数字农科院2.0

Multiplex CRISPR/Cas9 editing of the long‐chain acyl‐CoA synthetase family in the diatom Phaeodactylum tricornutum reveals that mitochondrial ptACSL3 is involved in the synthesis of storage lipids

文献类型： 外文期刊

作者： Xiahui Hao; Wenchao Chen; Alberto Amato; Juliette Jouhet; Eric Maréchal; Daniel Moog; Hanhua Hu; Hu Jin; Lingjie You; Fenghong Huang; Moosburner Mark; Andrew E. Allen; Yangmin Gong

关键词： Fatty acid; Long-chain acyl-CoA synthetase; Multiplexed CRISPR/Cas9; Phaeodactylum tricornutum; Triacylglycerol

期刊名称： NEW PHYTOLOGIST

ISSN： 0028-646X

年卷期： 2021 年

页码：

收录情况： JCR（2021版）

摘要：  Long-chain acyl-CoA synthetases (LACSs) play diverse and fundamentally important roles in lipid metabolism. While their functions have been well-established in bacteria, yeast and plants, the mechanisms by which LACS isozymes regulate lipid metabolism in unicellular oil-producing microalgae, including the diatom Phaeodactylum tricornutum, remain largely unknown.  In P. tricornutum, a family of five genes (ptACSL1~5) encodes LACS activities. We generated single lacs knockout/knockdown mutants using multiplexed CRISPR/Cas9 method, and determined their substrate specificities towards different fatty acids (FAs) and subcellular localizations.  ptACSL3 is localized in the mitochondria and its disruption led to compromised growth and reduced triacylglycerol (TAG) content when cells were bubbled with air. The ptACSL3 mutants showed altered FA profiles in two galactoglycerolipids and phosphatidylcholine (PC) with significantly reduced distribution of 16:0 and 16:1. ptACSL5 is localized in the peroxisome and its knockdown resulted in reduced growth rate and altered molecular species of PC and TAG, indicating a role in controlling the composition of acyl-CoAs for lipid synthesis.  Our work demonstrates the potential of generating gene knockout mutants with the mutation of large fragment deletion using multiplexed CRISPR/Cas9 and provides insight into the functions of LACS isozymes in lipid metabolism in the oleaginous microalgae.

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