Comparative analysis of the genetic diversity of the neutral microsatellite loci and second exon of the goat MHC-DQB1 gene
文献类型: 外文期刊
作者: Gong, Ying;Guo, Yi;He, Yong-Meng;Yuan, Ying;Yang, Bai-Gao;Duan, Xing-Hai;Liu, Cheng-Li;Zhang, Jia-Hua;Hong, Qiong-Hua;Ma, Yue-Hui;Na, Ri-Su;Han, Yan-Guo;Zeng, Yan;Huang, Yong-Fu;Zhao, Yong-Ju;Zhao, Zhong-Quan;Guangxin, E.
作者机构:
关键词: Major histocompatibility complex (MHC); microsatellite markers; goats; genetic diversity; selection
期刊名称: ANIMAL BIOTECHNOLOGY
ISSN: 1049-5398
年卷期: 2021 年
页码:
收录情况: JCR(2021版) ; EI(2021版)
摘要: This study compared and analyzed the genetic diversity and population structure of exon 2 of the DQB1 gene and 13 autosomal neutral microsatellite markers from 14 Chinese goat breeds to explore the potential evolutionary mechanism of the major histocompatibility complex (MHC). A total of 287 haplotypes were constructed from MHC-DQB1 exon 2 from 14 populations, and 82 nucleotide polymorphic sites (SNPs, 31.78%) and 172 heterozygous individuals (79.12%) were identified. The F-ST values of the microsatellites and MHC-DQB ranged between 0.01831-0.26907 and 0.00892-0.38871, respectively. Furthermore, 14 goat populations showed rich genetic diversity in the microsatellite loci and MHC-DQB1 exon 2. However, the population structure and phylogenetic relationship represented by the two markers were different. Positive selection and Tajima's D test results showed the occurrence of a diversified selection mechanism, which was primarily based on a positive and balancing selection in goat DQB. This study also found that the DQB sequences of bovines exhibited trans-species polymorphism (TSP) among species and families. In brief, this study indicated that positive and balancing selection played a major role in maintaining the genetic diversity of DQB, and TSP of MHC in bovines was common, which enhanced the understanding of the MHC evolution.
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