数字农科院2.0

BmTrpA1-Mediated ILP/AKT/FOXO signaling Underlies Temperature-Dependent diapause in Silkworms, Bombyx mori

文献类型： 外文期刊

作者： Yizhong Zhang;Haoping She;Shanshan Wang;Qianyi Cui;Wenjie Zhang;Shunming Tang;Xingjia Shen;Juan Zhu

作者机构：

关键词： AKT/FOXO signaling axis;BmTrpA1;Bombyx mori;Diapause hormone;ILP;RNAi

期刊名称： Journal of Insect Physiology

ISSN： 0022-1910

年卷期： 2026 年 169 卷

页码：

收录情况： SCIE（2025版）

摘要： Diapause is a developmentally arrested state in insects induced by seasonal cues such as photoperiod and temperature. The scientific literature agrees on that the temperature-sensitive transient receptor potential ankyrin 1 (TRPA1) channel and insulin signaling pathway (ILP/AKT/FOXO) are both essential for diapause regulation, but their interactions remain unclear. This study investigates whether the ILP/AKT/FOXO pathway can mediate the temperature-dependent maternal embryonic diapause induced by the TRPA1 channel in Bombyx mori . We found that the maternal pupae primed for diapause-egg-production (25DD) and those primed for non-diapause-egg-production (15DD) significantly differ in temporal patterns of BmTrpA1 gene expression. Next, we used the BmTrpA1 -/- mutant, and showed that the mutation results in abolishing the transient peak of diapause hormone (DH) concentration in hemolymph, affects the protein profile of ILP, mRNA profiles of AKT and FOXO , and phosphorylation of FOXO protein. Generally, the profiles in BmTrpA1 -/- mutants were similar to those of 15DD pupae. In addition, we found that RNAi-mediated silencing of FOXO in the BmTrpA1 -/- mutant, or AKT in 15DD pupae, reduces or increases the diapause incidence, respectively. Collectively, our results indicate that the temperature-dependent diapause regulation by the TRPA1 channel is mediated by the ILP/AKT/FOXO pathway in the silkworm.

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