数字农科院2.0

De novo biosynthesis of D-panthenol in engineered E. coli with rationally designed L-homoserine decarboxylase

文献类型： 外文期刊

作者： Peng Zheng;Jie Ren;Jie Zheng;Feixia Liu;Xinhao Han;Bo Yu

作者机构：

关键词： 3-Amino-1-propanol;D-panthenol;Enzyme rational design;L-tyrosine decarboxylase;Metabolic engineering

期刊名称： Metabolic Engineering

ISSN： 1096-7176

年卷期： 2025 年 92 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： D-panthenol is a compound of significant importance in the pharmaceutical, cosmetic, and nutraceutical sectors, attributed to its remarkable moisturizing, anti-inflammatory, and tissue repair properties. Traditional chemical synthesis encounters several challenges, including the generation of toxic by-products, low enantiomeric excess, and expensive purification processes. To date, complete biosynthesis of D-panthenol solely from glucose has seldom been documented. In this study, we have developed a new fermentative route to produce D-panthenol. The pathway incorporates previously unreported reaction of decarboxylating L-homoserine to 3-amino-1-propanol, which is achieved by rational design of a novel tyrosine decarboxylase mutant, informed by structural and mechanistic insights into enzymes acting on sterically similar substrates. The next enzyme facilitating the condensation of 3-amino-1-propanol with D-pantoate for D-panthenol formation was identified through a comprehensive screening of natural D-pantothenate synthetases. The artificial pathway was functionally expressed in a minimally engineered E. coli strain, resulting in the de novo production of D-panthenol from glucose. This research highlights a demonstration of an unnatural enzymatic synthesis process for D-panthenol. With further strain and process engineering, this new approach could be a promising way to produce D-panthenol biologically.

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