数字农科院2.0

The nematode effector calreticulin competes with the high mobility group protein OsHMGB1 for binding to the rice calmodulin-like protein OsCML31 to enhance rice susceptibility to Meloidogyne graminicola

文献类型： 外文期刊

作者： Liu, Jing;Zhang, Jiaqian;Wei, Ying;Su, Wen;Li, Wei;Wang, Bing;Peng, Deliang;Gheysen, Godelieve;Peng, Huan;Dai, Liangying

作者机构：

关键词： calmodulin-like protein;calreticulin;effector;Meloidogyne graminicola;OsHMGB1;plant defense

期刊名称： PLANT CELL AND ENVIRONMENT

ISSN： 0140-7791

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： The root-knot nematode Meloidogyne graminicola secretes effectors into rice tissues to modulate host immunity. Here, we characterised MgCRT1, a calreticulin protein of M. graminicola, and identified its target in the plant. In situ hybridisation showed MgCRT1 mRNA accumulating in the subventral oesophageal gland in J2 nematodes. Immunolocalization indicated MgCRT1 localises in the giant cells during parasitism. Host-induced gene silencing of MgCRT1 reduced the infection ability of M. graminicola, while over-expressing MgCRT1 enhanced rice susceptibility to M. graminicola. A yeast two-hybrid approach identified the calmodulin-like protein OsCML31 as an interactor of MgCRT1. OsCML31 interacts with the high mobility group protein OsHMGB1 which is a conserved DNA binding protein. Knockout of OsCML31 or overexpression of OsHMGB1 in rice results in enhanced susceptibility to M. graminicola. In contrast, overexpression of OsCML31 or knockout of OsHMGB1 in rice decreases susceptibility to M. graminicola. The GST-pulldown and luciferase complementation imaging assay showed that MgCRT1 decreases the interaction of OsCML31 and OsHMGB1 in a competitive manner. In conclusion, when M. graminicola infects rice and secretes MgCRT1 into rice, MgCRT1 interacts with OsCML31 and decreases the association of OsCML31 with OsHMGB1, resulting in the release of OsHMGB1 to enhance rice susceptibility.

分类号：

  • 相关文献

[1]The Root Knot Nematode Meloidogyne graminicola Regulates Rice Potassium Uptake by Targeting OsHAK17 to Facilitate Parasitism in Rice. Hongxiang Shi,Jiaqian Zhang,Maoyan Liu,Wei Li,Wenkun Huang,Deliang Peng,Huan Peng,Jing Liu. 2025

[2]ModulationofmousemacrophageproteomeinducedbyToxoplasmagondiitachyzoitesinvivo. ZhouDonghui,YuanZiguo,ZhaoFurong,LiHailong,ZhouYang,LinRuiqing,ZouFengcai,SongHuiqun,XuMinjun,andZhuXingquan. 2011

[3]SequencevariationinROP13geneamongToxoplasmagondiiisolatesfromdifferentgeographicallocationsandhosts. WangPeiyuan,ZhouYang,LiJuan,SongHuiqun,ZhouDonghui,XuMinjun,ZhuXingquan. 2011

[4]Fibrillarin RNA methylase is an interacting protein of Cryptosporidium parvum calmodulin-like protein (CpCML). Tiancong Sun,Yu Chen,Rongsheng Mi,Haiyan Gong,Shasha Zhou,Xiangan Han,Yan Huang,Zhaoguo Chen. 2022

[5]Tacml36, A Wheat Calmodulin-Like Protein, P.ositively Participates In An I mmune Response To Rhizoctonia Cerealis. Lu, L, Rong, W, Zhou, RH, Huo, NX, Zhang, ZY. 2019

[6]Isolation and identification of sporozoite membrane protein of Cryptosporidium parvum and evaluation of calmodulin-like protein immune protection. Huang, Yan,Chen, Yu,Liu, Yuxuan,Mi, Rongsheng,Han, Xiangan,Gong, Haiyan,Cheng, Long,Chen, Zhaoguo. 2022

[7]Molecular cloning of a new wheat calreticulin gene TaCRT1 and expression analysis in plant defense responses and abiotic stress resistance. Lin, R. M.,Wang, F. T.,Feng, J.,Xu, S. C.,An, Y. Q.,Xu, Y. F.. 2011

[8]Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Ding, Zhiping,Liu, Zewen,Liu, Shuhua,Yang, Baojun,Zhang, Chengwei.

[9]Sjcrt, A Recombinant Schistosoma Japonicum C.alreticulin, Induces Maturation Of D endritic Cells And A Th1-Polarized Immune Response In Mice. Ma, LZ, Li, DD, Yuan, CX, Zhang, XQ, Ta, N, Zhao, XC, Li, YM, Feng, XG. 2017

[10]Calreticulin Identified as One of the Androgen Response Genes That Trigger Full Regeneration of the Only Capable Mammalian Organ, the Deer Antler. Guo, Qianqian,Zheng, Junjun,Ba, Hengxing,Sun, Hongmei,Zhai, Jingjie,Wang, Wenying,Li, Chunyi. 2022

[11]Efficient genome editing in dicot plants using calreticulin promoter-driven CRISPR/Cas system. Bingjie Li,Yun Shang,Lixianqiu Wang,Jing Lv,Qi Wu,Fengjiao Wang,Jiangtao Chao,Jingjing Mao,Anming Ding,Xinru Wu,Kaili Xue,Chen Chen,Mengmeng Cui,Yuhe Sun,Huawei Zhang,Changbo Dai. 2025

[12]Evaluation Of Chinese Rice Varieties R.esistant To The Root- K not Nematode Meloidogyne Graminicola. Peng Huan,Peng De-liang,Kong Ling-an,Liu Shi-ming,Li Zhong-cai,Huang Wen-kun,Zhan Li-ping,Ding Zhong,Liu Ying. 2018

[13]Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections. Ji, Hong-li,Gheysen, Godelieve,Kyndt, Tina,Huang, Wen-kun,Ji, Hong-li,Debode, Jane. 2015

[14]Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation. Ji, Hong-Li,Gheysen, Godelieve,Kyndt, Tina,Huang, Wen-Kun,Ji, Hong-Li.

[15]Evaluation Of The Biocontrol Potential O.f Aspergillus Welwitschiae Against T he Root-Knot Nematode Meloidogyne Graminicola In Rice (Oryza Sativa L.). Huang Wen:kun,Xiang Chao,Peng Huan,Ding Zhong,Peng De:liang,Liu Shi:ming,Li Zhong:cai,Liu Ying,Kong Ling:an. 2019

[16]The development and molecular characterization of a rapid detection method for Rice root-knot nematode (Meloidogyne graminicola). Peng, Huan,Huang, Wenkun,Kong, Lingan,He, Wenting,Peng, Deliang,Holgado, Ricardo.

[17]Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice. Yongrui Niu,Liying Xiao,Janice de Almeida-Engler,Godelieve Gheysen,Deliang Peng,Xueqiong Xiao,Wenkun Huang,Gaofeng Wang,Yannong Xiao. 2022

[18]First Report of Meloidogyne graminicola on Rice in Anhui Province, China. Yuliang Ju,Xun Wu,Genjia Tan,Deliang Peng,Jinfeng Xu,Kun Qiu,Huiping Wu. 2021

[19]Alpha Beta-Dehydrocurvularin Isolated From The Fungus Aspergillus Welwitschiae Effectively Inhibited The Behaviour And Development Of The Root-Knot Nematode Meloidogyne Graminicola In Rice Roots. Xiang, C, Liu, Y, Liu, SM, Huang, YF, Kong, LA, Peng, H, Liu, MY, Liu, J, Peng, DL, Huang, WK. 2020

[20]Meloidogyne graminicola Population Structure in China Suggests a South-to-North Expansion. Liu, Mao-Yan,Shao, Hu-Die,Wu, Yang-Yan,Peng, De-Liang,Yu, Jing-Wen,Jia, Jian-Ping,Peng, Huan,Li, Chuan-Ren,Sulaiman, Abdulsalam,Yu, Xi-Yue,Li, Cai-Hong,Huang, Wen-Kun. 2023

作者其他论文 更多>>