数字农科院2.0

Identification and Predictions Regarding the Biosynthesis Pathway of Polyene Macrolides Produced by Streptomyces roseoflavus Men-myco-93-63

文献类型: 外文期刊

作者: Han Xing;Wang Jiao;Liu Lianna;Shen Fengying;Meng Qingfang;Li Xing;Li Yaning;Liua Daqun

作者机构:

关键词: antibiotic; antifungal activity; biosynthetic gene cluster; polyene macrolide; Streptomyces roseoflavus Men-myco-93-63

期刊名称: Applied and Environmental Microbiology

ISSN: 0099-2240

年卷期: 2021 年 87 卷 10 期

页码:

收录情况: JCR(2021版) ; EI(2021版)

摘要: A group of polyene macrolides mainly composed of two constituentswas isolated from the fermentation broth of Streptomyces roseoflavus Men-myco-93-63, which was isolated from soil where potato scabs were repressed naturally. Oneof these macrolides was roflamycoin, which was first reported in 1968, and the otherwas a novel compound named Men-myco-A, which had one methylene unit morethan roflamycoin. Together, they were designated RM. This group of antibiotics exhibitedbroad-spectrum antifungal activities in vitro against 17 plant-pathogenic fungi, with50% effective concentrations (EC50) of 2.05 to 7.09mg/ml and 90% effective concentrations(EC90) of 4.32 to 54.45mg/ml, which indicates their potential use in plant diseasecontrol. Furthermore, their biosynthetic gene cluster was identified, and the associatedbiosynthetic assembly line was proposed based on a module and domain analysis ofpolyketide synthases (PKSs), supported by findings from gene inactivation experiments. © 2021, American Society for Microbiology. All Rights Reserved.

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