数字农科院2.0

Characterization and Vaccine Potential of EtRON2L1-1, a Novel RON2 Paralog in Eimeria tenella

文献类型： 外文期刊

作者： Wenqiang Tang;Manyu Liu;Xialing Zhao;Bin Shi;Wanxiang Qi;Hui Dong;Kun Li

作者机构：

关键词： Eimeria tenella;Host cell invasion;Rhoptry neck protein;Vaccine candidate

期刊名称： Pakistan Veterinary Journal

ISSN： 0253-8318

年卷期： 2025 年 45 卷 4 期

页码：

收录情况： SCIE（2025版）

摘要： Eimeria (E.) tenella, the causative agent of avian coccidiosis, employs apical complex proteins like RON2 for host cell invasion. While EtRON2 has been well-studied, its paralogs remain poorly characterized. This study investigated EtRON2L1-1, a RON2 homolog, for its structural features, stage-specific expression, and vaccine potential. Bioinformatic analysis revealed EtRON2L1-1 shares only 23.46% amino acid identity with EtRON2, lacks a signal peptide, and contains three transmembrane domains. Expression profiling revealed distinct transcriptional and translational regulations, with peak mRNA levels in sporulated oocysts and highest protein expression in sporozoites, indicative of post-transcriptional control. Immunofluorescence studies showed stage-dependent localization patterns: cytoplasmic distribution in free sporozoites transitioning to apical end during host cell invasion. In vitro neutralization assays established EtRON2L1-1 's involvement in invasion, with specific antibodies exhibiting dose-dependent inhibition (13% at 50μg/mL to 42% at 400μg/mL). Vaccination trials demonstrated that while 100μg pCAGGS-EtRON2L1-1 significantly improved weight gain and reduced oocyst output compared to controls, its protective efficacy was inferior to pCAGGS-EtRON2L2, particularly in mitigating intestinal lesions. These findings characterize EtRON2L1-1 as a functionally distinct RON2 paralog involved in host cell invasion, with partial but promising vaccine potential that requires further optimization for effective coccidiosis control.

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