数字农科院2.0

Cross-regional dynamics of antibiotic resistance (AR): Genomic evidence of transmission for Escherichia coli carrying last-resort AR gene between food animals and humans in China

文献类型: 外文期刊

作者: Jie Hou;Ye Li;Qinglin Chen;Mengqi Liu;Yi An;Fengxia Yang;Yanpo Yao

作者机构:

关键词: Escherichia coli;Food animals;Genome sequences;Human beings;Last-resort antibiotic resistance genes;Transmission

期刊名称: Eco-Environment and Health

ISSN:

年卷期: 2025 年 5 卷 1 期

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收录情况: CSCD(2025-2026年度) ; ; ESCI(2025版)

摘要: The rapidly increasing dissemination of antibiotic-resistant bacteria (ARB) among food animals and humans is a burgeoning public health crisis. Yet, our understanding of ARB transmission is largely constrained to regional studies, with a dearth of insights into cross-regional dynamics. In this study, the whole-genome sequences of >800 E. coli strains harboring last-resort antibiotic resistance genes (LARGs) across 20 regions of China were retrieved from the NCBI RefSeq database. Our analysis uncovered that 1767 human–animal isolate pairs exhibited high homology, with the pairwise average nucleotide identity (ANI) values exceeding 99.5%. By employing phylogenetic analysis, transmission cluster identification, ANI, and single-nucleotide polymorphism (SNP) analysis, 42 human–animal transmission clusters and 306 recent transmission events were identified. Strikingly, more than 90% of these human–animal transmission events (284 out of 306) spanned different regions. Counterfactual analysis indicated that the cross-regional transmission significantly contributes to the prevalence of these ARBs in humans (21.62%), while its role in the food-animal epidemic is less pronounced (3.47%). Our findings underscore the urgency of a more integrated, nationwide approach to address the spread of LARG-harboring bacteria, which is essential to preserving the efficacy of our remaining antibiotic arsenal and safeguarding public health on a global scale.

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