数字农科院2.0

Novel Temporal Expression Patterns of EBF-Binding Proteins in Wing Morphs of The Grain Aphid Sitobion miscanthi

文献类型： 外文期刊

作者： Siyu Zhang;Qian Zhang;Xin Jiang;Qian Li;Yaoguo Qin;Wenkai Wang;Jia Fan;Julian Chen

作者机构：

关键词： antenna;E-β-farnesene (EBF);expression pattern;odorant binding protein;Sitobion miscanthi;temporal expression

期刊名称： Frontiers in Physiology

ISSN： 1664-042X

年卷期： 2021 年 12.0 卷

页码：

收录情况： JCR（2021版）

摘要： High chemosensitivity of insects to volatile organic compounds (VOC) stimuli is mediated by odorant binding proteins (OBPs). In aphids, three OBPs (OBP3, OBP7 and OBP9) are E-β-farnesene (EBF)-binding proteins. Winged aphids are generally more sensitive than wingless aphids to VOCs, thus, wing presence is a phenotypic correlate of olfaction sensitivity. Here, we investigate the detailed temporal expression of these EBF-binding proteins and two other OBPs (OBP6 and OBP10), in the grain aphid Sitobion miscanthi 0 h, 2 h, 1 day, 3 days, 10 days, and 20 days after adult emergence. Both winged and wingless aphids were examined to further uncover phenotypic specification. Then, the expression patterns before and after EBF induction were analyzed. Throughout adulthood, only OBP7 had significantly higher antennal expression in winged aphids; however, there was no significant difference in the antennal expression of OBP3 between wing morphs at most time points. Except it was lower in newly emerged winged aphids but increased rapidly to the same level in wingless aphids at 1 day. OBP9 did not differ in expression between the morphs and was the only OBP that did not exhibit an expression trough at the beginning of the adult stage (0 h). The expression of OBP9 remained relatively stable and high throughout the adult stage in both phenotypes, showing the highest level among the three EBF-binding proteins. After EBF induction, its expression was further up-regulated in both morphs. Therefore, this protein may be an important molecule for EBF recognition in aphids. OBP7 strongly responded to EBF but only in winged aphids, suggesting that this protein is important in the more sensitive EBF recognition process of winged aphids. In addition, the antennal expression level of OBP3 did not respond to EBF induction. These findings revealed a temporal expression pattern of OBPs in aphids and showed that figuring out the pattern is critical for correctly selecting morphs and sampling times, which will support the discovery of reliable findings and allow solid conclusions to be drawn. Our findings also inspire on the interaction mode of the three EBF-binding proteins in relation to EBF perception in aphids.

分类号：

  • 相关文献

[1]A Detailed Spatial Expression Analysis of Wing Phenotypes Reveals Novel Patterns of Odorant Binding Proteins in the Soybean Aphid, Aphis glycines. Ling Wang;Hang Yin,Zhiguo Zhu,Shuai Yang,Jia Fan. 2021

[2]Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze). Gu, Shao-Hua,Zhang, Xue-Ying,Wu, Kong-Ming,Guo, Yu-Yuan,Zhang, Yong-Jun,Wang, Shu-Ping,Zhou, Jing-Jiang.

[3]FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE). Wang, Gui-Rong,Zhang, Xue-Ying,Guo, Yu-Yuan,Zhang, Yong-Jun,Wang, Wei-Xuan,Zhang, Ziding,Zhou, Jing-Jiang. 2011

[4]Cloning, expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug, Adelphocoris lineolatus (Goeze). Gu ShaoHua,Sun Yang,Ren LiYan,Zhang XueYing,Zhang YongJun,Wu KongMing,Guo YuYuan,Sun Yang,Ren LiYan. 2010

[5]Cetyltriethylammonium bromide stimulating transcription of Bombyx mori nucleopolyhedrovirus gp64 gene promoter mediated by viral factors. Zhou, YJ,Yi, YZ,Zhang, ZF,He, JL,Zhang, YX.

[6]Antennal morphology, structure and sensilla distribution in Microplitis pallidipes (Hymenoptera : Braconidae). Luo, Li-Zhi,Hammond, Abner. 2007

[7]Candidate chemosensory genes identified in Colaphellus bowringi by antennal transcriptome analysis. Li, Xiao-Ming,Zhu, Xiu-Yun,Wang, Zhi-Qiang,Wang, Yi,Chen, Geng,Deng, Dao-Gui,Zhang, Ya-Nan,He, Peng,Sun, Liang. 2015

[8]De novo assembly and characterization of antennal transcriptome reveal chemosensory system in Nysius Mate. Zhang, Ya-Nan,Zhu, Xiu-Yun,Zhang, Qian,Yin, Cai-Yun,Deng, Dao-Gui,Li, Xiao-Ming,Sun, Liang,Dong, Zhi-Ping,Zuo, Ling-Hua. 2016

[9]Ultrastructural and Descriptive Study on the Adult Body Surface of Heortia vitessoides (Lepidoptera: Crambidae). Lei Liu,Yan Zhang,Shan Chun Yan,Bin Yang,Gui Rong Wang. 2023

[10]Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances. Ke L.,Chen X.,Dai P.,Liu Y.-J.. 2023

[11]SNMP1 is critical for sensitive detection of the desert locust aromatic courtship inhibition pheromone phenylacetonitrile. Joris Lehmann,Yannick Günzel,Maryam Khosravian,Sina Cassau,Susanne Kraus,Johanna S. Libnow,Hetan Chang,Bill S. Hansson,Heinz Breer,Einat Couzin-Fuchs,Joerg Fleischer,Jürgen Krieger. 2024

[12]Fitness Cost of the Field-Evolved Resistance to Sulfoxaflor and Multi-Insecticide Resistance of the Wheat Aphid Sitobion miscanthi (Takahashi). Li X.,Zhu S.,Li Q.,Sun Y.,Wang Y.,Tian X.,Ran X.,Li X.,Zhang Y.,Gao H.,Zhu X.. 2023

[13]Effect Of The Secondary Symbiont H.amiltonella Defensa On Fitness A nd Relative Abundance Of Buchnera Aphidicola Of Wheat Aphid, Sitobion Miscanthi. Li Qian,Fan Jia,Li Qian,Sun Jingxuan,Wang Manqun,Chen Julian. 2018

[14]Proteomic and Transcriptomic Analysis for Identification of Endosymbiotic Bacteria Associated with BYDV Transmission Efficiency by Sitobion miscanthi. Wenjuan Yu,Emilie Bosquée,Jia Fan,Yong Liu,Claude Bragard,Frédéric Francis,Julian Chen. 2023

[15]The Reciprocal Effect of Elevated CO2 and Drought on Wheat-Aphid Interaction System. Haicui Xie,Fengyu Shi,Jingshi Li,Miaomiao Yu,Xuetao Yang,Yun Li,Jia Fan. 2022

[16]Screening and Evaluation for Antixenosis Resistance in Wheat Accessions and Varieties to Grain Aphid, Sitobion miscanthi (Takahashi) (Hemiptera: Aphididae). Gebretsadik K.G.,Zhang Y.,Chen J.. 2022

[17]Can laboratory-reared aphid populations reflect the thermal performance of field populations in studies on pest science and climate change biology?. Wang, Xue-Jing,Ma, Chun-Sen. 2022

[18]Challenge generality of prediction based on Jensen's inequality: Moderate and large temperature fluctuations can lead to opposite performance deviation at high mean temperature. Wang, Xue-Jing,Ma, Chun-Sen. 2022

[19]Activation of Wheat Defense Response by Buchnera aphidicola-Derived Small Chaperone Protein GroES in Wheat Aphid Saliva. Qian Li,Yu Fu,Xiaobei Liu,Jingxuan Sun,Maolin Hou,Yong Zhang,Julian Chen. 2022

[20]Effects of Different Temperatures on the Development and Reproduction of Sitobion miscanthi From Six Different Regions in China. Jingxuan Sun,Xiaoling Tan,Qian Li,Frédéric Francis,Julian Chen. 2022

作者其他论文 更多>>