数字农科院2.0

On-site screening method for bioavailability assessment of the organophosphorus pesticide, methyl parathion, and its primary metabolite in soils by paper strip biosensor

文献类型： 外文期刊

作者： Zhao Ma;Yuanbo Li;Chao Lu;Meng Li

作者机构：

关键词： Organophosphorus pesticide;p-nitrophenol;Paper strip biosensor;Screening method;Soil

期刊名称： Journal of Hazardous Materials

ISSN： 0304-3894

年卷期： 2023 年 457 卷

页码：

收录情况： SCIE（2023版） ; ; EI（2023版）

摘要： An important public concern worldwide is soil pollution caused by organophosphorus pesticides and their primary metabolites. To protect the public's health, screening these pollutants on-site and determining their soil bioavailability is important, but doing so is still challenging. This work improved the already-existing organophosphorus pesticide hydrolase (mpd) and transcriptional activator (pobR), and it first designed and constructed a novel biosensor (Escherichia coli BL21/pNP-LacZ) that can precisely detect methyl parathion (MP) and its primary metabolite p-nitrophenol with low background value. To create a paper strip biosensor, E. coli BL21/pNP-LacZ was fixed to filter paper using bio-gel alginate and sensitizer polymyxin B. According to the calibrations of the paper strip biosensor for soil extracts and standard curve, the color intensity of the paper strip biosensor collected by the mobile app may be used to compute the concentration of MP and p-nitrophenol. This method's detection limits were 5.41 µg/kg for p-nitrophenol and 9.57 µg/kg for MP. The detection of p-nitrophenol and MP in laboratory and field soil samples confirmed this procedure. Paper strip biosensor on-site allows for the semi-quantitative measurement of p-nitrophenol and MP levels in soils in a simple, inexpensive, and portable method.

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