Yang,Jing Zhang,Haixue Zheng,Dan Li. 2021

[13]Influenza a virus NS1 protein induced A20 contributes to viral replication by suppressing interferon-induced antiviral response. Feng, Wenjing,Sun, Xiaoning,Zhang, Maolin,Guan, Zhenhong,Duan, Ming,Shi, Ning.

[14]Integrin v1 Modulation Affects Subtype B Avian Metapneumovirus Fusion Protein-mediated Cell-Cell Fusion and Virus Infection. Yun, Bing-Ling,Guan, Xiao-Lu,Liu, Yong-Zhen,Zhang, Yao,Wang, Yong-Qiang,Qi, Xiao-Le,Cui, Hong-Yu,Liu, Chang-Jun,Zhang, Yan-Ping,Gao, Hong-Lei,Gao, Li,Li, Kai,Gao, Yu-Long,Wang, Xiao-Mei,Wang, Xiao-Mei.

[15]Effect of Nonstructural Protein 2 Hypervariable Regions in the Replication of Porcine Reproductive and Respiratory Syndrome Virus in Marc-145 Cells. Liu, Ying,Wang, Feng-Xue,Wen, Yang -Jun,Li, Zhen-Guang,Liu, Xing,Sun, Na,Yang, Yong,Zhang, Shu-Qin,Zhu, Hong-Wei,Cheng, Shi-Peng,Wu, Hua.

[16]Atypical Classical Swine Fever Infection Changes Interleukin Gene Expression in Pigs. Sun, Y. K.,Sun, Y. K.,Zhang, X. M.,Du, M.,Li, Y. X.,Pan, H. B.,Yan, Y. L.,Yang, Y. A..

[17]A single amino acid in the C-terminus of VP3 protein influences the replication of attenuated infectious bursal disease virus in vitro and in vivo. Wang, Yongqiang,Qi, Xiaole,Kang, Zhonghui,Yu, Fei,Qin, Liting,Gao, Honglei,Gao, Yulong,Wang, Xiaomei. 2010

[18]The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection. Zhang, Lizhou,Li, Hui,Chen, Yuming,Gao, Xiang,Lu, Zhen,Gao, Li,Wang, Yongqiang,Gao, Yulong,Gao, Honglei,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Pan, Qing,Qi, Xiaole,Wang, Xiaomei,Wang, Xiaomei.

[19]Eukaryotic translational initiation factor 4AII reduces the replication of infectious bursal disease virus by inhibiting VP1 polymerase activity. Gao, Li,Li, Kai,Zhong, Li,Zhang, Lizhou,Qi, Xiaole,Wang, Yongqiang,Gao, Yulong,Wang, Xiaomei.

[20]Early Growth Response Gene-1 Suppresses F.oot-And-Mouth Disease Virus Replication B y Enhancing Type I Interferon Pathway Signal Transduction. Zhu, ZX,Du, XL,Li, PF,Zhang, XL,Yang, F,Cao, WJ,Tian, H,Zhang, KS,Liu, XT,Zheng, HX. 2018

作者其他论文 更多>>