数字农科院2.0

Identification of the NPF gene family in melon and functional analysis of CmNPF7.3 in conferring salt tolerance

文献类型： 外文期刊

作者： Bing Xue;Ming Li;Yang Xue;Linlong Zhang;Fangfang Wang;Jie Li;Shengjie Zhao;Zhenyu Huang;Zhihong Xu;Pingyong Wang

作者机构：

关键词： CmNPF7.3;Gene family;Melon;Nitrate;NPF;Salt stress

期刊名称： Scientia Horticulturae

ISSN： 0304-4238

年卷期： 2025 年 353 卷

页码：

收录情况： SCIE（2025版）

摘要： Salt stress is one of the major abiotic stresses that affect the yield and quality of melon (Cucumis melo L.), posing a serious threat to the healthy and sustainable development of the melon industry. In plants, the nitrate transporter 1/peptide transporter family (NPF) proteins are important nitrate transporters that play a significant role in regulating nitrate absorption and distribution, thereby improving nitrogen use efficiency. In addition, some NPF genes can mediate the transmembrane transport of peptides and hormones, thereby regulating plant growth and development, and enhancing resistance to external stress, particularly salt stress. In this study, 56 NPF family members were identified in melon. CmNPFs were classified into eight subfamilies, and numerous cis-acting elements in their promoter regions were associated with hormone signaling and external stress responses. Among them, CmNPF7.3 was found to be significantly upregulated in the tolerant accession under salt stress but downregulated in the sensitive accession. Subcellular localization analysis showed that CmNPF7.3 was located to the plasma membrane. Overexpression of CmNPF7.3 significantly increased NO3− concentration in both roots and shoots, enhanced antioxidant capacity and photosynthetic efficiency under salt stress, and improved plant salt tolerance. This study provided valuable insights into the melon NPF gene family and established a foundation for future validation of molecular mechanisms and genetic applications.

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