数字农科院2.0

Nuclear ribonucleoprotein RALY downregulates foot-and-mouth disease virus replication but antagonized by viral 3C protease

文献类型： 外文期刊

作者： Wu, Jin'en;Sun, Chao;Guan, Junyong;Abdullah, Sahibzada Waheed;Wang, Xuefei;Ren, Mei;Qiao, Lu;Sun, Shiqi;Guo, Huichen

作者机构：

关键词： foot-and-mouth disease virus (FMDV);RALY;internal ribosome entry site (IRES);virus-host interactions;3C(pro)

期刊名称： MICROBIOLOGY SPECTRUM

ISSN： 2165-0497

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： The internal ribosome entry site (IRES) element constitutes a cis-acting RNA regulatory sequence that recruits the ribosomal initiation complex in a cap-independent manner, assisted by various RNA-binding proteins and IRES trans-acting factors. Foot-and-mouth disease virus (FMDV) contains a functional IRES element and takes advantage of this element to subvert host translation machinery. Our study identified a novel mechanism wherein RALY, a member of the heterogeneous nuclear ribonucleoproteins (hnRNP) family belonging to RNA-binding proteins, binds to the domain 3 of FMDV IRES via its RNA recognition motif residue. This interaction results in the downregulation of FMDV replication by inhibiting IRES-driven translation. Furthermore, our findings reveal that the inhibitory effect exerted by RALY on FMDV replication is not attributed to the FMDV IRES-mediated assembly of translation initiation complexes but rather to the impediment of 80S ribosome complex formation after binding with 40S ribosomes. Conversely, 3C(pro) of FMDV counteracts RALY-mediated inhibition by the ubiquitin-proteasome pathway. Therefore, these results indicate that RALY, as a novel critical IRES-binding protein, inhibits FMDV replication by blocking the formation of 80S ribosome, providing a deeper understanding of how viruses recruit and manipulate host factors. IMPORTANCE The translation of FMDV genomic RNA driven by IRES element is a crucial step for virus infections. Many host proteins are hijacked to regulate FMDV IRES-dependent translation, but the regulatory mechanism remains unknown. Here, we report for the first time that cellular RALY specifically interacts with the IRES of FMDV and negatively regulates viral replication by blocking 80S ribosome assembly on FMDV IRES. Conversely, RALY-mediated inhibition is antagonized by the viral 3C protease by the ubiquitin-proteasome pathway. These results would facilitate further understanding of virus-host interactions and translational control during viral infection.

分类号：

  • 相关文献

[1]Nuclear ribonucleoprotein RALY targets virus nucleocapsid protein and induces autophagy to restrict porcine epidemic diarrhea virus replication. Wenzhen Qin,Ning Kong,Yu Zhang,Sujie Dong,Huanjie Zhai,Xueying Zhai,Xinyu Yang,Chenqian Ye,Manqing Ye,Changlong Liu,Lingxue Yu,Hao Zheng,Hai Yu,Wen Zhang,Guangzhi Tong,Daoliang Lan,Wu Tong,Tongling Shan. 2022

[2]Foot-and-mouth disease virus downregulates vacuolar protein sorting 28 to promote viral replication. Wang, Xuefei,Abdullah, Sahibzada Waheed,Wu, Jin'en,Tang, Jianli,Zhang, Yun,Dong, Hu,Bai, Manyuan,Wei, Sumin,Sun, Shiqi,Guo, Huichen. 2023

[3]Foot-and-Mouth Disease Virus Induces Porcine Gasdermin E-Mediated Pyroptosis through the Protease Activity of 3C(pro). Ren, Xujiao,Yin, Mengge,Zhao, Qiongqiong,Zheng, Zixuan,Wang, Haoyuan,Lu, Zengjun,Li, Xiangmin,Qian, Ping. 2023

[4]eEF1A Interacts with the NS5A Protein and Inhibits the Growth of Classical Swine Fever Virus. Li, Su,Feng, Shuo,Wang, Jing-Han,He, Wen-Rui,Qin, Hua-Yang,Dong, Hong,Li, Lian-Feng,Yu, Shao-Xiong,Li, Yongfeng,Qiu, Hua-Ji. 2015

[5]Eef1G Interaction With Foot-And-Mouth Disease V.irus Nonstructural Protein 2B: I dentification By Yeast Two-Hybrid System. Zhang, ZW,Pan, L,Ding, YZ,Lv, JL,Zhou, P,Fang, YZ,Liu, XS,Zhang, YG,Wang, YL. 2017

[6]Ribosomal Protein L13 Participates in Innate Immune Response Induced by Foot-and-Mouth Disease Virus. Junyong Guan,Shichong Han,Jin’en Wu,Yun Zhang,Manyuan Bai,Sahibzada Waheed Abdullah,Shiqi Sun,Huichen Guo. 2021

[7]PFT-α inhibits gallid alpha herpesvirus 1 replication by repressing host nucleotide metabolism and ATP synthesis. Li Xu,Zhitao Wang,Zhijie Chen,Lu Cui,Zheyi Liu,Yumeng Liang,Xuefeng Li,Yanhui Zhang,Shengwang Liu,Hai Li. 2022

[8]Viral proteins suppress rice defenses by boosting OsTSN1 RNA decay via phase separation and multimerization. Ming Zeng,Shuai Fu,Youping Xu,Liyan Li,Dan Wang,Shi Bo Gao,Lianshun Zheng,Yunge Zhang,Cui Zhang,Shifang Fei,Xuan Ye,Lele Chen,Yaqin Wang,Tong Zhang,Xueping Zhou,Jianxiang Wu. 2025

[9]Ecological drivers and functional roles of phage communities in the Yangtze River's freshwater ecosystems. Liang Peng,Fengxia Yang,Jing Zhang,Jiayu Shang,Wei Xu,Shoushan Sheng,Qinfen Li,Yukun Zou,Zhengfu Yue. 2025

[10]Quantitative proteomics by amino acid labeling in foot-and-mouth disease virus (FMDV)-infected cells. Ye, Yu,Xin, Chaoan,Liao, Ming,Fan, Huiying,Ye, Yu,Luo, Yongwen,Xin, Chaoan,Liao, Ming,Fan, Huiying,Ye, Yu,Xin, Chaoan,Liao, Ming,Fan, Huiying,Tong, Tiezhu,Yan, Guangrong,Yan, Guangrong,Liu, Xiangtao.

[11]Stable expression of foot-and-mouth disease virus protein VP1 fused with cholera toxin B subunit in the potato (Solanum tuberosum). He, Dong-Mei,Qian, Kai-Xian,Shen, Gui-Fang,Li, Yi-Na,Zhang, Zhi-Fang,Su, Zhong-Liang,Shao, Hong-Bo.

[12]Development of a serotype colloidal gold strip using monoclonal antibody for rapid detection type Asia1 foot-and-mouth disease. Lin, Tong,Shao, Jun-jun,Du, Jun-zheng,Cong, Guo-zheng,Gao, Shan-dian,Chang, Huiyun. 2011

[13]Expression and detection of the FMDV VP1 transgene and expressed structural protein in Arabidopsis thaliana. Pan, Li,Zhang, Yongguang,Wang, Yonglu,Lv, Jianliang,Zhou, Peng,Zhang, Zhongwang. 2011

[14]Molecular relationships between type Asia 1 new strain from China and type O Panasia strains of foot-and-mouth-disease virus. Li, Dong,Shang, You-Jun,Shang, Jun,Liu, Zai-Xin,Liu, Xiang-Tao,Cai, Xue-Peng. 2007

[15]Differential gene expression in porcine SK6 cells infected with wild-type and SAP domain-mutant foot-and-mouth disease virus. Ni, Zixin,Yang, Fan,Cao, Weijun,Zhang, Xiangle,Jin, Ye,Mao, Ruoqing,Du, Xiaoli,Li, Weiwei,Guo, Jianhong,Liu, Xiangtao,Zhu, Zixiang,Zheng, Haixue,Ni, Zixin. 2016

[16]The effect of bovine IFN-alpha on the immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus. Guo, HC,Liu, ZX,Sun, SQ,Leng, QW,Li, D,Liu, XT,Xie, QG. 2004

[17]The infectivity and pathogenicity of a foot-and-mouth disease virus persistent infection strain from oesophageal-pharyngeal fluid of a Chinese cattle in 2010. Bao, Hui-Fang,Li, Dong,Sun, Pu,Zhou, Qiang,Bai, Xing-Wen,Fu, Yuan-Fang,Lu, Zeng-Jun,Liu, Zai-Xin,Hu, Jun. 2011

[18]High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts. Li, Y,Sun, M,Liu, JX,Yang, ZQ,Zhang, ZF,Shen, GF. 2006

[19]The rescue and evaluation of FLAG and HIS epitope-tagged Asia 1 type foot-and-mouth disease viruses. Yang, Bo,Yang, Fan,Zhang, Yan,Liu, Huanan,Jin, Ye,Cao, Weijun,Zhu, Zixiang,Zheng, Haixue,Yin, Hong.

[20]3C(pro) of FMDV inhibits type II interferon-stimulated JAK-STAT signaling pathway by blocking STAT1 nuclear translocation. Wu, Xiangju,Chen, Lei,Sui, Chao,Hu, Yue,Jiang, Dandan,Yang, Fan,Miller, Laura C.,Li, Juntong,Cong, Xiaoyan,Hrabchenko, Nataliia,Lee, Changhee,Du, Yijun,Qi, Jing. 2023

作者其他论文 更多>>